Improved Ratiometric Voltage-Sensitive Dyes for In-Vitro and In-Vivo Imaging of Brain Electrical Signaling

改进的比例电压敏感染料用于脑电信号的体外和体内成像

基本信息

批准号：
10018113
负责人：
Corey Acker
金额：
$ 14.1万
依托单位：
POTENTIOMETRIC PROBES, LLC
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-13 至 2023-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10018113
关键词：
Acute Address Adoption Area BRAIN initiative Back Biological Assay Biomedical Engineering Brain Brain imaging Cardiac Cell Culture Techniques Cells Cellular Assay Chemicals Communication Communities Computer Models Data Development Disease Dyes Family suidae Fluorescent Dyes Fluorine Funding Goals Heart Heart Research Histocompatibility Image Imaging Techniques In Vitro Lead Light Link Market Research Measurable Measurement Microscope Mission Morphologic artifacts Motion Neurons Neurosciences Neurosciences Research Noise Optics Pathologic Performance Phase Photons Preparation Process Reaction Time Reporting Research Research Personnel Sampling Schizophrenia Services Signal Transduction Slice Structure Synapses Technology Testing Time TimeLine Translating autism spectrum disorder brain cell brain tissue commercialization design experience experimental study high throughput screening imaging modality improved in vitro testing in vivo in vivo imaging light intensity live cell imaging nervous system disorder optogenetics ratiometric sensor tool two-photon usability virtual voltage voltage sensitive dye web site

项目摘要

Project Summary/Abstract Potentiometric Probes Voltage-sensitive dyes can be applied to brain cells and tissue to study the electrical signals used by the brain to function. The fluorescent dye molecules light up under a microscope and convert tiny electrical signals to measurable changes in light intensity. Virtually all neurological disorders involve changes in the brain’s normal electrical activity and can be studied with voltage-sensitive dyes. The electrochromic voltage-sensitive dyes developed by the founders of Potentiometric Probes have extremely fast response times, one of several features that make them ideal for studying spiking in the brain’s neuronal networks. However, electrochromic dyes typically suffer from a lack of sensitivity making them difficult to use outside specialized labs. Potentiometric Probes looks to overcome this weakness with new dyes and new imaging techniques that can boost the sensitivity and information conveyed in voltage-imaging experiments. New in-vitro and in-house live cell test assays along with computational modeling will allow better optimization of voltage-sensitivity for recording neuronal spiking with the highest possible signal-to-noise. Fast, electrochromic dyes are compatible with ratiometric imaging, which has important applications for in-vivo recordings. If successful, availability of improved, better validated dyes would make voltage imaging accessible to a much wider body of researchers and provide them with a more powerful tool for studying the electrical signals underlying normal and pathological brain function.

项目摘要/摘要电位探针电压敏感染料可应用于脑细胞和组织以研究大脑使用的电信号荧光染料分子在显微镜下发光并将微小的电信号转换为电子信号。事实上，所有神经系统疾病都涉及大脑正常状态的变化。电活性，可以用电压敏感染料进行研究。由电位探针的创始人开发的具有极快的响应时间，是几种探针之一这些特性使它们成为研究大脑神经网络尖峰的理想选择。染料通常缺乏敏感性，因此很难在专业实验室之外使用。电位探针希望通过新染料和新成像技术来克服这一弱点提高新的体外和内部实时电压成像实验的灵敏度和信息传递。细胞测试分析以及计算模型将允许更好地优化电压灵敏度以尽可能高的信噪比记录神经元尖峰快速、电致变色染料兼容。比率成像，对于体内记录有重要的应用如果成功的话，可以获得。改进的、经过更好验证的染料将使更广泛的研究人员能够使用电压成像并为他们提供更强大的工具来研究正常和不正常的电信号病理性脑功能。