Developing powerful daisy drive systems for the precise alteration of local populations

开发强大的雏菊驱动系统以精确改变当地人口

• 批准号: 10085329
• 负责人: Kevin Esvelt
• 金额: $ 16.29万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10085329
• 关键词: Agreement; Clustered Regularly Interspaced Short Palindromic Repeats; Communities; Culicidae; Dengue; Disease; Disease Resistance; Environment; Ethics; Individual; Infection; International; Intervention; Lyme Disease; Malaria; Mutation; Nature; Organism; Parasitic Diseases; Population; Prevention; Public Health; Schistosomiasis; Secure; System; Testing; Wild Animals; ZIKA; base; combat; cost; fitness; gene drive system; public health relevance; reverse genetics; vector; vector-borne; weapons

Project Summary and Relevance Gene drive systems could solve ecological problems by altering entire populations of wild organisms. However, the self-sustaining nature of current CRISPR-based drive systems makes them likely to spread to most local populations, and very possibly to other populations of that species elsewhere in the world. Without a means of safe testing or securing international agreement prior to deployment, it is unclear how these types of gene drive systems can be ethically released. This proposal seeks to develop “daisy drive” systems capable of precisely and economically altering local populations of wild organisms. Specifically, the project will develop ways of reducing the fitness cost of both alteration and suppression drive systems, precisely controlling the extent to which they will spread, and perfectly reversing genetic changes spread by other types of CRISPR-based drive systems. By providing a path to community-supported, diplomatically feasible, and economical deployment, this proposal will facilitate the prevention of numerous vector-borne and parasitic diseases by spreading disease resistance among vectors or directly suppressing populations of disease agents. We anticipate that daisy drive interventions will serve as powerful weapons suitable for combating malaria, schistosomiasis, dengue, Zika, Lyme disease, and many more.

项目摘要和相关性 基因驱动系统可以通过改变野生动物的整个种群来解决生态问题 然而，当前基于 CRISPR 的驱动系统的自我维持性质使它们成为可能。 可能传播到大多数当地种群，并且很可能传播到该物种的其他种群 在世界其他地方没有安全测试或确保国际协议的手段。 部署，目前尚不清楚如何以合乎道德的方式发布这些类型的基因驱动系统。 寻求开发“菊花驱动”系统，能够精确且经济地改变当地人口 具体来说，该项目将开发降低两者健康成本的方法。 改变和抑制驱动系统，精确控制它们传播的程度， 并完美逆转其他类型的基于 CRISPR 的驱动系统传播的遗传变化。 通过提供一条获得社区支持、外交上可行且经济的途径 部署后，该提案将有助于预防大量病媒传播和寄生物 通过在媒介之间传播抗病性或直接抑制疾病群体来预防疾病 我们预计雏菊驱动干预将成为适合的强大武器。 防治疟疾、血吸虫病、登革热、寨卡病、莱姆病等。