Genetic competence apparatus of Bacillus subtilis

枯草芽孢杆菌遗传能力装置

• 批准号: 8031110
• 负责人: DAVID A DUBNAU
• 金额: $ 5.3万
• 依托单位: UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-19 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8031110
• 关键词: Address; Animal Welfare; Bacillus subtilis; Bacteria; Bibliography; Biochemistry; Cell surface; Cells; Collaborations; Competence; Country; DNA; DNA Binding; Environment; Environmental Impact; Equipment; Funding; Genetic; Germany; Hand; IACUC; International; Light; Membrane; Physiological; Pilum; Principal Investigator; Progress Reports; Proteins; Protocols documentation; Published Comment; Reagent; Research; Research Ethics Committees; Resources; Single-Stranded DNA; Study Section; Testing; Text; Update; Vertebrates; abstracting; base; design; expiration; human subject; programs; research study

The long-term objective of the proposed research is to determine the mechanisms of bidirectional transport across the nuclear pore complex. Transport in and out of the nucleus begins with recognition of the transported cargo by a dedicated set of transport receptors. After docking at the nuclear pore, the cargo-receptor complex moves through the pore channel, which is lined with nucleoporins. Loading and unloading of cargoes and interactions of the transported complexes with the nuclear pore are the defining events of nuclear transport. In many cases, the RanGTPase and its regulators controt the direction of movement through the pore. Targeting of large complexes such as the ribosome for export out of the nucleus imposes an even greater complexity on the process of nuclear transport. The Specific Aims are to determine how: 1) ribosomes are exported out of the nucleus; 2) nuclear transport factors make contact with the nuclear pore complex; 3) the organization of the Ran regulators in the nucleus integrates nuclear transport and gene regulation; and 4) the integrity of the nuclear envelope is maintained. Defects in nuclear localization are associated with a number of diseases thus contributing to the health-relatedness of the project. Nuclear-cytoplasmic transport provides a mode of regulation for gene control in response to, for example, growth cues, stress, viral infection, immune response, and activity of tumor suppressors. The activity of certain proteins can be affected by altering their intraceltular location between the cytoplasm and the nucleus making nuclear transport a potential therapeutic target. In sum, understanding the process of nuclear transport and how it is regulated, may lead to new drug targets and ways to enhance viral-based gene therapies.

拟议的研究的长期目标是确定跨核孔复合物的双向转运的机制。进出核的运输始于一组专用的运输受体对运输货物的识别。在核孔对接后，货物受体复合物穿过孔通道，孔通道衬有核孔蛋白。货物的装载和卸载以及运输的复合物与核孔的相互作用是核运输的定义事件。在许多情况下，rangtpase及其调节剂通过孔的运动方向构成了运动的方向。靶向大型复合物（例如从细胞核中出口的核糖体）对核转运过程的复杂性更大。具体目的是确定：1​​）核糖体从核中输出； 2）核转运因子与核孔复合物接触； 3）核中RAN调节剂的组织整合了核转运和基因调节； 4）保持核包膜的完整性。核定位的缺陷与多种疾病有关，从而导致该项目的健康相关性。核质质转运为例如生长提示，应激，病毒感染，免疫反应以及抑制肿瘤抑制因子的活性提供了一种基因控制的调节方式。某些蛋白质的活性可以通过改变细胞质和使核转运为潜在治疗靶靶的细胞核之间的颗粒内位置来影响。总而言之，了解核运输的过程及其如何调节，可能会导致新的药物靶标和增强基于病毒的基因疗法的方法。