Cell Culture Resource Core

细胞培养资源核心

• 批准号: 8806580
• 负责人: Yulia A Komarova
• 金额: $ 24.82万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8806580
• 关键词: Adenoviruses; B-Lymphocytes; Cell Culture Techniques; Core Facility; Endothelial Cells; Ensure; Genetic; Goals; Human; Intercellular adhesion molecule 1; Knockout Mice; Lung; MYLK gene; Methods; Mus; NADPH Oxidase; Permeability; Primary Cell Cultures; Principal Investigator; Protein Kinase C Alpha; Proto-Oncogene Proteins c-akt; Quality Control; Recombinants; Reproducibility; Research Personnel; Resources; Schedule; Services; Signal Transduction; Techniques; Transfection; Transgenic Organisms; lung vascular injury; meetings; mouse model; programs; research study; success

Core B is a central resource for primary endothelial cell cultures and adenoviruses available for each of the four projects. This core facility will provide services for the following: 1) characterization and culture of primary human lung microvascular endothelial cells; 2) isolation, characterization and culture of primary lung microvascular endothelial cells of mouse origin including those of different genetic mouse models and 3) amplification and purification of recombinant adenoviruses for the experiments proposed in the Projects as outiined in the Core description below. Human and mouse lung endothelial cell cultures are pivotal for the accomplishment of studies proposed in all four Projects. Specifically, Core B is critical for providing endothelial cells isolated from different genetic mouse models; e.g.,fiklGPF*'"transgenic and Cav-1, eNOS, AKT-1, Src, Nox2 and PAR-1 knockout mice for Project 1; eNOS, iNOS, NADPH oxidase {p47phox and Nox2) and PAR-1 knockout mice for Project 2; Trpc6, TRPC1 and TRPC4, Src, PKCα, PAR-1 and EC-MLCK knockout mice for Project 3 and pi 10γ, Src, Aktl, Cav-1, ICAM-1 mice for Project 4. In addition. Core B will provide amplification and purification of recombinant adenoviruses for all four Projects. The activities of this Core are essential for the success of the Program and successful completion of all aims in the four projects.

核心B是主要的室内培养物和腺病毒的中心资源 四个项目。 人肺微血管宇宙细胞； 2）分离，特征和原发性肺部。 小鼠起源的微血管界面细胞包括包括不同遗传小鼠模型3） 实验重组腺病毒的扩增和纯化 人类和小鼠肺核心培养物对于关键的关键是关键的。 在所有四个项目中均支撑的研究。 内皮细胞从不同的遗传小鼠模型中分离出来； AKT-1，SRC，NOX2和PAR-1基因敲除小鼠，用于ENOS，INOS，NADPH氧化酶 项目2和PAR-1淘汰小鼠； 项目3和PI10γ，SRC，AKTL，CAV-1，ICAM-1小鼠的敲除小鼠。 为所有四个项目提供放大和净化的武器。 核心对于该计划的成功和成功的所有目标都至关重要。