Cell Culture Resource Core

细胞培养资源核心

• 批准号: 8806580
• 负责人: Yulia A Komarova
• 金额: $ 24.82万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8806580
• 关键词: Adenoviruses; B-Lymphocytes; Cell Culture Techniques; Core Facility; Endothelial Cells; Ensure; Genetic; Goals; Human; Intercellular adhesion molecule 1; Knockout Mice; Lung; MYLK gene; Methods; Mus; NADPH Oxidase; Permeability; Primary Cell Cultures; Principal Investigator; Protein Kinase C Alpha; Proto-Oncogene Proteins c-akt; Quality Control; Recombinants; Reproducibility; Research Personnel; Resources; Schedule; Services; Signal Transduction; Techniques; Transfection; Transgenic Organisms; lung vascular injury; meetings; mouse model; programs; research study; success

Core B is a central resource for primary endothelial cell cultures and adenoviruses available for each of the four projects. This core facility will provide services for the following: 1) characterization and culture of primary human lung microvascular endothelial cells; 2) isolation, characterization and culture of primary lung microvascular endothelial cells of mouse origin including those of different genetic mouse models and 3) amplification and purification of recombinant adenoviruses for the experiments proposed in the Projects as outiined in the Core description below. Human and mouse lung endothelial cell cultures are pivotal for the accomplishment of studies proposed in all four Projects. Specifically, Core B is critical for providing endothelial cells isolated from different genetic mouse models; e.g.,fiklGPF*'"transgenic and Cav-1, eNOS, AKT-1, Src, Nox2 and PAR-1 knockout mice for Project 1; eNOS, iNOS, NADPH oxidase {p47phox and Nox2) and PAR-1 knockout mice for Project 2; Trpc6, TRPC1 and TRPC4, Src, PKCα, PAR-1 and EC-MLCK knockout mice for Project 3 and pi 10γ, Src, Aktl, Cav-1, ICAM-1 mice for Project 4. In addition. Core B will provide amplification and purification of recombinant adenoviruses for all four Projects. The activities of this Core are essential for the success of the Program and successful completion of all aims in the four projects.

Core B是用于每种原发性内皮细胞培养物和腺病毒的主要资源 四个项目。该核心设施将为以下服务提供服务：1）主要的表征和文化 人肺微血管内皮细胞； 2）原发性肺的隔离，表征和培养 小鼠起源的微血管内皮细胞，包括不同遗传小鼠模型和3） 在项目中提出的实验的重组腺病毒的扩增和纯化为 在下面的核心描述中排除在外。人和小鼠肺内皮细胞培养物对 在所有四个项目中提出的研究成就。具体而言，核心B对于提供 从不同遗传小鼠模型分离的内皮细胞；例如，fiklgpf*'“转基因和cav-1，eNos， 项目1的AKT-1，SRC，NOX2和PAR-1淘汰小鼠； eNOS，iNOS，NADPH氧化酶{p47phox和nox2） 和项目2的PAR-1淘汰小鼠； TRPC6，TRPC1和TRPC4，SRC，PKCα，PAR-1和EC-MLCK 项目3和PI10γ，SRC，AKTL，CAV-1，ICAM-1小鼠的敲除小鼠。核心核心 为所有四个项目提供重组腺病毒的扩增和纯化。活动的活动 核心对于该计划的成功和成功完成四个项目的所有目标至关重要。