Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC) Supplement

斯坦福-SLAC CryoET 标本制备服务中心 (SCSC) 补充资料

基本信息

批准号：
10265915
负责人：
Wah Chiu
金额：
$ 37.73万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-16 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10265915
关键词：
Academia Advisory Committees Area Award Biocompatible Materials Biomedical Research Budgets Cells Cellular Structures Collaborations Communication Communities Complex Consult Cryo-electron tomography Cryoelectron Microscopy Data Collection Educational Curriculum Educational workshop Electron Microscope Equipment European Evaluation Fluorescence Microscopy Freezing Funding Goals Grant Hour Hydration status In Situ Industry Infrastructure Institution Investigation Ions Laboratories Lifting Macromolecular Complexes Manufacturer Name Methods Molecular Structure Organelles Performance Preparation Procedures Process Protocols documentation Request for Applications Research Research Personnel Resolution Resource Allocation Sampling Scanning Scanning Electron Microscopy Schedule Scientist Selection Criteria Services Slice Specimen Subcellular structure Techniques Technology Thick Tissue Sample Tissues Titan Training Training Activity Work data management experience experimental study imaging modality instrument instrumentation interest member operation pressure programs prototype response structural biology tool

项目摘要

Project Summary Upgrade of Aquilos to Aquilos 2 cryo-focused ion beam scanning electron microscope. In our original proposed budget, we requested ~$1M for a focused ion beam instrument. We did not specify the manufacturer of such instrument (i.e. Thermo Fisher or Zeiss). The recent announcement from Thermo Fisher of the Aquilos 2 (~$1.38M), offers multiple exciting features. It has the capabilities of performing lift-out, slice and view and automated milling with 20+ lamellae in 13 hours of operation. These results suggest that cryoFIB can become an automated procedure on very thick, high-pressure vitrified samples. This would be a highly sought-after capability of our Service Center, because we anticipate that there will be a significant demand if we can begin to work with tissues tens to hundreds of microns in thickness. These performance specifications and the results shown by Thermo Fisher are exciting. If funded, we will also investigate whether Zeiss can offer a similar or superior instrument before we make a purchase decision. Certainly, we will consult with our European colleagues who have the prototypes of the new instrument in terms of its robustness as a user facility as in our Center.

项目概要 Aquilos 升级为 Aquilos 2 冷冻聚焦离子束扫描电子显微镜。在我们原来的在拟议的预算中，我们要求约 100 万美元购买聚焦离子束仪器。我们没有指定此类仪器的制造商（即 Thermo Fisher 或 Zeiss）。 Thermo 最新公告 Fisher of the Aquilos 2（约 138 万美元）提供了多种令人兴奋的功能。它具有执行的能力在 13 小时的操作时间内对 20 多个薄片进行提升、切片和查看以及自动铣削。这些结果建议 CryoFIB 可以成为处理非常厚的高压玻璃化样品的自动化程序。这将是我们服务中心备受追捧的一项功能，因为我们预计如果我们能够开始使用数十至数百微米的组织，这将是一个巨大的需求厚度。 Thermo Fisher 展示的这些性能规格和结果令人兴奋。如果如果获得资助，我们还将在制作之前调查蔡司是否可以提供类似或更好的仪器购买决定。当然，我们会咨询拥有原型的欧洲同事新仪器作为我们中心的用户设施的稳健性。