ENHANCEMENT OF THE CPRC-SPF RHESUS MONKEY PROGRAM: AIDS THERAPEUTIC AGENT

加强 CPRC-SPF 恒河猴计划：艾滋病治疗剂

• 批准号: 8359537
• 负责人: Edmundo Nelson Kraiselburd
• 金额: $ 40.35万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8359537
• 关键词: Acquired Immunodeficiency Syndrome; Adolescent; Adult; Animals; Biomedical Research; Breeding; Complex; Female; Future; Infant; Island; Macaca; Macaca mulatta; Maintenance; Monkeys; Production; Research; Retroviridae; SIV; Surveys; Therapeutic Agents; United States National Institutes of Health; Virus; cohort; immature animal; meetings; offspring; programs; social group

DESCRIPTION (Provided by applicant): The objective of this proposal is to enhance the existing SPF rhesus macaque supply and breeding colony program at the SSFS of the CPRC. This program will use genetically characterized, major histocompatability complex (MHC)-typed, Indian-origin monkeys from established social groups obtained from the CPRC's free-ranging colony on the island of CS. The CPRC will donate the founding stock for the colony, which consists of 102 adult and 21 juvenile females and minimum of 20 sires. Previous surveys have shown that the Cayo Santiago macaques are free of several important viruses including retroviruses, and that the majority of immature animals are negative for B virus. About 20-25% of these monkeys are also Mamu-A*01 positive. Currently, there is a shortage of SPF rhesus monkeys for biomedical research and the demand for these animals is expected to increase dramatically in the future. The establishment and maintenance of this expanded program at SSFS will help meet the increased demand for both SPF (B virus, SRV-D, SIV, and STLV-l-free) and Mamu-A*01 positive rhesus monkeys. MHC-typing and selective breeding will be used to increase the production of SPF Mamu-A*01 positive offspring. The founding cohort is expected to produce a minimum of 70 infants during the first year and an estimated total offspring of about 400 SPF animals in five years. By Year 6 the program should be self-supporting. The CPRC's program will make a significant contribution to advancing biomedical research, and in particular to AIDS research by providing high quality and healthy SPF rhesus monkeys to NIH-sponsored research programs.

描述（由申请人提供）：本提案的目的是加强 CPRC SSFS 现有的 SPF 恒河猴供应和繁殖群计划。 该项目将使用具有遗传特征的主要组织相容性复合体 (MHC) 型、印度起源的猴子，这些猴子来自已建立的社会群体，这些猴子是从 CPRC 在 CS 岛上的自由放养的群体中获得的。 CPRC 将捐赠该群体的创始种群，其中包括 102 只成年雌性和 21 只幼年雌性以及至少 20 头公牛。 此前的调查显示，圣地亚哥岛猕猴体内没有包括逆转录病毒在内的多种重要病毒，并且大多数未成熟动物的 B 病毒呈阴性。 这些猴子中大约 20-25% 也是 Mamu-A*01 阳性。 目前，用于生物医学研究的SPF级恒河猴短缺，预计未来对这些动物的需求将急剧增加。 在 SSFS 建立和维护这一扩展计划将有助于满足对 SPF（不含 B 病毒、SRV-D、SIV 和 STLV-1）和 Mamu-A*01 阳性恒河猴不断增长的需求。 MHC 分型和选择性育种将用于增加 SPF Mamu-A*01 阳性后代的产量。 创始群体预计在第一年至少产下 70 只幼崽，并预计在五年内产生约 400 只 SPF 动物的后代。到第六年，该计划应该是自给自足的。 CPRC 的项目将为 NIH 资助的研究项目提供高质量和健康的 SPF 恒河猴，为推进生物医学研究，特别是艾滋病研究做出重大贡献。