Structural Biology of Influenza Epitopes

流感表位的结构生物学

基本信息

批准号：
8946542
负责人：
Audray Harris
金额：
$ 119.09万
依托单位：
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8946542
关键词：
Address Affect Animal Viruses Animals Antibodies Antigens Architecture Area Attenuated B-Lymphocytes Binding Biochemical Coin Communicable Diseases Complex Drug Formulations Electron Microscopy Epitopes Evaluation Failure Future Geometry Goals Health Hemagglutinin Human Immune response Immune system Immunity Influenza Influenza A Virus, H1N1 Subtype Influenza A Virus, H7N9 Subtype Influenza Hemagglutinin Investigation Life Membrane Glycoproteins Mission Molecular Molecular Models Nature Pharmaceutical Preparations Phase Population Proteins Public Health Recombinants Research Scaffolding Protein Structure Subunit Vaccines T-Lymphocyte T-Lymphocyte Epitopes Testing Vaccine Design Vaccines Viral Vaccines Virus Virus-like particle arm base design immunogenic influenza outbreak influenza virus vaccine influenzavirus innovation killings molecular modeling nano nanoimaging nanoparticle neutralizing antibody novel pandemic disease scaffold stem structural biology success transmission process

项目摘要

Although seasonal vaccines are available, the influenza virus continues to be a major health burden due to its capacities to alter the antigenic epitopes of the main surface glycoprotein hemagglutinin, and its zoonotic nature, permitting transmission of novel animal viruses to humans to which the human population has no preexisting immunity. Although conserved epitopes have been identified among hemagglutinin subtypes, there is a fundamental gap in understanding and correlating the display of influenza hemagglutinin epitopes on nano-platforms with immunogenic success or failure in terms of developing broader spectrum seasonal vaccines and a universal influenza vaccine. Lack of such information represents important problems and until they are addressed optimal display of conserved epitopes cannot be understood in molecular details. In FY 2014, we have continued characterizing influenza viruses and epitopes on influenza hemagglutinin and have established significant milestones both in designing and structurally imaging nano-platforms presenting conserved regions of hemagglutinin. These results are significant and relevant to public health because it is expected to expand understanding of the structure and epitope disposition of influenza epitopes on nano-platforms that will aid immunogen design for universal influenza vaccines.

尽管可以使用季节性疫苗，但由于其能力改变了主要表面糖蛋白凝血素血凝蛋白的抗原表位，但流感病毒仍然是重大的健康负担，它允许将新型动物病毒传播给人类的人群具有免疫性的免疫力。尽管在血凝素亚型中已经鉴定出了保守的表位，但在理解和相关的纳米平台上的流感hemagglutinin epistopes与免疫原性的成功或免疫性季节性季节性疫苗和通用型甲壳虫的影响方面存在基本差距。缺乏此类信息代表了重要的问题，直到解决这些信息的最佳表现为止，因此无法通过分子细节来理解保守的表位。在2014财年，我们继续表征流感病毒和对流感血凝素的表位，并在设计和结构成像的纳米平台上建立了重要的里程碑，这些纳米平台呈现出血凝素的保守区域。这些结果与公共卫生相关，因为它有望扩大对纳米平台上流感表位的结构和表位处置的理解，这些纳米平台将有助于免疫原料设计，以实现通用流感疫苗的免疫原。