Genetic determinants of bacterial fitness in polymicrobial wound infections

多种微生物伤口感染中细菌适应性的遗传决定因素

基本信息

批准号：
8806514
负责人：
Marvin Whiteley
金额：
$ 19.09万
依托单位：
UNIVERSITY OF TEXAS AT AUSTIN
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-02-15 至 2017-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8806514
关键词：
Acute Affect American Antibiotic Resistance Antibiotic Therapy Bacteria Bacterial Infections Burn injury Chronic Clinical Coculture Techniques Communities Decubitus ulcer Diabetic ulcer Exhibits Gene Expression Genes Genetic Genetic Determinism Genomics Goals Growth Healed Health Healthcare Systems High-Throughput Nucleotide Sequencing Hospitalization Human Infection Lead Metabolic Pathway Microbe Microbiology Modeling Molecular Mono-S Mus Organism Outcome Pathogenesis Pathway interactions Prevalence Process Protocols documentation Pseudomonas aeruginosa RNA Research Sampling Site Soft Tissue Infections Staphylococcus aureus Systemic infection Techniques Technology Therapeutic Virulence Wound Infection bacterial genetics base co-infection cost effective therapy fitness genome-wide healing in vivo insight knockout gene microbial mortality pathogen transcriptomics wound

项目摘要

DESCRIPTION (provided by applicant): Most bacterial pathogenesis studies have focused on mono-culture infections; however, it is clear that many bacterial infections are not simply the result of colonization with a single species, but rather ensue from the action of polymicrobial communities. Microbes within polymicrobial infections often display synergistic interactions that result in enhanced colonization, persistence and antibiotic resistance in the infection site. Such interactions have been particularly noted in polymicrobial soft tissue infections, such as those in burns and chronic wounds, although the molecular processes controlling these synergistic interactions are generally not known. Studies elucidating the genetic pathways involved in polymicrobial interactions that contribute to enhanced persistence in vivo is critical for a comprehensive understanding of synergy, and a necessary first step towards developing therapeutics to treat multi-species infections. The overall goal of this research plan is to examine, from a genomic standpoint, how interactions between opportunistic pathogens impact in vivo persistence in acute and chronic wound infections. To accomplish this goal, high-throughput genomics techniques will be employed to identify microbial genes that are required for and expressed during polymicrobial synergy.

描述（由申请人提供）：大多数细菌发病机理研究都集中在单养殖感染上；但是，很明显，许多细菌感染不仅是与单个物种定殖的结果，而且还来自多数菌群群落的作用。多人感染中的微生物通常显示出协同的相互作用，从而在感染部位增强定殖，持久性和抗生素耐药性。这种相互作用在多菌粒软组织感染中尤其注意到烧伤和慢性伤口，尽管控制这些协同相互作用的分子过程通常尚不清楚。研究阐明了有助于增强体内持续性的多数型相互作用所涉及的遗传途径对于对协同的全面理解和开发治疗多种特征感染的治疗剂的必要第一步至关重要。该研究计划的总体目标是从基因组的角度检查机会病原体之间的相互作用如何影响急性和慢性伤口感染的体内持久性。为了实现这一目标，将采用高通量基因组学技术来识别在多生物机构协同期间需要和表达的微生物基因。