Developing transposon methods for insertional mutagenesis in Xenopus

开发非洲爪蟾插入突变的转座子方法

• 批准号: 7895047
• 负责人: Mustafa K Khokha
• 金额: $ 20.69万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-16 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7895047
• 关键词: Biological; Biological Models; Biology; Data; Development; Developmental Biology; Embryonic Pattern Specification; Genes; Genetic; Genetic Screening; Genome; Genotype; Goals; Human Genome; Insertional Mutagenesis; Laboratories; Methods; Modeling; Mus; Mutagenesis; Mutagens; Mutate; Pattern Formation; Phenotype; Process; Rana; Research Personnel; Series; Site; System; Xenopus; analytical tool; gene function; genetic analysis; human disease; interest; new technology; novel; public health relevance; research study; tool; tool development; xenopus genome

DESCRIPTION (provided by applicant): The main goal of this proposal is to develop transposon tools in order to mutagenize the genome of Xenopus tropicalis. Recently, we have developed genetic analysis in Xenopus which is an exciting new avenue for studying gene function in this model system that is uniquely manipulable as an experimental system. A critical next step is to develop efficient mutagenesis methods. We outline a series of experiments to develop tol2 and piggybac transposons as insertional mutagens in X. tropicalis. These experiments will demonstrate that insertional mutagenesis with transposons can be an effective way to move quickly from interesting phenotype to genotype facilitating in-depth characterization of gene function. Our major interest is in early embryonic pattern formation; however, the tools that we develop in this proposal will have a wide application in diverse fields of biology. PUBLIC HEALTH RELEVANCE: The function of most genes in our genome remains a mystery. In order to determine their function, we must use model systems such as frogs specifically Xenopus. In this proposal, we will create new technologies to determine gene function in Xenopus and therefore human disease.

描述（由申请人提供）：该提案的主要目标是开发转座子工具，以使Xenopus tropicalis的基因组诱变。最近，我们在Xenopus中开发了遗传分析，这是一种令人兴奋的新途径，用于研究该模型系统中的基因功能，它可以独特地操纵作为实验系统。下一步的关键是开发有效的诱变方法。我们概述了一系列实验，以开发Tol2和Piggybac转座子作为X. Tropicalis中的插入诱变剂。这些实验将表明，用转座子插入诱变可能是一种从有趣的表型转变为促进基因功能深入表征的基因型的有效方法。我们的主要兴趣是早期胚胎模式形成。但是，我们在该提案中开发的工具将在生物学领域中广泛应用。公共卫生相关性：我们基因组中大多数基因的功能仍然是一个谜。为了确定其功能，我们必须使用模型系统，例如青蛙特别是Xenopus。在此提案中，我们将创建新技术来确定爪蟾和人类疾病中的基因功能。

项目成果

Breeding based remobilization of Tol2 transposon in Xenopus tropicalis.

• DOI: 10.1371/journal.pone.0076807
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Lane MA;Kimber M;Khokha MK
• 通讯作者: Khokha MK