STRUCTURE OF THE CYTOCHROME C PEROXIDASE INTERMEDIATE COMPOUND I

细胞色素C过氧化物酶中间体化合物I的结构

• 批准号: 8169922
• 负责人: S M SOLTIS
• 金额: $ 0.85万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169922
• 关键词: 2-cyclopentyl-5-(5-isoquinolylsulfonyl)-6-nitro-1H-benzo(D)imidazole; Complex; Computer Retrieval of Information on Scientific Projects Database; Cytochrome c Peroxidase; Data; Data Set; Enzymes; Freezing; Funding; Grant; Heme; Institution; Iron; Methods; Nitrogen; Reaction; Research; Research Personnel; Resolution; Resources; Robot; Series; Source; Spectrum Analysis; Structure; Temperature; United States National Institutes of Health

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cytochrome c peroxidase (CCP) is an intercellular heme enzyme that oxidizes substrates in a multi-step reaction. The iron atom in Compound I is purportedly oxidized from +3 to +4 forming an oxyferryl center in the first step. Attempts to collect x-ray diffraction data from the intermediate compound I have been complicated due to auto-reduction of the complex from the incident x-rays We have been studying the auto-reduction rates using VIS spectroscopy. We have determined that at 67 K, ~4 exposures can be collected from moderately sized crystals to 1.3 A resolution. The temperature is just above the freezing point of nitrogen, while preserving 85% of Compound 1. Methods are being developed to use the robot to screen and collect data from a series of crystals to build a complete data set.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 细胞色素 c 过氧化物酶 (CCP) 是一种细胞间血红素酶，可通过多步反应氧化底物。 据称，化合物 I 中的铁原子在第一步中从 +3 氧化为 +4，形成氧铁基中心。 由于复合物从入射 X 射线中自动还原，从中间体化合物 I 收集 X 射线衍射数据的尝试变得很复杂。我们一直在使用 VIS 光谱研究自动还原率。 我们已经确定，在 67 K 下，可以从中等大小的晶体收集约 4 次曝光，分辨率达到 1.3 A。 温度略高于氮气的冰点，同时保留了 85% 的化合物 1。正在开发使用机器人筛选和收集一系列晶体数据以构建完整数据集的方法。