Structural Analysis of a Conserved Alphaproteobacterial Stress Response Pathway

保守的α变形菌应激反应途径的结构分析

基本信息

批准号：
8796631
负责人：
Jonathan W Willett
金额：
$ 5.42万
依托单位：
UNIVERSITY OF CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-02-01 至 2017-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8796631
关键词：
Affect Affinity Alphaproteobacteria Bacteria Binding Binding Sites Biochemical Biological Assay Biological Models Biophysics C-terminal Caulobacter Caulobacter crescentus Cells Chemicals Crystallization Data Discipline Environment Faculty Family Fluorescence Resonance Energy Transfer Fluorides Foundations Gene Expression Genetic Genetic Transcription Goals Growth Health Hybrids In Vitro Infection Measures Methods Microbial Biofilms Microbial Physiology Modeling Molecular Molecular Conformation Mutagenesis N-terminal NMR Spectroscopy Oxidative Stress Pathogenesis Pathway interactions Phenotype Phosphorylation Phosphotransferases Play Positioning Attribute Property Protein Region Proteins Proteobacteria Regulation Reporter Research Roentgen Rays Role Sigma Factor Signal Pathway Signal Transduction Signaling Protein Stress Structure Surface Plasmon Resonance Symbiosis System Techniques Testing Transcriptional Regulation Virulence Factors X-Ray Crystallography base biological adaptation to stress biophysical analysis biophysical techniques genetic regulatory protein high risk in vivo inorganic phosphate interdisciplinary approach microbial mutant novel pathogen polypeptide post-doctoral training protein structure research study response stability testing stressor structural biology yeast two hybrid system

项目摘要

DESCRIPTION (provided by applicant): Bacterial cells utilize both two-component signaling proteins and alternative sigma (σ) factors to modulate transcription in response to environmental stress. These environmental stress regulatory proteins are known to function as essential virulence factors in numerous bacterial pathogens. Bacterial stress responses play a fundamental role in bacterial growth, biofilm formation, pathogenesis and symbiosis. My proposed structural studies on the Caulobacter crescentus general stress signaling pathway, which integrates feature of two-component signaling and alternative s regulation, will significantly enhance our understanding of the molecular and structural basis of stress adaptation in the alphaproteobacteria. The Crosson lab has a successful track record utilizing techniques from a wide range of disciplines to pursue questions focused on molecular microbial physiology. Their broad interdisciplinary approaches promise to provide a more complete understanding of how bacterial cells can perceive and adapt to changes within their environment. My postdoctoral training in the Crosson Lab, focused on molecular biophysics and structural biology of the PhyR, σT and NepR proteins, will facilitate my successful transition into an independent faculty position, where my research will center on molecular aspects of microbial signaling.

描述（由申请人提供）：细菌细胞利用双组分信号蛋白和替代西格玛（σ）因子来调节转录以响应环境应激，已知这些环境应激调节蛋白在许多细菌病原体中充当必需毒力因子。细菌应激反应在细菌生长、生物膜形成、发病机制和共生中发挥着重要作用。我提出的新月柄杆菌一般应激信号通路的结构研究，整合了细菌的特征。双组分信号传导和替代调节，将显着增强我们对α变形菌应激适应的分子和结构基础的理解，Crosson 实验室在利用广泛学科的技术来解决分子微生物问题方面拥有成功的记录。他们广泛的跨学科方法有望提供对细菌细胞如何感知和适应环境变化的更全面的了解。 σT 和 NepR 蛋白将促进我成功过渡到独立教职职位，我的研究将集中在微生物信号传导的分子方面。