One Way Micropatterns for Self-Assembly and Sorting of Cells

用于细胞自组装和分选的单向微图案

• 批准号: 8013525
• 负责人: CHIA-CHI HO
• 金额: $ 33.65万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-01 至 2014-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8013525
• 关键词: Actins; Address; Adhesions; Adhesives; Area; Automobiles; Biocompatible Materials; Biological; Biomaterials Research; Blood capillaries; Cell Count; Cell Line; Cell Migration Pathway; Cell Separation; Cell Shape; Cell physiology; Cells; Chemicals; Chemotactic Factors; Collaborations; Complex; Cues; Cytoskeleton; Family; Foundations; Funding; GTP-Binding Proteins; Hepatic Stellate Cell; Hepatocyte; Hepatocyte Growth Factor; In Vitro; Island; Lead; Liver; Location; Mammalian Cell; Mechanics; Molecular; Molecular Genetics; Movement; Natural regeneration; Nerve Regeneration; Neurons; Organ; Pattern; Pattern Formation; Positioning Attribute; Process; Public Health; Receptor Protein-Tyrosine Kinases; Regulation; Research; Resolution; Screening procedure; Signal Pathway; Signal Transduction; Structure; Techniques; Technology; Thin Layer Chromatography; Time; Tissue Engineering; Tissues; Vascularization; analog; base; biological research; biological systems; capillary; cell assembly; cell growth; cell motility; cell type; cost; design; directional cell; disease diagnosis; in vivo; migration; multidisciplinary; neuronal cell body; next generation; polymerization; public health relevance; response; rho; scaffold; self assembly; shear stress; trafficking

DESCRIPTION (provided by applicant): Precise control of directional migration and spatial organization of multiple cell types is critical to maintaining and engineering tissue function. A major challenge in biomaterials research is the design and fabrication of biomaterials that can guide the simultaneous self-assembly of large number of cells with the precision and efficiency of biological systems. The proposed research builds on the PI and co-PI's recent discovery of MANDIP - Microarray Amplification of Natural Directional Persistence - to guide the long-range directional migration of attached mammalian cells. Key to MANDIP and the natural directional persistence of freely migrating cells is that the envelope of extended lamellipodia is preferentially aligned to the extended cell body. Through an asymmetric arrangement of cell adhesive microarray islands that acts as a ratchet by restricting lamellipodia attachment, MANDIP imposes directionality with overwhelming compliance, guiding simultaneously the migration of unlimited number of cells independently over arbitrary preset paths of unlimited distance without chemoattractants external fields, or mechanical manipulation. The objective of this proposal is to establish a foundation of MANDIP design strategies whose transformative impact will be demonstrated through the following specific aims: Aim 1: Design one-way micropatterns to guide the directional migration of cells on biomaterials, Aim 2: Direct self-assembly of multiple cell types to mimic liver tissue and promote vascularization, Aim 3: Devise a biological analogue of thin-layer chromatography for sorting cells by their intrinsic motility. Accomplishing these aims will bring to fruition MANDIP's potential in promoting tissue pattern formation and as a ubiquitous low-cost cell-sorting platform analogous to thin layer chromatography. PUBLIC HEALTH RELEVANCE: The application is relevant to public health as a low-cost cell sorting technology for rapid screening/purification of cells or diagnosis of diseases related to cell motility and can be applied to engineer tissues by 3D scaffolds that direct migration of multiple cell types.

描述（由申请人提供）：多种细胞类型的定向迁移和空间组织的精确控制对于维持和工程组织功能至关重要。生物材料研究中的一个主要挑战是生物材料的设计和制造，可以指导大量细胞的同时自组装，并具有生物系统的精确性和效率。拟议的研究建立在PI和Co -Pi最近发现的曼迪普 - 自然方向持久性的微阵列放大 - 指导附着的哺乳动物细胞的远距离定向迁移。 Mandip的关键和自由迁移细胞的自然定向持久性是，延伸层状脂蛋白的包膜优先与扩展的细胞体保持一致。通过限制lamellipodia附件来充当棘轮的细胞粘合剂微阵列岛的不对称布置，Mandip以压倒性的合规性施加了方向性，同时指导无限数量的单元格在无限制的无限型距离的情况下独立地迁移，而无需化学上的外部磁场，而无需外部磁场或机械。 The objective of this proposal is to establish a foundation of MANDIP design strategies whose transformative impact will be demonstrated through the following specific aims: Aim 1: Design one-way micropatterns to guide the directional migration of cells on biomaterials, Aim 2: Direct self-assembly of multiple cell types to mimic liver tissue and promote vascularization, Aim 3: Devise a biological analogue of thin-layer chromatography for sorting cells by their intrinsic motility. 实现这些目标将带来曼迪普在促进组织模式形成的潜力，并成为类似于薄层色谱法的无处不在的低成本细胞分类平台。 公共卫生相关性：该应用与公共卫生相关，作为一种低成本细胞分类技术，用于快速筛查/纯化细胞或与细胞运动相关的疾病的诊断，可以通过直接迁移多种细胞类型的3D脚手架应用于工程师组织。