Mechanisms of Olfactory Transduction

嗅觉传导机制

• 批准号: 7850378
• 负责人: Barry W. ACHE
• 金额: $ 37.29万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-17 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7850378
• 关键词: Aging; Animal Model; Biochemical; Cations; Chemicals; Cloning; Competence; Complex; Cyclic Nucleotides; Disease; Event; Functional disorder; GTP-Binding Proteins; Inositol Phosphates; Invertebrates; Ion Channel; Lobster; Mediating; Molecular; Neurons; Nose; Odors; Olfactory Receptor Cells; Olfactory Receptor Neurons; Output; Pharmaceutical Preparations; Phosphatidylinositols; Phospholipids; Phosphorylation; Play; Quality of life; Receptor Cell; Regulation; Relative (related person); Role; Signal Pathway; Signal Transduction; Smell Perception; System; Taste Perception; in vivo; receptor

The sense of smell plays an increasingly appreciated role in our general quality of life and well being, a role that is compromised by the effect of disease, drugs, aging and environmental onslaught on our chemosensory competence. Receptor neurons in the nose serve the critical function of detecting and transducing odorants. Disruption of any of the intracellular events leading to receptor cell activation can result in olfactory dysfunction. Emerging evidence implicates phosphoinositide signaling and the transient receptor potential (TRP) ion channels commonly targeted by this signaling pathway in chemosensory transduction in taste receptor cells, vomeronasal receptor neurons, invertebrate olfactory receptor neurons, and possibly in vertebrate olfactory receptor neurons, where recent evidence suggests it can modulate the well established cyclic nucleotide signaling pathway. Phosphoinositide signaling is inherently complex, and this complexity has impeded a clear understanding of its role in many cellular systems. In this continuing project, we use an integrated, molecular, biochemical, and electrophysiologicalapproach to further define the cellular mechanisms of phosphoinositide signaling in an olfactory system. We use an animal model in which the functional role of phosphoinositide signaling in olfactory transduction is particularly well established, and has led to the evidence suggesting a potential role for phosphoinositide signaling in vertebrate olfaction. This project has the potential to identify new cellular and molecular events potentially involved in olfactory receptor cell activation, as well as contribute to a more general understanding of phospholipid signaling and TRP channel function in chemical sensing.

嗅觉在我们的一般生活质量和福祉中起着越来越欣赏的作用 疾病，药物，衰老和环境对我们的影响所损害的角色 化学感应能力。鼻子中的受体神经元具有检测和 转导的气味。破坏导致受体细胞激活的任何细胞内事件可以 导致嗅觉功能障碍。新兴证据暗示磷酸肌醇信号和瞬态 该信号通路在化学感觉中通常针对的受体电位（TRP）离子通道 味觉受体细胞，呕吐受体神经元，无脊椎动物嗅觉受体神经元的转导， 可能在脊椎动物嗅觉受体神经元中，最近的证据表明它可以调节 良好的环状核苷酸信号通路。磷酸肌醇信号本质上是复杂的，并且 这种复杂性阻碍了对其在许多细胞系统中的作用的清晰了解。在这个继续 项目，我们使用综合，分子，生化和电生理方法进一步定义 嗅觉系统中磷酸肌醇信号传导的细胞机制。我们使用动物模型 磷酸肌醇信号在嗅觉转导中的功能作用特别好 已建立，并提出了证据，表明磷酸肌醇信号在 脊椎动物嗅觉。该项目有可能识别新的细胞和分子事件 参与嗅觉受体细胞激活，并有助于对 化学传感中的磷脂信号传导和TRP通道功能。