Acquisition of a Hybrid Quadrupole Time of Flight LC-MS/MS System for the Cornell

为康奈尔大学采购混合四极杆飞行时间 LC-MS/MS 系统

• 批准号: 8639277
• 负责人: Sheng Zhang
• 金额: $ 60万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8639277
• 关键词: Biological; Biotechnology; Complex; Core Facility; Funding; Future; Growth; Hand; High Pressure Liquid Chromatography; Hybrids; Mass Spectrum Analysis; Measurement; Peptides; Performance; Post-Translational Protein Processing; Process; Proteomics; Research; Research Personnel; Research Project Grants; Resolution; Resources; Running; Sampling; Scanning; Services; Speed; System; Technology; Time; United States National Institutes of Health; Universities; base; cost effective; experience; flexibility; improved; instrument; instrumentation; mass spectrometer; nano

DESCRIPTION (provided by applicant): New mass spectrometry (MS) technologies present an exceptional opportunity for creative applications with potential for breakthrough discoveries. We request funding a high performance Q-TOF mass spectrometer (AB SCIEX TripleTOF" 5600+), interfaced with an ultra-high performance liquid chromatography system (chip-based ekspert" nanoLC 425). This state-of-the-art system will be located in the Proteomics and Mass Spectrometry Facility of the Cornell University Biotechnology Resource Center (BRC). Implementation of the instrument in the BRC as a university-wide shared research resource will enable cost effective access to this vital technology for a broad range of NIH-funded research. There is a growing demand for proteomics and mass spectrometry services at Cornell, as shown by the rapid and continuing growth in the number of facility users and samples processed by the facility over the past nine years. The proposed integrated instrument system will expand the capacity and capabilities of the facility but not duplicate the existing instrumentation at the core facility, which is currently utilized at near full capacity. The need fr increased performance and capacity is particularly critical with respect to applications involving analysis of complex samples which require enhanced scan speed, high sensitivity and resolution, and highly accurate mass measurements. The integrated system requested will provide improved dynamic range and will enable confident identification of peptides from low abundance proteins and of post-translational modifications (PTMs) in complex samples. The TripleTOF 5600+ will significantly enhance the facility's capability to support proteomics discovery studies in qualitative exploration as well as high throughput profiling and high-resolution quantification studies involving complex biological samples. The ekspert" nanoLC 425 with the cHiPLC(R) system is an integrated chip-based UHPLC that provides broad 2D LC flexibility and continuous direct nano flow. It has flexibility in workflow selection using chip-baed columns and offers a broad range of flow rates for both global discovery and higher throughput targeted quantitation. The nanoLC 425 system will provide automated sample handing and high resolution peptide separation prior to MS, which will enable continuous 24/7 analysis of very complex samples. The BRC has extensive experience in running LC MS instrument systems and providing MS applications as core facility services. The proposed instrument system will be readily integrated into the facility's established proteomics workflows and will significantly expand the facility's quantitative proteomics pipeline. Placement of the instrument system in the BRC will enable a diverse group of NIH funded investigators to undertake research projects that would not otherwise be economically or technically feasible. This proposal describes 39 projects from 16 NIH funded investigator groups that will utilize the proposed system. The requested instrument will benefit a wide range of current and future NIH-funded projects.

描述（由申请人提供）：新的质谱法（MS）技术为具有突破性发现的创造性应用提供了出色的机会。我们要求为高性能质谱仪（AB Sciex Triplef“ 5600+）进行资金，并与超高性能液相色谱系统（基于芯片的EKSPERT“ Nanolc 425）相连）。该最先进的系统将位于康奈尔大学生物技术资源中心（BRC）的蛋白质组学和质谱设施中。该工具在BRC中的实施作为全大学共享的研究资源，将为广泛的NIH资助研究提供具有成本效益的这项重要技术的访问。康奈尔（Cornell）对蛋白质组学和质谱服务的需求不断增长，这表明，在过去九年中，该设施处理的设施使用者数量和样本的迅速增长和持续增长所表明。拟议的综合仪器系统将扩大设施的能力和能力，但不会复制核心设施的现有仪器，该工具目前可全额使用。对于涉及需要提高扫描速度，高灵敏度和分辨率以及高度准确质量测量值的复杂样品分析的应用，需要提高性能和能力尤为重要。所请求的集成系统将提供改进的动态范围，并能够自信地识别复杂样本中低丰度蛋白和翻译后修饰（PTMS）的肽。 5600+的三重态将显着增强该设施在定性探索中支持蛋白质组学发现研究的能力，以及涉及复杂生物样品的高吞吐量分析和高分辨率定量研究。带有Chiplc（R）系统的Ekspert“ Nanolc 425是基于芯片的集成UHPLC，可提供宽阔的2D LC灵活性和连续的直接纳米流动。它在工作流程中具有灵活性，使用芯片底线列进行了灵活的选择，并使用芯片底线提供了较高的流量，并为全球范围的发现和较高的跨性别定量提供了较高的流量。 MS将对非常复杂的样本进行连续的24/7分析。否则在经济上或技术上是不可行的。该提案描述了来自16个NIH资助的研究人员群体的39个项目，这些项目将利用拟议的系统。所请求的工具将使当前和未来的NIH资助项目受益。

项目成果

The canine activated platelet secretome (CAPS): A translational model of thrombin-evoked platelet activation response.

• DOI: 10.1002/rth2.12450
• 发表时间: 2021-01
• 期刊: Research and practice in thrombosis and haemostasis
• 影响因子: 4.6
• 作者: Cremer SE;Catalfamo JL;Goggs R;Seemann SE;Kristensen AT;Szklanna PB;Maguire PB;Brooks MB
• 通讯作者: Brooks MB

Secreted sphingomyelins modulate low mammary cancer incidence observed in certain mammals.

• DOI: 10.1038/s41598-020-77639-1
• 发表时间: 2020-11-25
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Ledet MM;Harman RM;Fan JC;Schmitt-Matzen E;Diaz-Rubio ME;Zhang S;Van de Walle GR
• 通讯作者: Van de Walle GR

Oxygen level regulates N-terminal translation elongation of selected proteins through deoxyhypusine hydroxylation.

• DOI: 10.1016/j.celrep.2022.110855
• 发表时间: 2022-05-24
• 期刊: CELL REPORTS
• 影响因子: 8.8
• 作者: Zhang, Yugang;Su, Dan;Zhu, Julia;Wang, Miao;Zhang, Yandong;Fu, Qin;Zhang, Sheng;Lin, Hening
• 通讯作者: Lin, Hening

MaXLinker: Proteome-wide Cross-link Identifications with High Specificity and Sensitivity.

MaXLinker：具有高特异性和灵敏度的全蛋白质组交联鉴定。

• DOI: 10.1074/mcp.tir119.001847
• 发表时间: 2020
• 期刊: Molecular & cellular proteomics : MCP
• 作者: Yugandhar,Kumar;Wang,Ting-Yi;Leung,AldenKing-Yung;Lanz,MichaelCharles;Motorykin,Ievgen;Liang,Jin;Shayhidin,ElnurElyar;Smolka,MarcusBustamante;Zhang,Sheng;Yu,Haiyuan
• 通讯作者: Yu,Haiyuan

Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare.

• DOI: 10.1038/s41598-023-31540-9
• 发表时间: 2023-03-21
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Diel de Amorim M;Dong L;Byron M;Foster RA;Klein C;Saleh M;Saleh T;Card C
• 通讯作者: Card C