FUNCTIONAL GENOMICS CORE

功能基因组核心

基本信息

批准号：
8574512
负责人：
BRIAN Keith DIECKGRAEFE
金额：
$ 16.81万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8574512
关键词：
Address Apoptosis Area Binding Bioinformatics Biological Process Budgets Cells ChIP-on-chip Chemotaxis Clinical Collection Commit Communities Complex Core Facility DNA Microarray Chip DNA-Binding Proteins Databases Development Digestive System Disorders Disease Equipment Event Expressed Sequence Tags Funding Gastrointestinal tract structure Gene Expression Profiling Genes Genetic Transcription Genome Hepatic Human Human Genome Project Human Resources Inflammation Institution Investigation Investments Laboratories Libraries Ligands Logic Matrix Metalloproteinases Measures Methodology Methods MicroRNAs Molecular Profiling Monitor Morphology Mus National Institute of General Medical Sciences Oligonucleotides Pathology Physiological Processes Polysaccharides Process Protein Array Protein-Carbohydrate Interaction Rattus Research Research Personnel Resources Role Sampling Services Streptavidin Structure Technology Time Tissue Banking Tissue Banks Tissue Procurements Tissues Training Universities Viral Cancer Virus Diseases Washington Work angiogenesis arm base cDNA Arrays chromatin immunoprecipitation cost cost effective density design experience functional genomics functional group gastrointestinal genome sequencing instrumentation lipid metabolism programs promoter yeast genome

项目摘要

The Human Genome Project and the development of the expressed sequence tag (EST) clone collection and database have revolutionized gene expression analysis. Instead of measuring one or a few genes, parallel DNA microarrays are capable of simultaneously measuring expression of thousands of genes, providing a glimpse into the logic and functional grouping of gene programs encoded by our genome. The term functional genomics encompasses varied approaches that provide a more global investigation into the expression and role of groups of genes in a physiologic process than has been previously available. As such, this approach represents a fundamental shift from the traditional 'one gene at a time' approach to the study of basic biological processes. cDNA microarrays and oligonucleotide-based microarray methodologies have the potential to provide a new level of information about cell or tissue function not previously possible. However, these technologies require an investment in expensive equipment and highly trained, experienced technicians. Most laboratories cannot afford to commit the substantial technical resources and personnel to the development and ongoing refinement of these particularly complex experimental methods. It is more cost effective to concentrate this instrumentation and highly skilled technical support in a centrally managed facility, where they will be efficiently utilized and cost effective. The Functional Genomics Core Facility was designed to provide this critical core expertise to the digestive disease research community. The unique feature of the Functional Genomics Core Facility is the expertise it provides to participating investigators on the application of high density comprehensive arrays to study gastrointestinal and hepatic qene expression. In maintaining an independent microarray core, DDRCC research investigators are provided with access to the Agilent platforms at extremely low costs ($275/2 sample comparisons using the 44K gene arrays) and with much more rapid turn around time (<5 days) than other microarray facilities in the institution. The close proximity of the Functional Genomics Core to the DDRCC Morphology Core and the DDRCC Tissue Procurement Facility greatly facilitates the processing and analysis of clinical samples related to digestive disease disorders. The DDRCC Functional Genomics Core works closely with other microarray facilities at this institution that offer alternative commercial platforms, such as the Affymetrix platforms supported by the CTSA sponsored Translational Pathology and Tissue Banking Core, and the Illumina platforms supported by the Genome Sequencing Center of Washington University. Important for tight budgets, the cost of our services is less than half the cost of a single sample Affy array, and is about 20% less than the Illumina platform. Just as important, the Functional Genomics Core provides integrative and bioinformatic services.

人类基因组项目和表达序列标签（EST）克隆收集的发展数据库已经彻底改变了基因表达分析。而不是测量一个或几个基因，而是平行 DNA微阵列能够同时测量数千个基因的表达，从而提供了瞥见由我们的基因组编码的基因程序的逻辑和功能组。术语功能基因组学涵盖了各种方法，可提供对表达和表达和基因组在生理过程中的作用比以前可用。因此，这种方法代表了从传统的“一个基因”方法到基本生物学研究的基本转变过程。 cDNA微阵列和基于寡核苷酸的微阵列方法有可能提供有关以前无法使用的细胞或组织功能的新信息。但是，这些技术需要对昂贵的设备和经验丰富的经验丰富的技术人员进行投资。最多实验室无力将大量的技术资源和人员投入开发，这些特别复杂的实验方法的持续改进。集中精力更具成本效益这种仪器和高技能的技术支持在中央管理的设施中有效利用和成本效益。功能性基因组学核心设施旨在提供此功能消化系统疾病研究界的关键核心专业知识。功能基因组学核心设施的独特功能是它为参与提供的专业知识调查人员关于高密度综合阵列在研究胃肠道和肝的应用 Qene表达。在维持独立的微阵列核心时，提供了DDRCC研究研究者以极低的成本访问敏捷平台（使用44K基因进行$ 275/2的样品比较阵列），并且比该机构的其他微阵列设施更快地扭转时间（<5天）。功能基因组学核心与DDRCC形态核心和DDRCC组织的紧密接近采购设施极大地促进了与消化系统相关的临床样本的处理和分析疾病障碍。 DDRCC功能基因组学核心与其他微阵列设施紧密合作提供替代商业平台的机构，例如CTSA支持的Affymetrix平台赞助的翻译病理学和组织库核心，以及由华盛顿大学的基因组测序中心。对于预算紧张重要的是，我们服务的成本是不到单个样品和阵列的成本的一半，比Illumina平台少20％。就像重要的是，功能基因组学核心提供了综合和生物信息学服务。