National Flow Cytometry and Sorting Research Resource

国家流式细胞术和分选研究资源

基本信息

批准号：
7802941
负责人：
BABETTA L MARRONE
金额：
$ 170.52万
依托单位：
LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-08-26 至 2012-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7802941
关键词：
Acoustics Address Advisory Committees Affinity Antigens Applications Grants Area Arts Authorship Automation Binding Biological Biological Assay Biology Biomedical Research Book Chapters Bromodeoxyuridine Cell Cycle Cells Cellular biology Chromosomes Clinical Clinical Research Collaborations Communities Complex Computer software Cytology Cytometry DNA Data Analyses Data Collection Detection Development Educational workshop Environment Expression Library Financial compensation Flow Cytometry Fluorescence Funding Genomics Goals Government Grant Hand Human Resources In Vitro Industry International Investigation Kinetics Laboratories Learning Left Legal patent Licensing Ligands Mails Measurement Measures Mentorship Methods Microspheres Molecular Multiparametric Analysis Mycobacterium tuberculosis New Mexico Newsletter Organelles Pamphlets Particle Size Pharmaceutical Preparations Phase Play Positioning Attribute Postdoctoral Fellow Preparation Process Process Measure Protein Engineering Protein p53 Proteins Proteomics Protocols documentation Publications Research Research Activity Research Personnel Resolution Resources Role Sampling Scanning Schools Scientist Screening procedure Services Small Business Innovation Research Grant Societies Sorting - Cell Movement Speed Students Surface Antigens System Techniques Technology Technology Transfer Temperature Testing Time Training Training Activity Training Programs United States National Institutes of Health Universities Update Validation Visit Work base data acquisition design digital experience flexibility graduate student high school high throughput analysis improved instrument instrumentation meetings member molecular assembly/self assembly new technology news novel novel strategies operation particle programs protein expression protein protein interaction research and development response single molecule stem structural genomics symposium technology development tool web page web site

项目摘要

DESCRIPTION (provided by the applicant): The NIH Roadmap Initiative has as one of its key underlying objectives the implementation of high through put, multiplexed instruments for application in a wide variety of basic and clinical research. The flow cytometry platform is ideally suited to this purpose, and several recent technologies have significantly increased throughput and simultaneous, multiparametric analysis. We propose to provide several critical technology advances by extending the capabilities and applications of flow-based analysis and sorting to produce an increasingly multiparameter, high-throughput analysis and screening tool. This will be accomplished through an integrated plan of three Research and Development Projects, extensive internal and external Collaborations, a wide variety of Service projects, expansion of our Training efforts, and increased Dissemination activities. The R&D Projects aim to extend flow cytometry into three areas of direct relevance to the Roadmap goals: 1) application of acoustic focusing and sensing technology to provide both novel analysis parameters and in-line sample preparation; 2) increasing both the throughput and the particle size range of flow sorting technologies; and 3) developing an integrated instrument to collect complete emission spectra separated according to fluorescence lifetime. Each of these projects has a defined set of collaborations that apply the new technologies to a wide range of biomedical research, including protein engineering, protein-protein interactions, drug and ligand screening, multiplexed cellular analysis, and automated preparation and analysis of complex clinical and environmental samples. In addition, we will continue and expand our Collaborative projects using instruments developed in the previous funding period. We will expand our Service efforts by providing access to a wide variety of commercial and unique developmental instruments. We will continue our traditionally outstanding Training program of hands-on courses and student/PostDoc mentorship, and develop new courses focused on application of our recent technology advances. Our strong Dissemination program will also be extended to include an improved website and the sponsorship of three focused meetings on various aspects of advanced flow cytometry. In conclusion, we propose to continue to drive the design, development, and application of flow cytometry technology as a multiplexed, high-throughput platform for advanced biomedical and clinical research.

描述（由申请人提供）：NIH路线图计划是其主要的基础目标之一，即通过PUT，多重仪器在各种基础和临床研究中应用多重仪器的实施。流式细胞仪平台非常适合此目的，最近的几种技术显着增加了吞吐量和同时进行的多参数分析。我们建议通过扩展基于流量的分析和排序的功能和应用来提供越来越多的多用量，高通量分析和筛选工具，以提供几种关键的技术进步。这将通过三个研究和开发项目，广泛的内部和外部合作，各种各样的服务项目，扩展我们的培训工作以及增加分发活动的综合计划来完成。研发项目旨在将流式细胞仪扩展到与路线图目标直接相关的三个领域：1）应用声学焦点和传感技术同时提供新的分析参数和在线样本准备； 2）增加流量排序技术的吞吐量和粒径范围； 3）开发一种集成仪器来收集根据荧光寿命分离的完整发射光谱。这些项目中的每一个都有一组定义的合作，将新技术应用于广泛的生物医学研究，包括蛋白质工程，蛋白质 - 蛋白质相互作用，药物和配体筛选，多路复用细胞分析以及复杂的临床和环境样品的自动化制备和分析。此外，我们将使用上一期资金期间开发的工具继续并扩展我们的协作项目。我们将通过提供各种商业和独特的开发工具来扩展我们的服务工作。我们将继续我们的动手课程和学生/博士后指导的传统上出色的培训计划，并开发针对我们最近技术进步的新课程。我们的强大传播计划还将扩展到包括改进的网站，并赞助三场有关高级流式细胞仪各个方面的集中会议。总之，我们建议继续推动流式细胞仪技术作为高级生物医学和临床研究的多重高通量平台的设计，开发和应用。