Animal Exercise and Analysis Core

动物运动和分析核心

基本信息

批准号：
9789125
负责人：
MARK Louis JOHNSON
金额：
$ 30.05万
依托单位：
INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9789125
关键词：
Aging Aminoisobutyric Acids Animals Architecture Biological Process Biomechanics Bone Density Calibration Cost Savings Data Data Analyses Dinoprostone Endpoint Determination Ensure Estrogen Receptor alpha Estrogen Receptor beta Estrogen Receptors Exercise Femur Goals Guidelines Harvest Image Knock-out Knockout Mice Mediating Methodology Methods Molecular Monitor Mus Muscle Muscle function Musculoskeletal System Osteocytes Postdoctoral Fellow Process Program Research Project Grants Property Protocols documentation Quality Control Regulation Reproducibility Research Research Design Role Running Sampling Serum Services Signal Pathway Signal Transduction Site Skeletal Muscle Soleus Muscle Standardization Technology Testing Tissues Transgenic Organisms age effect age-related muscle loss base beta catenin bone bone mass cortical bone data acquisition experimental study extensor digitorum extracellular vesicles metabolomics microCT mouse model muscle form performance site programs student training substantia spongiosa success

项目摘要

ABSTRACT Core C, the Animal and Analysis Core, will support various aspects of all subprojects within the Program Project Grant and is vital to the success of each. The overall aim of the program project is to understand the mechanisms underlying crosstalk between muscle and bone that may contribute to the age related decline in muscle and bone mass and function. The proposed projects will also investigate whether muscle-bone crosstalk mediates some of the beneficial effects of exercise on the musculoskeletal system and if exercise (voluntary wheel running; VWR) can maintain bone-muscle crosstalk during aging. The Specific Aims of this Core are: Aim 1: Perform VWR experiments, soleus (SOL) and extensor digitorum longus (EDL) muscle contractility on VWR and control mice for Projects 1-4 and provide centralized processing and distribution of samples (conditioned media, tissues, serum, etc.) for endpoint determinations by the Projects. Aim 2: Perform SOL and EDL muscle functional testing of mice used in Projects 3-4. Aim 3: Perform microCT analysis to determine bone density and trabecular and cortical bone architectural properties used in Projects 2-4. Aim 4: Perform metabolomic and/or lipidomics analysis of mice from all exercise wheel running or other studies for Projects 1-4. Core C will coordinate microCT imaging, muscle function testing, exercise wheel running and metabolomic/lipidomic profiling technologies in support of the Projects. Core C will also have an important function with respect to providing training for students and postdoctoral fellows with the use of the provided technologies and the proper methods of data analysis for those methods. Core C will closely coordinate its activities with those of Core B in support of the 4 Projects. The efficiencies and cost savings achieved by this Core will greatly accelerate the discovery process of each Project and by providing a central platform, upon which these analyses are accomplished, allow for inter-project data comparisons. This Core is also essential for enhanced rigor and transparency of the overall PPG.

抽象的核心 C，即动物和分析核心，将支持该计划项目内所有子项目的各个方面格兰特对每个人的成功都至关重要。该计划项目的总体目标是了解机制肌肉和骨骼之间潜在的串扰可能导致与年龄相关的肌肉和骨骼衰退骨量和功能。拟议的项目还将研究肌肉骨骼串扰是否介导运动对肌肉骨骼系统的一些有益影响以及运动（自愿轮跑； VWR）可以在衰老过程中维持骨-肌肉串扰。该核心的具体目标是：目标 1：执行 VWR 实验、比目鱼肌 (SOL) 和趾长伸肌 (EDL) 肌肉收缩力对 VWR 和对照的影响项目 1-4 的小鼠并提供样品的集中处理和分发（条件培养基、组织、血清等）用于项目终点确定。目标 2：执行 SOL 和 EDL 肌肉功能测试项目 3-4 中使用的小鼠。目标 3：进行 microCT 分析以确定骨密度和小梁以及项目 2-4 中使用的皮质骨结构特性。目标 4：进行代谢组学和/或脂质组学对来自项目 1-4 的所有运动轮跑或其他研究的小鼠进行分析。 Core C 将协调 microCT 成像、肌肉功能测试、运动轮跑步和代谢组学/脂质组学分析技术项目的支持。核心C还将在为学生提供培训方面发挥重要作用和博士后研究员使用所提供的技术和正确的数据分析方法那些方法。核心 C 将与核心 B 密切协调其活动，以支持这 4 个项目。这该核心实现的效率和成本节约将大大加速每个项目的发现过程通过提供一个中央平台来完成这些分析，允许项目间数据比较。该核心对于增强整个 PPG 的严谨性和透明度也至关重要。