Stomate-based innate immunity against bacterial infection in Arabidopsis

拟南芥基于气孔的针对细菌感染的先天免疫

• 批准号: 8115516
• 负责人: SHENG YANG HE
• 金额: $ 25.27万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-09 至 2011-08-08
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8115516
• 关键词: Abscisic Acid; Agriculture; Animals; Arabidopsis; Area; Bacteria; Bacterial Infections; Bacterial Toxins; Basic Science; Behavior; Biochemical; Biological Assay; Carbon Dioxide; Cells; Complex; Development; Effectiveness; Escherichia coli O157; Flagellin; Food Contamination; Food Poisoning; Future; Genetic; Goals; Health; Homeostasis; Hormones; Host Defense; Human; Humidity; Immune response; Infection; Knowledge; Left; Light; Lipopolysaccharides; Mediating; Microbe; Microscopy; Molecular; Molecular Genetics; Natural Immunity; Nitric Oxide; Output; Pathogenesis; Pattern; Peptides; Perception; Phosphotransferases; Plant Epidermis; Plant Growth Regulators; Plant Leaves; Plant Model; Plants; Production; Prophylactic treatment; Pseudomonas syringae; Public Health; Research; Role; Salicylic Acids; Signal Transduction; Signal Transduction Pathway; Source; Stomas; Stress; Surface; Testing; Time; Tomatoes; Vegetables; Virulence; Virulence Factors; Virulent; Water; base; cell type; coronatine; improved; movie; novel; pathogen; pathogenic bacteria; plant poison; prevent; public health relevance; receptor; response; ubiquitin-protein ligase; uptake

DESCRIPTION (provided by applicant): Innate immunity in animals and plants can be activated by highly conserved pathogen/microbe- associated molecular patterns (PAMPs/MAMPs), such as bacterial flagellin and lipopolysaccharides (LPSs). Recent studies provide evidence that plant stomata, pores in the epidermis of plants, function in innate immunity against the entry of bacteria into the plant. Specifically, stomata close in response to Escherichia coli O157:H7 and Pseudomonas syringae pv. tomato (Pst) DC3000 or bacterial PAMPs. Perception of PAMPs by guard cells, the pair of cells that forms the stomatal pore, requires a flagellin receptor as well as production and signaling of the plant hormones abscisic acid and salicylic acid. The virulent plant pathogen Pst DC3000 produces the phytotoxin coronatine (COR) to suppress stomatal closure as a virulence strategy. Collectively, these results suggest an important role of stomata in modulating the interactions of plants with plant and human pathogenic bacteria in the phyllosphere (the leaf surface). The long-term goal of this project is to elucidate the signal transduction pathway leading to stomate- based defense and to dissect the mechanism by which the bacterial toxin COR inhibits this novel host defense. The central hypotheses to be tested are that (i) stomate-based defense is an integral part of the plant's natural immune response to restrict the entry of bacteria, including human pathogenic bacteria, and (ii) to be successful, plant pathogens must rely on specific virulence factors or environmental conditions to suppress stomate-based defense. An integrative approach involving molecular genetics, microscopy, and biochemical and pathogenesis assays will be used. The proposed research will contribute to the fundamental knowledge of innate immunity and bacterial pathogenesis in plants and will increase our understanding of the molecular basis of plant-human pathogen interactions in the phyllosphere. Human pathogen contamination of fresh vegetables is a major public health concern and a potential bio-terrorist threat as a source of food poisoning. PUBLIC HEALTH RELEVANCE: This proposal describes basic research on the molecular basis of innate immunity of the model plant Arabidopsis to plant and human pathogenic bacteria. Food contamination with human pathogens is a major concern to public health. This research has the potential to provide a mechanistic understanding of plant defense and human pathogen contamination of fresh produce and to guide future development of appropriate prevention measures.

描述（由申请人提供）：动物和植物的先天免疫力可以通过高度保守的病原体/微生物相关的分子模式（PAMP/MAMP），例如细菌鞭毛蛋白和脂多糖（LPSS）激活。最近的研究提供了证据表明，植物气孔（植物表皮中的毛孔）起作用，可抵抗细菌进入植物。具体而言，气孔响应于大肠杆菌O157：H7和假单胞菌PV。番茄（PST）DC3000或细菌泵。护卫细胞对弹药的感知，这是形成气孔孔的一对细胞，需要鞭毛蛋白受体以及植物激素脱离的植物荷尔蒙和水杨酸的产生和信号传导。有毒的植物病原体PST DC3000产生植物毒素冠状动脉（COR），以抑制气孔闭合作为毒力策略。总的来说，这些结果表明气孔在调节植物与植物和人类致病细菌（叶子表面）的相互作用中的重要作用。 该项目的长期目标是阐明导致基于固定盐的防御的信号转导途径，并剖析细菌毒素COR抑制这种新型宿主防御的机制。要测试的中心假设是（i）基于固定物的防御是植物自然免疫反应的组成部分，以限制包括人类病原细菌在内的细菌的进入，并且（ii）成功，植物病原体必须依靠特定的毒力因子或环境条件来抑制基于stomate的防御。将使用涉及分子遗传学，显微镜以及生化和发病机理测定法的综合方法。拟议的研究将有助于植物中先天免疫和细菌发病机理的基本知识，并将提高我们对植物性植物 - 人类病原体相互作用的分子基础的理解。人类病原体对新鲜蔬菜的污染是一个主要的公共卫生问题，也是潜在的生物恐怖威胁，作为食物中毒的根源。 公共卫生相关性：该提案描述了模型植物拟南芥对植物和人类致病细菌的先天免疫力基础的基础研究。人类病原体的粮食污染是公共卫生的主要关注点。这项研究有可能对新鲜农产品的植物防御和人类病原体污染的机械理解，并指导未来的适当预防措施的发展。