Tissue Core

组织核心

• 批准号: 8733433
• 负责人: LAURA Z RASSENTI
• 金额: $ 53.87万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8733433
• 关键词: Antigens; B Cell Proliferation; B-Lymphocytes; Basic Science; Biochemistry; Biological Assay; Biology; Cell Cycle Kinetics; Cell surface; Cells; Chronic Lymphocytic Leukemia; Clinical; Clinical Data; Clinical Protocols; Clinical Research; Clinical Trials; Clonal Expansion; Confidentiality; Confidentiality of Patient Information; Cytogenetics; Data; Data Quality; Data Set; Deuterium Oxide; Development; Diagnosis; Disease; Disease Progression; Enrollment; Ensure; Equipment; Ethics; Evaluation; Freezing; Genes; Genetic; Genotype; Health Insurance Portability and Accountability Act; IGH@ gene cluster; Immunologics; Immunophenotyping; Indolent; Informed Consent; Instruction; Kinetics; Label; Legal; Longitudinal Studies; Management Information Systems; Membrane; Outcome; Participant; Patients; Phenotype; Ploidies; Privacy; Procedures; Process; Progressive Disease; Records; Regulation; Relative (related person); Research; Research Design; Research Infrastructure; Research Personnel; Research Project Grants; Resource Sharing; Resources; Rest; Role; Sampling; Services; Site; Surface; Surface Antigens; Technology; Testing; Tissue Banking; Tissue Banks; Tissues; Translating; Validation; base; biobank; clinical practice; clinical research site; cohort; improved; leukemia; member; novel; outcome forecast; prognostic; repository; trafficking

The Tissue Core (CORE C) mandate is to provide a national resource that consists of a dataset of matched samples of frozen cells with corresponding pathological and clinical data. The Tissue Core is an integral and essential component of this proposal since it is the central repository for all samples. It maintains accurate records of the trafficking of all samples and facilitates the uniform sharing of information between all CRC projects. In addition, it facilitates and expedites the research of each CRC project and clinical trials by performing centralized standardized assays and distributing characterized samples to all members of this proposal. This Core ensures the long-term physical integrity of the biospecimen and maintains the privacy and confidentiality of the research participant. The first aim of this Core is to serve as the central biorepository for all CLL samples diagnosed and collected from the participating clinical sites. This enables the uniform processing, storage, and distribution of all CLL samples used by the investigators of this proposal. The second aim is to perform a basic set of standardized assays on all viably stored CLL samples from each patient upon initial receipt, and as required by the clinical protocols. The third aim is to acquire serial samples during the disease progression of all CLL patients to enable longitudinal studies on the disease progression. CRC investigators can segregate cases that have indolent and non-progressive disease from those who have progressive disease or from those who have a change in the kinetics of disease progression. The fourth aim, a multi-site service, is to organize and assure accurate scoring and high quality data entry associated with FISH for CLL among all CRC sites. This service provides reliable cytogenetic data for clinical trials and research correlations. The fifth aim is to characterize CLL samples based on their cellular kinetics. As an adjunct to these studies, the expressed immunoglobulin heavy chain genes (IGHV) about the leukemic clones can then be correlated to cellular kinetics to study the role for (auto) antigen drive in the clonal expansion. Lastly, the sixth aim is to manage the distribution and tracking of specific characterized samples as requested for hypothesis-driven studies of this proposal.

组织核心（核心C）任务是提供由匹配数据集组成的国家资源 带有相应病理和临床数据的冷冻细胞样品。组织芯是一个不可或缺的 该提案的重要组成部分，因为它是所有样品的中央存储库。它保持准确 所有样品贩运的记录，并促进了所有CRC之间的信息统一共享 项目。此外，它促进并加快了每个CRC项目和临床试验的研究 对所有成员进行集中的标准化测定和分发样本的特征 提议。该核心确保了生物测量的长期物理完整性并保持隐私 和研究参与者的机密性。该核心的第一个目的是作为中心 从参与临床部位诊断并收集的所有CLL样品的生物座席。这可以 研究人员使用的所有CLL样品的均匀处理，存储和分布 提议。第二个目的是在所有储存的CLL样品上执行一组基本的标准化测定 初次收到的每个患者，以及临床方案的要求。第三个目的是获取 所有CLL患者疾病进展过程中的连续样本能够纵向研究 疾病进展。 CRC调查人员可以隔离具有懒惰和非促进性​​的病例 从患有进行性疾病的人或那些有改变动力学的人的疾病 疾病进展。第四个目标是多站点的服务，是组织和确保准确的评分和 所有CRC站点中与CLL的鱼类相关的高质量数据输入。该服务提供可靠的 用于临床试验和研究相关性的细胞遗传学数据。第五目的是表征CLL样品 基于它们的细胞动力学。作为这些研究的辅助，表达的免疫球蛋白重链 然后，关于白血病克隆的基因（IGHV）可以与细胞动力学相关，以研究（自动）的作用 克隆膨胀中的抗原驱动。最后，第六个目标是管理的分布和跟踪 根据要求对该提案进行假设驱动的研究的特定表征样品。