Facility Core E: Cell & Tissue Analysis

设施核心 E：单元

• 批准号: 8055910
• 负责人: Ellen R Richie
• 金额: $ 9.59万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8055910
• 关键词: Apoptotic; Cell Cycle; Cell Separation; Cells; Complement; Confocal Microscopy; Consultations; Core Facility; Cytoplasmic Protein; Flow Cytometry; Organelles; Proteins; Research Personnel; Resolution; Services; Subcellular structure; Surface; Suspension substance; Suspensions; Tissues; Training; base; cell type; digital imaging; protein expression; research study; single cell analysis

A. Overall Objectives And Specific Aims The Cell and Tissue Analysis Facility Core includes flow cytometry and confocal microscopy services. The flow cytometry service provides Center investigators with the ability to quantify surface and/or cytoplasmic protein expression in a variety of cell types, analyze the fraction of apoptotic cells, evaluate cell cycle parameters and sort cells into purified subsets based on these and other criteria. Confocal microscopy complements the quantitative advantages of flow cytometry by localizing cells, organelles or other subcellular components that express particular surface or cytoplasmic proteins in tissue sections using high-resolution digital imaging. The Specific Aims of the Cell and Tissue Analysis Facility Core are: 1. To utilize flow cytometry for multiparameter analysis of single cell suspensions and for isolation of purified subsets by cell sorting 2. To utilize confocal microscopy for precise localization of proteins and subcellular structures. 3. To provide training and consultation on planning, executing and evaluating experiments involving flow cytometry and confocal microscopy

A.总体目标和特定目标 细胞和组织分析设施核心包括流式细胞仪和共聚焦显微镜服务。这 流式细胞仪服务为中心研究人员提供了量化表面和/或细胞质的能力 多种细胞类型中的蛋白质表达，分析凋亡细胞的分数，评估细胞周期 基于这些和其他标准，参数并将细胞分为纯化的子集。共聚焦显微镜 通过定位细胞，细胞器或其他亚细胞来补充流式细胞仪的定量优势 使用高分辨率在组织切片中表达特定表面或细胞质蛋白的成分 数字成像。 细胞和组织分析设施核心的具体目的是： 1。利用流式细胞仪进行单细胞悬浮液的多参数分析和分离 通过细胞分类纯化子集 2。利用共聚焦显微镜进行蛋白质和亚细胞结构的精确定位。 3。提供有关涉及计划，执行和评估实验的培训和咨询 流式细胞仪和共聚焦显微镜