Angle-resolved polarization signal imaging of early receptor potential

早期受体电位的角度分辨偏振信号成像

• 批准号: 8112263
• 负责人: XINCHENG YAO
• 金额: $ 19.84万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8112263
• 关键词: Animals; Area; Axon; Birefringence; Complex; Development; Diagnosis; Early Diagnosis; Electroretinography; Ensure; Evaluation; Foundations; Goals; Image; Investigation; Lead; Light; Lighting; Lobster; Measurement; Methods; Molecular; Nerve; Neurons; Optical Coherence Tomography; Optical Instrument; Optics; Photoreceptors; Physiology; Preparation; Rana; Resolution; Retina; Retinal; Retinal Diseases; Sampling; Signal Transduction; Slice; Solid; Source; Specificity; Speed; Stimulus; Structure; System; Techniques; Technology; Testing; Time; Tissues; Validation; Visible Radiation; direct application; improved; instrument; light intensity; light scattering; optical imaging; receptor; relating to nervous system; response; retinal stimulation; tool

DESCRIPTION (provided by applicant): The objective of this project is to explore the feasibility of polarization signal imaging of early receptor potential (ERP) in intact retinas. Previous investigations have demonstrated the existence of polarization signal response due to ERP-associated birefringence change in isolated photoreceptor outer segments activated by visible light. However, imaging of the ERP-associated birefringence change in intact retina is technically challenging because of complex structure of the retina. In principle, both birefringence and scattering changes can produce transient polarization signals in the retina. In order to achieve robust imaging of ERP-associated birefringence change, it is important to reduce the effect of light scattering on the measurement. In this project, we propose to develop a high speed light-field optical coherence tomography (OCT) imager, and to validate it for optical separation of birefringence- and scattering-associated polarization signals. The proposed light-field OCT imager will provide capability of angle-resolved polarization signal imaging. In principle, when an oblique light illumination is used, synthetic angular filters will allow optical separation of light scattering- and birefringence-associated polarization signals in the light activated retina. Isolated lobster nerve axons will be employed as a transition preparation for functional test of the proposed imager. Frog retinal slices and intact retinas will be used to demonstrate the feasibility of polarization signal imaging of ERP-associated birefringence response. Although our ultimate goal is to use the proposed technology to diagnose of retinal diseases, this R21 project will focus on technical development and functional validation of the light-field imaging system. Successful implementation of this project will build a solid foundation for the development of objective, high resolution instruments for photoreceptor function evaluation. PUBLIC HEALTH RELEVANCE: The proposed light-field imager will provide capability of high resolution imaging of stimulus-evoked early receptor potential. High resolution evaluation of photoreceptor response can lead to better study and diagnosis of the retina.

描述（由申请人提供）：该项目的目的是探索完整视网膜早期受体电位（ERP）极化信号成像的可行性。先前的研究表明，由于可见光激活的分离感光受体外部段中与ERP相关的双重反射变化而导致的极化信号响应。然而，由于视网膜的复杂结构，完整视网膜的ERP相关双折射变化的成像在技术上具有挑战性。原则上，双折射和散射变化都可以在视网膜中产生瞬时极化信号。为了实现与ERP相关的双重变化的可靠成像，降低光散射对测量的影响很重要。在这个项目中，我们建议开发高速光场光学相干断层扫描（OCT）成像仪，并验证其以将双重和散射相关的极化信号的光学分离。所提出的光场OCT成像器将提供角度分辨偏振信号成像的能力。原则上，当使用倾斜的光照射时，合成角过滤器将允许光散射和双向相关的极化信号的光学分离。分离的龙虾神经轴突将被用作提议成像仪功能测试的过渡准备。青蛙视网膜切片和完整的视网膜将用于证明与ERP相关双发反应的极化信号成像的可行性。尽管我们的最终目标是使用拟议的技术诊断视网膜疾病，但该R21项目将着重于技术开发和功能验证光场成像系统。该项目的成功实施将为开发光感受器功能评估的客观高分辨率工具的开发奠定坚实的基础。 公共卫生相关性：拟议的光场成像仪将提供对刺激引起的早期受体潜力的高分辨率成像的能力。光感受器反应的高分辨率评估可以导致视网膜的更好研究和诊断。