Delipidated Adipose Grafts for Craniofacial Reconstruction

用于颅面重建的脱脂脂肪移植物

基本信息

批准号：
8106247
负责人：
Patrick S Cottler
金额：
$ 37.19万
依托单位：
LUNA INNOVATIONS, INC.
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-08-01 至 2013-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8106247
关键词：
Adipocytes Adipose tissue Allergic Reaction Animal Model Animals Apoptosis Appearance Architecture Autologous Back Biological Assay Bioreactors Carbon Dioxide Cell Count Cells Characteristics Clinical Cosmetics Data Defect Devices Dorsal Endothelial Cells Engraftment Environment Esthetics Evaluation Fascia Fatty acid glycerol esters Filler Flow Cytometry Future Goals Harvest Health Human Immunocompromised Host Implant In Vitro Lead Life Lipids Liquid substance Location Measurement Mechanics Medical Methodology Methods Modeling Morphology Mus NOD/SCID mouse Operating Rooms Operative Surgical Procedures Outcome Patients Pericytes Phase Phase I Clinical Trials Pliability Population Porifera Preparation Procedures Process Protocols documentation Qualifying Rattus Regimen Reproducibility Research Rodent Model Schedule Specimen Staining method Stains Sterility Stromal Cells Suction Lipectomy System Techniques Technology Texture Time Tissue Engineering Tissue Grafts Tissue Sample Tissue Viability Tissues Transplantation United States National Institutes of Health Weight Work Wound Healing base clinical efficacy clinically relevant commercialization conditioning craniofacial design efficacy testing implantation improved in vivo innovation meetings novel novel strategies operation physical property pre-clinical pressure programs prototype public health relevance reconstruction research and development research study soft tissue statistics success tissue culture tissue processing tissue reconstruction user-friendly

项目摘要

DESCRIPTION (provided by applicant): Many craniofacial surgical procedures involve both functional and aesthetic reconstruction for successful completion. The need for soft tissue fill for additional functionality and contouring is an aspect that can have lower priority during reconstructive craniofacial surgeries. Following the major tissue reconstruction, this secondary phase of the procedure is of critical importance to the long term outcome. The current methods of using autologous fat as a fill material, lead to unpredictable and non-reproducible end tissue volume and physical properties. These were key factors in Luna Innovations' Phase I research plan that demonstrated in vitro and in vivo feasibility of an innovative approach to process tissue to achieve a more effective and reproducible volumes and tissue viability during autologous free fat transplantation (AFT) and tissue engineering. The goal of the Phase II is to refine the preliminary results and develop a clinically relevant standard procedure to process a patient's adipose tissue mechanically and/or chemically to force out the lipids prior to transplanting, where the body will convert the tissue back to functional, healthy adipose. This technology has promise to improve the clinical outcomes of end volume and texture with increased predictability and reproducibility over currently used AFT practices. Ultimately, the clinical system will consist of a fully automated and programmable bioreactor to perform the tissue processing that will produce more effective, more predictable and more reproducible soft tissue reconstruction while eliminating any rejection and allergic reactions. PUBLIC HEALTH RELEVANCE: Many reconstructive challenges associated with craniofacial procedures are characterized by a lack of soft tissue 'fill' and/or symmetry. The goal of this Phase II proposal is validate a method for in vitro mechanical delipidation of adipose tissue in preparation for autologous free fat transplantations and design the necessary fully-automated bioreactor. The novel methodology will lead to a more effective and reproducible enhancement and augmentation. Once commercialized, this technology has great medical potential in reconstructive and cosmetic applications as well as wound healing.

描述（由申请人提供）：许多颅面外科手术都涉及功能和美学重建才能成功完成。在颅面重建手术中，需要软组织填充来实现额外的功能和轮廓，这一点可能具有较低的优先级。在主要组织重建之后，手术的第二阶段对于长期结果至关重要。目前使用自体脂肪作为填充材料的方法导致最终组织体积和物理特性不可预测且不可再现。这些是 Luna Innovations 第一阶段研究计划的关键因素，该计划证明了处理组织的创新方法在体外和体内的可行性，以在自体游离脂肪移植 (AFT) 和组织工程过程中实现更有效和可重复的体积和组织活力。第二阶段的目标是完善初步结果并开发临床相关的标准程序，以机械和/或化学方式处理患者的脂肪组织，以在移植前将脂质排出，身体会将组织转化回功能性、健康的脂肪。该技术有望改善最终体积和质地的临床结果，与目前使用的 AFT 实践相比，可预测性和再现性更高。最终，临床系统将由一个全自动和可编程的生物反应器组成，用于执行组织处理，从而产生更有效、更可预测和更可重复的软组织重建，同时消除任何排斥和过敏反应。公共卫生相关性：与颅面手术相关的许多重建挑战的特点是缺乏软组织“填充”和/或对称性。该第二阶段提案的目标是验证一种体外机械脱脂脂肪组织的方法，为自体游离脂肪移植做准备，并设计必要的全自动生物反应器。这种新颖的方法将带来更有效和可重复的增强和增强。一旦商业化，这项技术在重建和美容应用以及伤口愈合方面具有巨大的医疗潜力。