TAS::75 0862::TAS

塔斯马尼亚州::75 0862::塔斯马尼亚州

• 批准号: 8164000
• 负责人: MARTIN D. CHAPMAN
• 金额: $ 14.93万
• 依托单位: INDOOR BIOTECHNOLOGIES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-28 至 2011-09-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8164000
• 关键词: Alabama; Allergens; Allergic Disease; Alternaria; Antibodies; Antigens; Arts; Aspergillus; Aspergillus fumigatus; Asthma; Biological Assay; Biological Markers; Biotechnology; Birds; Calibration; Canada; Centers for Disease Control and Prevention (U.S.); Chronic Fatigue Syndrome; Cities; Client; DNA; Detection; Development; Dust; Environmental Exposure; Epidemiologic Studies; Evaluation; Exposure to; Extrinsic asthma; Government Agencies; Health; House Dust; Housing; Immune response; Indoor Air Quality; Industry; Institution; Investigation; Kansas; Lung diseases; Measures; Methods; Migraine; Missouri; Molds; Monoclonal Antibodies; National Institute of Environmental Health Sciences; Penicillium; Phase; Polystyrenes; Reproducibility; Research; Role; Sales; Sampling; Sensitivity and Specificity; Services; Sinusitis; Stachybotrys; System; Technology; Testing; Validation; Water; cross reactivity; innovation; instrumentation; interest; phase 1 study; phase 2 study; population survey

Molds cause asthma and allergic respiratory diseases and are associated with other illnesses such as sinusitis, chronic fatigue syndrome, lethargy, or migraines. Mold occurs in damp or water damaged housing. The relationships between mold exposure and health effects are poorly understood. The aim of this project is to develop a multiplex array for mold biomarkers that will measure 4-7 biomarkers, simultaneously, from molds associated with asthma or that are found in water damaged buildings. The Technical Objectives are i) to develop a fluorescent Multiplex Array for Mold Biomarkers (MAMB) on the xMAP system to measure allergens/antigens from Alternaria, Aspergillus fumigatus, A. versicolor, Stachybotrys and Penicillium spp.; ii) to determine the sensitivity, specificity, reproducibility of the MAMB; and iii) to use the MAMB to measure mold exposure in dust samples from environmental studies of damp housing in the U.S. and Canada. The MAMB will use monoclonal antibody pairs for allergen capture and detection and a standard of purified biomarkers for calibration purposes. Current methods for mold testing, such as mold counts or DNA analyses, do not measure specific biomarkers. The MAMB will apply innovative, state-of-the art array technology to assess exposure to specific mold antigens, some of which are known to cause deleterious immune responses. The array for up to 7 biomarkers is expandable to include other mold antigens, as part of Phase II studies. The MAMB will provide quantiative measures of mold biomarker exposure in a single test and will facilitate large scale epidemiologic studies and population surveys on the health effects of environmental exposure to molds.

霉菌会引起哮喘和过敏性呼吸道疾病，并与其他疾病有关，例如 鼻窦炎、慢性疲劳综合症、嗜睡或偏头痛。霉菌发生在潮湿或水损坏的外壳中。 人们对霉菌暴露与健康影响之间的关系知之甚少。该项目的目的 的目标是开发一种霉菌生物标志物多重阵列，可同时测量 4-7 个生物标志物， 与哮喘相关的霉菌或在被水损坏的建筑物中发现的霉菌。技术目标是 i) 在 xMAP 系统上开发霉菌生物标记物荧光多重阵列 (MAMB) 来测量 来自链格孢属、烟曲霉、杂色曲霉、葡萄穗霉属和青霉属的过敏原/抗原；二） 确定 MAMB 的敏感性、特异性和重现性； iii) 使用 MAMB 来测量 美国和加拿大潮湿房屋环境研究中灰尘样本中的霉菌暴露情况。这 MAMB 将使用单克隆抗体对进行过敏原捕获和检测以及纯化的标准品 用于校准目的的生物标志物。当前的霉菌测试方法，例如霉菌计数或 DNA 分析，不测量特定的生物标志物。 MAMB 将采用创新、最先进的阵列 评估接触特定霉菌抗原的技术，其中一些已知会导致有害的霉菌抗原 免疫反应。该阵列可容纳多达 7 种生物标志物，可扩展至包括其他霉菌抗原，作为 第二阶段研究。 MAMB 将在一次测试中提供霉菌生物标志物暴露的定量测量 并将促进关于健康影响的大规模流行病学研究和人口调查 环境暴露于霉菌。