Mechanisms for Wound Healing with Ultrasound

超声波伤口愈合机制

基本信息

批准号：
7918844
负责人：
DIANE DALECKI
金额：
$ 49.88万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-30 至 2013-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7918844
关键词：
Acoustics Active Sites Adverse effects Affect Amputation Asthma Binding Sites Biological Biology Blood Vessels Blood flow Burn injury Cell Adhesion Cell Migration Pathway Cell Surface Receptors Cell physiology Cells Chronic Chronic Disease Collagen Complex Data Decubitus ulcer Deposition Development Diabetes Mellitus Elderly Elements Engineering Epithelial Cells Extracellular Matrix Fibroblasts Fibronectins Gel Goals Growth Healed Healthcare Heparin Binding Impaired wound healing Infection Infection prevention Injury Kidney Diseases Knowledge Lead Leg Ligation Liquid substance Liver Cirrhosis Mechanics Mediating Methods Molecular Conformation Muscle Contraction Myofibroblast Normal tissue morphology Pathway interactions Patients Perfusion Physiologic pulse Process Quality of life Role Scientist Signal Transduction Site Skeletal Muscle Structure System Technology Tensile Strength Therapeutic Effect Therapeutic Uses Tissue Model Tissues Ultrasonic Therapy Ultrasonography Vasodilation Wound Healing bone healing care burden cell behavior cell growth cell motility chemotherapy design diabetic economic cost healing health care economics improved in vivo injured mouse model multidisciplinary novel polymerization public health relevance response to injury soft tissue tissue regeneration ultrasound biological effect wound

项目摘要

DESCRIPTION (provided by applicant): There is a critical need for new disruptive technologies to accelerate or improve the healing of chronic soft tissue wounds. Chronic wounds, including diabetic, leg, and pressure ulcers, impose a significant health care burden worldwide. Currently, chronic wound therapy is primarily supportive. Novel treatments that effectively stimulate specific wound healing pathways would greatly reduce healthcare and economic costs, lessen the chance of amputation, and improve the quality of life of patients with chronic wounds. The extracellular matrix (ECM) provides a complex array of cell adhesion sites, cell migration pathways, and proliferation signals to cells, and imparts mechanical stability to the healing wound. Mechanical forces influence the deposition, organization, and structure of ECM fibronectin fibrils, which in turn, affects cell function, ECM organization and stability, tissue tensile strength, and vascular perfusion. Ultrasound (US) is currently used clinically to promote bone healing and has been shown to enhance soft tissue repair. Certain biological effects of US are known to occur through non-thermal, mechanical mechanisms. Thus, we hypothesize that mechanical forces associated with US propagation are capable of remodeling fibronectin in chronic wounds to expose biologically active sites that, in turn, enhance myofibroblast growth and contractility, stimulate epithelial cell migration, promote collagen organization and mechanical strength, and increase blood flow to tissues. In this proposal, we have assembled a multidisciplinary team of scientists, engineers, and clinicians with expertise in cell and ECM biology, biomedical ultrasound and acoustics, vascular biology, and wound healing. We will use noninvasive US fields to identify key biological and physical mechanisms for US-enhanced soft tissue wound healing in order to develop the use of US for chronic wound therapy. Knowledge of basic mechanisms provides the power to design optimized exposure parameters, identify synergistic therapies, and engineer exposure systems that maximize the therapeutic effects of US while minimizing adverse side effects. Public Health Relevance Statement (provided by applicant): Chronic wounds, including diabetic, leg, and pressure ulcers, impose a significant health care burden worldwide. New treatment methods are needed to rapidly close burns and chronic wounds, prevent infection and fluid loss, and promote the natural healing process. In this proposal, we develop the use of noninvasive ultrasound fields to accelerate tissue repair.

描述（由申请人提供）：新的破坏性技术迫切需要加速或改善慢性软组织伤口的愈合。包括糖尿病，腿部和压力溃疡在内的慢性伤口在全球范围内造成了重大的医疗保健负担。目前，慢性伤口疗法主要支持。有效刺激特定伤口愈合途径的新型治疗方法将大大降低医疗保健和经济成本，减少截肢的机会，并改善慢性伤口患者的生活质量。细胞外基质（ECM）提供了一个复杂的细胞粘附部位，细胞迁移途径和增殖信号，并向细胞赋予机械稳定性，以赋予愈合伤口。机械力影响ECM纤连蛋白原纤维的沉积，组织和结构，这反过来影响细胞功能，ECM组织和稳定性，组织拉伸强度和血管灌注。超声（US）目前用于临床促进骨骼愈合，并已显示可增强软组织修复。已知我们的某些生物学作用是通过非热机械机制发生的。因此，我们假设与美国传播相关的机械力能够重塑慢性伤口中的纤连蛋白，以暴露生物学活性部位，从而增强肌纤维细胞的生长和收缩力，刺激上皮细胞迁移，促进胶原蛋白组织和机械强度，并增加血液流向组织。在这项建议中，我们组建了一个由科学家，工程师和临床医生组成的多学科团队，具有细胞和ECM生物学，生物医学超声和声学，血管生物学以及伤口愈合方面的专业知识。我们将使用无创的美国领域来识别美国增强软组织伤口愈合的关键生物学和物理机制，以开发使用我们用于慢性伤口疗法的使用。基本机制的知识为设计优化的暴露参数，确定协同疗法以及工程师暴露系统提供了能力，从而最大程度地提高了我们的治疗效果，同时最大程度地减少了不良副作用。公共卫生相关性声明（由申请人提供）：包括糖尿病，腿部和压力溃疡在内的慢性伤口在全球造成了巨大的医疗保健负担。需要新的治疗方法来快速闭合烧伤和慢性伤口，防止感染和液体丧失，并促进自然愈合过程。在此提案中，我们开发了非侵入性超声场的使用来加速组织修复。