Wichita State University Combined Core Facility Renovation project

威奇托州立大学联合核心设施改造项目

• 批准号: 7935973
• 负责人: WILLIAM J HENDRY
• 金额: $ 219.1万
• 依托单位: WICHITA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-18 至 2013-03-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7935973
• 关键词: 20 year old; Aging; Applications Grants; Area; Award; Biochemical; Biological Sciences; Biology; Cell Culture Techniques; Core Facility; Coupled; Development; Discipline; Educational process of instructing; Emergency Situation; Ensure; Equipment; Faculty; Floor; Foundations; Goals; Grant; Housing; Human Follicle Stimulating Hormone; Individual; Internet; Laboratories; Molecular; Molecular Biology; Physiological; Program Research Project Grants; Property; Protein Chemistry; Proteins; Research; Research Activity; Research Personnel; Research Support; Scientist; United States National Institutes of Health; Universities; Wichita; Wireless Technology; Work; age related; base; design; facility renovation; instrument; interest; meetings; member; organizational structure; pituitary gonadal axis; programs; protein expression; protein purification; reproductive; success

DESCRIPTION (provided by applicant): The WSU Combined Core Facility Renovation Project proposal described herein outlines plans to upgrade significantly the Wichita State University (WSU) Department of Biology Core Lab Facility. The Labs comprising the Core were established as separate core laboratories 20 years ago by a Wesley Foundation grant. They have recently been reinvigorated and reorganized through the awarding of a 5-year NIH P01 Program Project entitled "The Aging Pituitary/Gonadal Axis" (1P01AG029531). This multi-investigator, multi-institutional program project is centered at WSU with Dr. George Bousfield as its PI and is focused upon the age-related changes in the expression of human FSH glycoforms and the differential biochemical and physiological properties manifest by these changes. Within the P01, three Core Lab functions are consolidated within overall Core B. These 3 functions include protein expression, protein purification and characterization, and protein functional analysis, which involve three fundamental disciplines, molecular biology, protein chemistry, and cell culture. These labs thus form the basis of the Molecular, Protein, and Cell Culture Cores. In addition, a departmental "common equipment room" housing many instruments utilized in the P01 Project has been re-designated as the Equipment Core. These four Core Labs have been organized functionally and administratively into the Combined Core Facility. This Facility now has defined organizational structure with an overall Core Facility Leader and Individual Core Lab Leaders supported in part by the P01 grant. The P01 grant represents the initial step towards the long-term goal of WSU scientists to establish a Center for Reproductive Aging (WSU CFRA). Central to efficient and successful progress of the P01 project and development of the Center is the renovation of the Core Facility and the upgrading of Core Facility equipment. The Core Facility is housed in the Department of Biological Sciences on the 4th floor of Hubbard Hall. Built in 1973, Hubbard Hall has seen neither major additions nor major renovations to the labs. Moreover, the labs were designed to meet the needs of the undergraduate teaching not research. The Combined Core Facility currently has numerous deficiencies that impact the ability to carry out efficient work and impair the ability of the Facility to support proposed increases in research activity. Deficiencies include: the poor condition of laboratory benches, cabinets, and sinks, lack of wireless Internet access, inefficient placement of power plugs, lack of emergency power for key pieces of equipment, inefficient lab space configuration, lack of eyewash/shower stations, inappropriate fume hoods, and inconsistent ADA compliance. Additional deficiencies reflect key Core Facility Equipment, which is uniformly 10-20 years old. The goal of this project is to remedy the above deficiencies through renovation of 23 areas comprising the 4 Labs comprising the Combined Core Facility and re-equipping the Labs. The proposed Core Facility renovations coupled with the procurement of Core Facility equipment will provide immense benefit that will: (a) ensure the success of ongoing projects defined within the P01 Program; (b) provide evidence of scientific commitment for anticipated renewal of the P01 in 2014; (c) provide a basis for expanded research efforts in terms of a new grant applications that directly relate to the P01; (d) provide continued and expanded support for current laboratory-based scientists both within the department and those affiliated with the department; and (e) provide support for the recruitment of new faculty into the department, some of whom will have research interests consistent with the focus of the P01 Program. The P01 Grant represents the largest investigator-initiated grant ever awarded to a WSU faculty member. This renovation proposal is intended to ensure completion of the P01 goals and allow expansion to facilitate support of research within the Department of Biological Sciences.

描述（由申请人提供）：本文所述的 WSU 联合核心设施改造项目提案概述了大幅升级威奇托州立大学 (WSU) 生物系核心实验室设施的计划。组成核心的实验室是 20 年前由韦斯利基金会资助建立的独立核心实验室。最近，通过授予为期 5 年的 NIH P01 计划项目，题为“衰老的垂体/性腺轴”(1P01AG029531)，他们重新焕发了活力并进行了重组。这个多研究者、多机构的项目以华盛顿州立大学为中心，由 George Bousfield 博士担任 PI，重点研究人类 FSH 糖型表达的年龄相关变化以及这些变化所表现出的差异生化和生理特性。在P01中，三个核心实验室功能被整合到整个核心B中。这三个功能包括蛋白质表达、蛋白质纯化和表征以及蛋白质功能分析，其中涉及三个基础学科：分子生物学、蛋白质化学和细胞培养。因此，这些实验室构成了分子、蛋白质和细胞培养核心的基础。此外，部门的“公用设备室”内存放着P01项目中使用的许多仪器，已被重新指定为设备核心。这四个核心实验室在功能和管理上已被组织到联合核心设施中。该设施现在已经确定了组织结构，其中包括总体核心设施领导者和个人核心实验室领导者，部分由 P01 拨款支持。 P01 拨款代表了 WSU 科学家朝着建立生殖老龄化中心 (WSU CFRA) 的长期目标迈出的第一步。 P01项目高效、成功推进和中心发展的核心是核心设施的改造和核心设施设备的升级。核心设施位于哈伯德大厅四楼的生物科学系。哈伯德大厅建于 1973 年，实验室既没有进行过重大扩建，也没有进行过重大改造。此外，实验室的设计是为了满足本科生教学的需要，而不是研究的需要。联合核心设施目前存在许多缺陷，影响了开展高效工作的能力，并削弱了该设施支持拟议增加研究活动的能力。缺陷包括：实验台、柜子和水槽状况较差，缺乏无线互联网接入，电源插头放置效率低下，关键设备缺乏应急电源，实验室空间配置效率低下，缺乏洗眼/淋浴站，不适当的设施通风柜，以及不一致的 ADA 合规性。其他缺陷还体现在关键核心设施设备上，这些设备的使用年限均是 10-20 年。该项目的目标是通过对包括联合核心设施的 4 个实验室在内的 23 个区域进行翻修并重新装备实验室来弥补上述缺陷。拟议的核心设施改造加上核心设施设备的采购将带来巨大的好处，这将： (a) 确保 P01 计划中定义的正在进行的项目取得成功； (b) 提供预计于 2014 年更新 P01 的科学承诺证据； (c) 为扩大与 P01 直接相关的新赠款申请方面的研究工作提供基础； (d) 为该部门及其附属部门现有实验室科学家提供持续和扩大的支持； (e) 为该系招聘新教员提供支持，其中一些教员的研究兴趣与 P01 计划的重点一致。 P01 补助金是有史以来授予 WSU 教职人员的最大一笔由研究者发起的补助金。该改造提案旨在确保完成 P01 目标，并允许扩展以促进对生物科学系内研究的支持。