FACS Core Facility

FACS 核心设施

• 批准号: 8158372
• 负责人: William Telford
• 金额: $ 71.07万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8158372
• 关键词: CCR; Core Facility; Data Analyses; Data Collection; Dermatology; Environment; Equipment; Laser Scanning Cytometry; Length

The Core provides a wide variety of state-of-the-art flow and image cytometry instrumentation. Full support is provided to users of this instrumentation, including initial planning of experiments, training on the appropriate instrumentation, assistance in data collection, subsequent data analysis, and final presentation of results. Investigators are trained in the use of most analysis-only instrumentation, after which they can use it independently with minimal supervision. For fluorescence-activated cell sorting, a trained operator performs the sort with the investigator present. Specialized instrumentation (including laser scanning cytometry and full spectrum cytometry) is also carried out with considerable assistance from the Core staff. The Core provides an active educational environment for ETIB investigators, including regularly scheduled workshops on new assay and instrument technologies, and regular presentations at Branch and laboratory seminars. The Core also provides instructional seminars for all other Branches covered under their services. Dr. Telford is the co-coordinator of the NIH Flow Cytometry Interest Group, a NIH-registered scientific interest group (http://www.nih.gov/sigs). The Core maintains an informational website describing both Core services and policies, and internal research projects in the Core (http://home.ncifcrf.gov/ccr/flowcore/index.htm). The Core also maintains an extensive network of training and collaborative contacts outside the NCI. Dr. Telford is a sustaining faculty member of the National Flow Cytometry Resource Summer Cytometry School, a yearly intensive workshop on cytometry theory and technique. This program is sponsored by a NCRR P41 grant to Los Alamos National Labs, and is the only national cytometry training school of its kind. Dr. Telford has given the Apoptosis lecture and run the Apoptosis hands-on laboratory from 2004 to 2008. Dr. Telford is also a sustaining faculty member in the Indo-American Flow Cytometry Workshops, another yearly workshop aimed at training young Indian students and investigators in flow cytometry. This workshop is partly sponsored by the NCI, and was held in Mumbai in 2004, Trivandrum in 2006 and New Delhi in 2007. Dr. Telford gives the Flow Cytometry of Stem Cells seminar at these meetings, and manages wet workshops on the same topic. Dr. Telford has also established a sister flow cytometry laboratory at the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry in Moscow, funded by multiple U.S. Department of Health and Human Services grants funded through the U.S. Department of State and the Biotechnology Engagement Program (BTEP), and equipped with flow cytometers through donations of used equipment from the NCI. The Core hosts students and scientists for short-term training visits from laboratories around the world, including Russia, Kazakhstan, Azerbaijan, Singapore, Saudi Arabia and Turkey. The Core also maintains a small internal research and development program, aimed at developing new assay and detection technologies, and at instrument development. Stem cell analysis, apoptosis detection and flow cytometric telomere length measurement . The Core has a strong on-going program for integration of new laser technology into existing instrumentation, including solid-state diode and diode-pumped (DPSS) laser technology, fiber lasers, and non-linear supercontinuum laser technology. This technology allows the use of virtually any fluorescent probe for flow cytometry, and is made available to investigators throughout NIH.

核心提供了各种各样的最新流量和图像细胞仪仪器。向该仪器的用户提供全力支持，包括实验的初步计划，适当的仪器培训，数据收集的帮助，随后的数据分析以及结果的最终表示。研究人员接受了大多数仅分析仪器的使用，然后他们可以在最小的监督下独立使用它。对于荧光激活的细胞分选，训练有素的操作员与存在的研究者一起执行类似。在核心人员的大量帮助下，还进行了专门的仪器（包括激光扫描细胞仪和全光谱细胞仪）。该核心为ETIB研究人员提供了积极的教育环境，包括定期安排的有关新测定和仪器技术的研讨会，以及分支机构和实验室研讨会的定期演讲。核心还为所有其他分支机构提供教学研讨会。 Telford博士是NIH流式细胞仪兴趣组的协调员，NIH注册的科学兴趣组（http://www.nih.gov/sigs）。核心维护一个信息网站，描述了核心服务和策略，以及核心内部研究项目（http://home.ncifcrf.gov/ccr/ccr/flowcore/index.htm）。核心还保持了NCI以外的广泛培训和协作联系网络。 Telford博士是国家流式细胞仪资源夏季细胞仪学校的维持教职员工，这是一个关于细胞仪理论和技术的年度密集研讨会。该计划是由洛斯阿拉莫斯国家实验室（Los Alamos National Labs）的NCRR P41赠款赞助的，并且是同类国家的唯一一家国家细胞术培训学校。 Telford博士从2004年到2008年进行了凋亡讲座，并进行了动手实验室的凋亡。Telford博士也是印度美式流式细胞仪讲习班的维持教师，这是另一个年度研讨会，旨在培训流式细胞仪中的年轻印度学生和研究人员。该研讨会部分由NCI赞助，并于2004年在孟买举行，Trivandrum于2006年和2007年的新德里举行。Telford博士在这些会议上进行了干细胞研讨会的流式细胞仪，并在同一主题上管理湿工作室。 Telford博士还在莫斯科的Shemyakin和Ovchinnikov生物有机化学研究所建立了一个姊妹流式细胞仪实验室，该实验室由多个美国卫生和公共服务部资助，由美国国务院和生物技术部和生物技术部门互动计划（BTEP）（BTEP）提供资金，并配备了流式的电池仪。核心向学生和科学家接待了全球实验室的短期培训访问，包括俄罗斯，哈萨克斯坦，阿塞拜疆，新加坡，沙特阿拉伯和土耳其。该核心还保持了一个小型的内部研发计划，旨在开发新的测定和检测技术以及仪器开发。干细胞分析，凋亡检测和流式细胞仪端粒长度测量。该核心具有将新激光技术集成到现有仪器中的强大持续计划，包括固态二极管和二极管（DPSS）激光技术，光纤激光器和非线性超级固有激光器技术。 该技术允许使用几乎所有荧光探针进行流式细胞仪，并可以向整个NIH的研究人员使用。