Transgenic Mouse Facility

转基因小鼠设施

• 批准号: 8008876
• 负责人: LARRY ARTHUR
• 金额: $ 84.55万
• 依托单位: LEIDOS BIOMEDICAL RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-26 至 2018-09-25
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8008876
• 关键词: Client; Consultations; DNA purification; Dot Immunoblotting; Laboratories; Microinjections; Mus; National Cancer Institute; Pattern; Proteins; RNA; Recombinant DNA; Research Personnel; Services; Southern Blotting; Transgenic Mice; Transgenic Mouse Facility; design; egg; gene function; in vivo; mouse model; transgene expression

The Transgenic Mouse Model (TMM) Laboratory at the National Cancer Institute ¿ Frederick provides assistance to investigators with a need to study in vivo gene function, by producing transgenic mice by pronuclear microinjection of fertilized mouse eggs. Although this service is customized to the needs of each client, the core of this effort includes purification of the DNA fragment to be microinjected and analysis of the resulting mice by Southern blot, PCR, or slot/dot blot. Consultations for the design of recombinant DNA constructs for the use in transgenic mouse studies are available. Characterization of the pattern of transgene expression at the RNA or protein level is also available. Transgenic mice are routinely produced in FVB/N and C57BL/6 backgrounds.

国家癌症研究所的转基因小鼠模型（TMM）实验室»弗雷德里克（Frederick）通过对受精小鼠卵的前核显微注射产生转基因小鼠，为研究人员提供了研究体内基因功能的帮助。尽管该服务是根据每个客户的需求定制的，但此工作的核心包括纯化要对其进行微注射的DNA片段以及通过Southern blot，pcr或插槽/点斑点对所得小鼠进行分析。可以为在转基因小鼠研究中使用重组DNA构建体的设计咨询。还可以提供RNA或蛋白质水平转化表达模式的表征。转基因小鼠通常在FVB/N和C57BL/6背景中产生。