Core E: Protein

核心E：蛋白质

基本信息

批准号：
9113177
负责人：
STEVEN C. ALMO
金额：
$ 76.29万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至 2017-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9113177
关键词：
Area Automation Biotechnology Calendar Chromatography Computers and Advanced Instrumentation Core Protein Crystallization Data Data Collection Databases Deposition Devices E protein Enzymes Gene Targeting Generations Incubators Intervention Laboratories Light Maintenance Medicine Methods Modeling National Institute of General Medical Sciences New York Pharmacologic Substance Photons Production Protein Structure Initiative Proteins Reagent Refractive Indices Research Robot Robotics Roentgen Rays Scientist Source Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Synchrotrons System Temperature United States National Institutes of Health X ray diffraction analysis X-Ray Diffraction beamline college cryogenics design detector light scattering mass spectrometer screening square foot structural biology structural genomics web site

项目摘要

Eli Lilly San Diego Center for Biotechnology in a purpose-built 12,000 square foot laboratory, equipped with all of the necessary instrumentation/computers, including 10 Temperature Controlled Shaker Incubators (capable of fermenting 180 liters per day), four 4-channel AKTAxpress Chromatography Robots plus 13 single channel AKTA Chromatography Systems, Applied Biosystems MALDI-TOF (Voyager DE-RP and Voyager DE-STR) Mass Spectrometers, Applied Biosystems ESI-Quadrupole Mass Spectrometer, ThermoFinnigan LCQ Deca XP Plus ESI-lontrap Mass Spectrometer for MS/MS analysis. Applied Biosystems Qtrap 4000 Mass Spectrometer, five Agilent 1100 HPLCs, Wyatt Minidawn Treso Multiangle Light Scattering and Optlab Refractive Index Detector System, an Aviv Model 215 CD Spectrometer, a Biacore T100, a Robbins Phoenix Crystallization Robot, and a Formulatrix Formulator robot for preparing optimization trays. The remaining 50% of protein crystallization will be conducted at AECOM in a purpose built, similarly equipped crystallization laboratory. X-ray diffraction screening and data collection will be conducted at the APS using Lilly's State-of-the-Art LRL-CAT insertion device beamline, which is equipped with a mosaic CCD area detector and cryogenic crystal handling/mounting robotics. During APS maintenance shutdowns (-25% of the calendar year), access to Beamline X29 at the National Synchrotron Light Source will permit uninterrupted access to X-ray data collection facilities and occasional opportunities for expert intervention for challenging diffraction data collection (as needed).

Eli Lilly San Diego Center for Biotechnology in a purpose-built 12,000 square foot laboratory, equipped with all of the necessary instrumentation/computers, including 10 Temperature Controlled Shaker Incubators (capable of fermenting 180 liters per day), four 4-channel AKTAxpress Chromatography Robots plus 13 single channel AKTA Chromatography Systems, Applied Biosystems MALDI-TOF (Voyager DE-RP and Voyager de-str）质谱仪，应用生物系统Esi-Quadrupole质谱仪，Thermofinnigan LCQ DECA XP Plus ESI-Lontrap质谱仪用于MS/MS分析。 Applied Biosystems QTRAP 4000质谱仪，五个Agilent 1100 HPLC，Wyatt Minidawn Treso Multiangle Multiangle Light Scracting和OptLab 折射率检测器系统，Aviv型号215 CD光谱仪，Biacore T100，Robbins Phoenix Crystallization Robot和用于准备优化托盘的公式配方器机器人。其余50％的蛋白质结晶将以建造的目的在AECOM上进行，类似地进行配备有结晶实验室。 X射线衍射筛选和数据收集将使用礼来公司的最先进的LRL-CAT插入装置光束线在APS上进行，该仪表配备了马赛克CCD区域探测器和低温晶体处理/安装机器人。在APS维护关闭（日历年的-25％）期间，访问横梁X29 国家同步器光源将允许不间断地访问X射线数据收集设施，并偶尔提供专家干预的机会，以挑战衍射数据收集（根据需要）。