Imaging Core

成像核心

• 批准号: 8119113
• 负责人: ANDREAS H HIELSCHER
• 金额: $ 10.99万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8119113
• 关键词: 3-Dimensional; Animal Model; Animals; Arts; Autopsy; Bioluminescence; Blood; Blood Vessels; Bone Marrow; Development; Devices; Disease; Equation; Fluorescence; Fluorescent Probes; Frequencies; Future; Goals; Hemoglobin; Human; Hypoxia; Image; Imaging Device; Imaging technology; In Situ; Light; Luciferases; Magnetic Resonance; Magnetic Resonance Imaging; Malignant Neoplasms; Methods; Microscope; Microscopic; Microscopy; Modeling; Molecular; Molecular Probes; Myofibroblast; Optical Methods; Optical Tomography; Optics; Process; Research Personnel; Resolution; Science; Staging; Surface; System; Techniques; Technology; Three-Dimensional Image; Tissues; absorption; anticancer research; base; cancer imaging; fluorescence imaging; hemodynamics; image reconstruction; imaging modality; in vivo; migration; non-invasive system; novel; optical imaging; reconstruction; treatment effect; tumor; tumor growth; two-photon

The overall goal of this core is to support the specific aims of the 4 projects with existing small animal imaging technology and to advance this technology to enhance future utility in cancer research. Small animal imaging has gained considerable importance in recent years as more and more animal models for human cancers have become available. Imaging of tumor development and effects of treatments has many scientific and economical advantages, as sacrificing animals at various disease stages and performing necropsy and histopathological studies can be sharply reduced. Optical techniques have proven to be especially valuable when applied to small animal imaging because of an abundance of optical markers (endogenous and exogenous) that can target and visualize various cancer related processes on the cellular and molecular level with comparatively high sensitivities. However, to date most optical imaging studies have only explored whole animal surface imaging without 3-dimensional reconstructions. This limits accurate localization and quantification of observed effects inside the animal. This core focuses on various optical imaging methods that can provide 3-dimensional functional information at high temporal resolution about blood-dependent parameters such as oxy, deoxy, and total hemoglobin, fluorescent markers such as GFP, and bioluminescent probes such as luciferase. Imaging system that will be made available include a two-photon microscope for high-spatial-resolution (<0.1 mu m up to depth of 600 mu m) imaging of hemodynamic effects and fluorescent probes in situ; two dynamic optical tomography devices and one frequency-domain optical tomography system for non-invasive whole-animal absorption imaging; and a Xenogen IVIS 200 system for whole-animal fluorescence and bioluminescence imaging. Together with a 9.4 T magnetic resonance imaging system, which delivers high-resolution anatomical images of small animals, the core will enable researcher to study effects of hypoxia on tumor development, migration of activated myofibroblasts, bone marrow recruitment, and tumor growth and regression. Going beyond applying existing optical technologies, the core will also advance imaging science in itself. First, we will develop novel, highly accurate, three-dimensional image reconstruction capabilities for the existing Xenogen IVIS 200 bioluminescence imager. Second, we will adapt and optimize laminar optical tomography (LOT) for applications in digestive cancer research. LOT promises to be a viable optical imaging modality that can provide absorption and fluorescence imaging of tissues to depths of 2-3mm with 100 to 200 mu m resolution. If successfully applied to cancer imaging, this modality would fill an important niche between the high-resolution two-photon microscope systems and the whole-animal optical imaging devices.

该核心的总体目标是支持现有小动物的4个项目的具体目标 成像技术并推进这项技术，以增强癌症研究的未来效用。小动物 近年来，随着人类的动物模型越来越多，成像变得非常重要 癌症已经可用。肿瘤发育和治疗作用的成像具有许多科学 和经济的优势，作为在各种疾病阶段牺牲动物，进行尸检和 组织病理学研究可以大大减少。事实证明，光学技术特别有价值 当由于大量的光学标记（内源性和 外源性）可以靶向和可视化细胞和分子上各种癌症相关的过程 敏感性相对较高的水平。但是，迄今为止，大多数光学成像研究只探索了 全动物表面成像，没有三维重建。这限制了准确的本地化和 量化动物内部观察到的效果。 该核心专注于可以提供3维功能的各种光学成像方法 有关血液依赖性参数的高时间分辨率的信息，例如氧，脱氧和总数 血红蛋白，荧光标记（例如GFP）和生物发光探针，例如荧光素酶。成像 将提供的系统包括用于高空间分辨率的两光子显微镜（<0.1 mu m up 到600 mu m）血液动力学作用和原位荧光探针的成像；两个动态光学 层析成像设备和一个频域光学断层扫描系统，用于非侵入性全动脉 吸收成像；以及全动物荧光和生物发光的异种IVIS 200系统 成像。连同9.4 t磁共振成像系统一起提供高分辨率解剖学 小动物的图像，核心将使研究人员能够研究缺氧对肿瘤发育的影响， 活化的肌纤维细胞，骨髓募集以及肿瘤生长和回归的迁移。 除了应用现有的光学技术外，核心还将推进成像科学 本身。首先，我们将开发出新颖的，高度准确的三维图像重建功能 现有的Xenogen ivis 200生物发光成像仪。第二，我们将适应和优化层流光学 层析成像（批次）用于消化癌研究中的应用。 Lot有望成为可行的光学成像 可以将组织的吸收和荧光成像的方式与100 to提供2-3mm的深度 200 mu m分辨率。如果成功地应用于癌症成像，这种方式将填充重要的利基市场 在高分辨率的两光子显微镜系统和整个动物光学成像设备之间。