Systems Biology Core

系统生物学核心

基本信息

批准号：
10016361
负责人：
ROBERT POWERS
金额：
$ 39.52万
依托单位：
UNIVERSITY OF NEBRASKA LINCOLN
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-15 至 2021-09-13
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10016361
关键词：
Address Area Biochemical Bioinformatics Cellular biology Chemicals Collection Communication Communication Research Communities Computer Models Core Facility Data Data Analyses Data Collection Ensure Experimental Designs Faculty Fee-for-Service Plans Funding Goals Grant Human Resources Individual Industrialization Infrastructure Logistics Mass Spectrum Analysis Methodology Microscopy Modeling NMR Spectroscopy Nebraska Nuclear Magnetic Resonance Pathway interactions Preparation Proteomics Protocols documentation Research Research Design Research Personnel Research Support Sampling Services Specialist Statistical Data Interpretation Structure Support System Systems Biology Technical Expertise Techniques Technology Time Training United States National Institutes of Health Universities base complex biological systems data acquisition data archive data management data mining design field study instrumentation member metabolomics organizational structure programs research facility skills statistics tool

项目摘要

ABSTRACT Researchers in the Nebraska Center for Integrated Biomolecular Communication (CIBC) will be investigating complex biological systems. As a result, these researchers expect to make extensive use of mass spectroscopy (MS), microscopy, and nuclear magnetic resonance (NMR) spectroscopy. These capabilities in systems biology are currently available through three existing University of Nebraska-Lincoln (UNL) core facilities. However, these separate, distinct cores present practical and logistic impediments to researchers needing to effectively incorporate systems biology into their projects. The existing core structure requires an investigator to duplicate effort by interacting with each separate core and devising repetitive protocols. In addition, each individual core focuses on applying its specific expertise and technology to a problem instead of a broad, synergistic view of all alternatives. A systems biology core facility that incorporates all systems biology instrumentation, methodologies, and expertise under a single organizational structure will help ensure that UNL biomedical investigators receive support and training in the systems biology techniques that best fit their research needs. Thus, the establishment of the Systems Biology Core (SBC) facility will address the specific, critical requirements of CIBC investigators. The SBC facility's long-term goal is to create a sustainable systems biology facility that meets the omics and microscopy needs of CIBC members, UNL faculty, and the larger scientific community. The SBC facility's short-term goal is to facilitate the adaption of systems biology technologies by CIBC and UNL investigators. The SBC will accomplish its goals by training and educating investigators in systems biology methodology and by continually expanding and enhancing SBC capabilities, instrumentation, technologies, and capacity. Through the SBC facility, CIBC members will have routine interactions with skilled omics and microscopy experts who can assist with study design, sample preparation, and the collection, analysis, interpretation, and modeling of resulting data. SBC personnel will also collaborate with the Center's Data Management and Analysis Core to facilitate CIBC projects that require data mining and archiving, statistical analysis, functional and predictive analyses, and computational modeling. Thus, the SBC facility will enhance infrastructure in an area of critical importance to CIBC investigators and to UNL faculty more broadly. Establishing the SBC core will require accomplishing three specific aims: 1) Engage a director who will oversee the core; 2) Hire two new staff experts in NMR and MS metabolomics to advise investigators on study design, sample preparation, and data acquisition, analysis, and interpretation; and 3) Efficiently provide omics and microscopy assistance to CIBC projects to ensure investigators have adequate, timely access to core services. As a centralized facility, the SBC will enable Center researchers to effectively integrate systems biology methodologies into their projects, thus enhancing existing infrastructure and providing streamlined access to systems biology instrumentation and methodology.

抽象的内布拉斯加州综合生物分子通讯中心（CIBC）的研究人员将进行调查复杂的生物系统。因此，这些研究人员希望广泛利用质量光谱 (MS)、显微镜和核磁共振 (NMR) 光谱。这些能力在系统生物学目前可通过内布拉斯加大学林肯分校 (UNL) 的三个现有核心课程获得设施。然而，这些独立的、不同的核心给研究人员带来了实际和逻辑上的障碍需要有效地将系统生物学纳入他们的项目中。现有的核心结构需要一个研究人员通过与每个单独的核心交互并设计重复的协议来重复工作。在此外，每个核心都专注于将其特定的专业知识和技术应用于解决问题，而不是对所有替代方案的广泛、协同的看法。包含所有系统生物学的系统生物学核心设施单一组织结构下的仪器、方法和专业知识将有助于确保 UNL 生物医学研究人员获得最适合他们的系统生物学技术的支持和培训研究需要。因此，系统生物学核心（SBC）设施的建立将解决特定的、 CIBC 调查员的关键要求。 SBC 设施的长期目标是创建一个可持续的系统满足 CIBC 成员、UNL 教职人员和更大机构的组学和显微镜需求的生物学设施科学界。 SBC 设施的短期目标是促进系统生物学的适应 CIBC 和 UNL 调查人员的技术。 SBC 将通过培训和教育来实现其目标系统生物学方法论的研究人员，并通过不断扩展和增强 SBC 能力，仪器、技术和能力。通过 SBC 设施，CIBC 会员将有例行的与熟练的组学和显微镜专家互动，他们可以协助研究设计、样品制备、以及结果数据的收集、分析、解释和建模。 SBC人员也将合作与中心的数据管理和分析核心一起促进需要数据挖掘和分析的 CIBC 项目归档、统计分析、功能和预测分析以及计算建模。因此，SBC 该设施将加强对 CIBC 调查人员和 UNL 教师至关重要的领域的基础设施更广泛地说。建立 SBC 核心需要实现三个具体目标：1）聘请董事谁将监督核心； 2) 聘请两名新的 NMR 和 MS 代谢组学专家为研究人员提供建议研究设计、样品制备以及数据采集、分析和解释； 3) 高效为 CIBC 项目提供组学和显微镜援助，以确保研究人员获得充分、及时的信息访问核心服务。作为一个集中设施，SBC 将使中心研究人员能够有效地将系统生物学方法整合到他们的项目中，从而增强现有的基础设施和提供对系统生物学仪器和方法的简化访问。