High Frequency Acceleration to Restore Alveolar Bone

高频加速恢复牙槽骨

基本信息

批准号：
8978238
负责人：
Bryce Way
金额：
$ 22.41万
依托单位：
PROPEL ORTHODONTICS, LLC
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-09-01 至 2017-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8978238
关键词：
Acceleration Adverse effects Affect Alveolar Bone Loss American Animal Model Biological Preservation Bone Density Bone Resorption Bone Transplantation Clinical Dentistry Dentures Development Devices Edentulous Mouth Engineering Exposure to Frequencies Gene Expression Gene Proteins Healed Health Height Implant Implantation procedure Investigation Jaw Lead Legal patent Longitudinal Studies Mandible Manufacturer Name Mastication Maxillary Sinus Mechanical Stimulation Mechanics Medical Device Methods Microscopy Modality Modeling Molecular Morphology Mucous Membrane Muscle Names Nasal cavity Natural regeneration New York Oral cavity Orthodontic Orthodontics Osteoblasts Osteoclasts Osteogenesis Osteoporosis Outcome Pathologic Patients Pattern Periodontal Diseases Phase Population Procedures Prosthesis Quality of life Rattus Reconstructive Surgical Procedures Research Research Personnel Salivary Glands Site Skeleton Spectroscopy, Fourier Transform Infrared Staining method Stains Structure Technology Testing Therapeutic Effect Time Tissues Tooth Extraction Tooth Loss Tooth structure Universities Weight-Bearing state Width activity marker aged alveolar bone bone bone healing bone loss college cost effective density effective therapy graft healing healing improved innovation microCT osteogenic phase 1 study protein expression public health relevance regenerative response

项目摘要

DESCRIPTION (provided by applicant): The health of the oral cavity depends on the alveolar bone, which may be compromised by: loss of morphology (decrease in height/width) or decreased density. Treatments for alveolar bone loss are the application of bone grafts and placement of implants. Only a small percentage of population is able to afford treatment. This suggests the majority of the population is exposed to the side effects of progressive alveolar bone loss with significant impact in the quality of life. The current project will investigate the effect of high frequency acceleration (HFA) on alveolar bone in animal models of pathological conditions. Specific Aim 1: To determine the preservation effect of HFA on alveolar bone morphology. An extraction model will be used that mimics the significant decrease in width and height alveolar bone in a short period of time. The company will engineer a device to will study the effect of HFA on the quantity and quality of alveolar bone of rats after extraction at two phases: the bone healing phase and the post-healing phase when the majority of bone loss occurs. Alveolar bone density and morphology will be evaluated by microCT. Osteogenic effect of HFA will be examined using fluorescent and FTIR microspectroscopy, while anti-resorptive effect of HFA will be investigated by osteoclasts immunohistochemical staining (TRAP and Catapsin K). Activation of osteoblasts and osteoclasts markers will be further analyzed at protein and gene expression levels, and by detailed immunohistochemical analysis. Specific Aim 2: To determine the regenerative effects of HFA on alveolar bone density. To study the therapeutic effect of HFA on alveolar bone density, an osteoporosis model will be used that shows a significant decrease in alveolar bone density in a short period of time. Osteogenic and anti-resorptive effects of HFA will be studied in the short and long-term studies. Bone response will be evaluated at the tissue level by microCT, FTIR microspectroscopy, fluorescent microscopy and immunohistochemical analysis, and at the molecular level by analyzing gene expression and protein activity of markers of osteoblast and osteoclast activity. This investigation could have an enormous impact in the field of clinical dentistry and on quality of lie for millions of edentulous patients. Not only it will establish a non-invasive approach to preserve healthy bone in the jaws, but also it will offer an extremely effective method to recover bone density that has been lost to different pathological conditions. In addition, this method may be used as adjunct therapy to implant integration or graft healing, or any other procedure where a strong osteogenic response is needed.

描述：口腔的健康取决于高度/宽度的肺部骨骼易于骨骼或降低的骨骼骨骼损失。在生活质量中。在短时间内，高度肺泡骨将在两个阶段研究HFA对肺泡骨的四分之一的影响：出现2：确定骨骼密度，骨质疏松模型将在短期内通过荧光镜，在短时间内tos to。和免疫组织化学分析Zing基因表达活性细胞和破骨细胞的作用。颌骨中的健康骨骼将提供极端的恢复骨密度，此外，该方法可以用作植入式疗法或移植物愈合，在需要强大的妇产科反应。