Identification and Functional Characterization of Zebrafish microRNAs

斑马鱼 microRNA 的鉴定和功能表征

基本信息

批准号：
7924413
负责人：
James G. Patton
金额：
$ 14.11万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-30 至 2011-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7924413
关键词：
3&apos Untranslated Regions Antisense Oligonucleotides Automobile Driving Bioinformatics Biotin Cell physiology Cells Cloning Code Data Defect Development Disease Embryo Embryonic Development Eukaryotic Cell Fishes Gastrula Gene Expression Gene Expression Regulation Gene Family Genes Genetic Screening Germ Layers Glean Goals Human In Situ Hybridization In Vitro Incubated Injection of therapeutic agent Label Mediating Messenger RNA MicroRNAs Normal Cell Northern Blotting Oligodendroglia Pattern Phenotype Protein Isoforms Proteins RNA Reporter Research Personnel Ribosomal RNA Role Sorting - Cell Movement System Testing Tissues Transfer RNA Transgenic Organisms Translational Repression Untranslated RNA Zebrafish base cell type gain of function gastrulation insight interest loss of function mutant nerve stem cell novel numb protein programs promoter research study streptavidin-agarose zebrafish development zygote

项目摘要

DESCRIPTION (provided by applicant): Multiple eukaryotic sequencing projects have led to the surprising finding that the number of protein coding genes is far less than that predicted based on total protein content. This underscores the fact that regulation of gene expression is crucial to enable a relatively limited number of genes to encode a wide array of protein isoforms in a tissue- and developmental-specific pattern. Within the last couple of years, it has become apparent that focusing on just protein coding genes ignores genes for noncoding RNAs which appear to make up more than 1% of the total number of genes. Several classes of RNA are well known (tRNA, mRNA, and rRNA) but perhaps the most interesting class of noncoding RNAs includes a family of genes referred to as microRNAs (miRNAs). miRNAs function to regulate gene expression in a sequence specific manner by either degrading target mRNAs or by mediating translational repression. In this proposal, we seek to globally identify all zebrafish miRNAs and determine their cell-, tissue-, and developmental-specific expression patterns using a novel microarray strategy. Eukaryotic cells encode approximately 250-300 miRNAs yet the targets of these RNAs are almost entirely unknown. Using the array data, we will identify miRNA targets and examine the phenotypic consequences of miRNA gain-of-function and loss-of-function. The fact that miRNAs have only recently been identified illustrates the difficulty of identifying all genes using forward genetic screens, especially small vertebrate genes such as those encoding miRNAs. It has been estimated that 10% or more of eukaryotic genes might be regulated by one or more miRNAs and the goal of this proposal is to use the zebrafish system to answer questions about how miRNAs regulate early development. Because miRNAs are highly conserved, it seems certain that information gleaned using zebrafish will be directly applicable to humans and uncover potential roles for human miRNAs in normal cell function, development, and disease.

描述（由申请人提供）：多个真核测序项目导致令人惊讶的发现，即蛋白质编码基因的数量远低于基于总蛋白质含量所预测的。这强调了以下事实：基因表达的调节对于使相对有限的基因能够在组织和发育特异性的模式中编码各种蛋白质同工型至关重要。在过去的几年中，很明显，仅关注仅蛋白质编码基因而忽略了非编码RNA的基因，而无编码的RNA似乎占基因总数的1％以上。几类RNA是众所周知的（tRNA，mRNA和rRNA），但也许最有趣的非编码RNA类别包括一个称为microRNA的基因家族（miRNA）。 miRNA功能通过降解靶mRNA或介导翻译抑制来以序列特定方式调节基因表达。在此提案中，我们寻求在全球范围内确定所有斑马鱼miRNA，并使用新型的微阵列策略来确定其细胞，组织和发育特异性表达模式。真核细胞编码约250-300个miRNA，但这些RNA的靶标几乎完全未知。使用阵列数据，我们将确定miRNA靶标并检查miRNA功能获得和功能丧失的表型后果。直到最近才发现miRNA的事实说明了使用正向遗传筛选（尤其是诸如编码miRNA的小脊椎动物基因）鉴定所有基因的困难。据估计，10％或更多的真核基因可能受一个或多个miRNA调节，该提案的目的是使用斑马鱼系统回答有关miRNA如何调节早期发展的问题。由于miRNA是高度保守的，因此似乎可以肯定的是，使用斑马鱼收集的信息将直接适用于人类，并发现人miRNA在正常细胞功能，发育和疾病中的潜在作用。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

miR-27 regulates chondrogenesis by suppressing focal adhesion kinase during pharyngeal arch development.

DOI：
10.1016/j.ydbio.2017.06.013
发表时间：
2017-09-01
期刊：
Developmental biology
影响因子：
2.7
作者：
Kara N;Wei C;Commanday AC;Patton JG
通讯作者：
Patton JG

Transcriptome-wide analysis of small RNA expression in early zebrafish development.

DOI：
10.1261/rna.029090.111
发表时间：
2012-05
期刊：
RNA
影响因子：
4.5
作者：
Chunyao Wei;Leonidas Salichos;Carli M. Wittgrove;A. Rokas;J. G. Patton
通讯作者：
Chunyao Wei;Leonidas Salichos;Carli M. Wittgrove;A. Rokas;J. G. Patton

The role of miR-124a in early development of the Xenopus eye.

miR-124a 在非洲爪蟾眼睛早期发育中的作用

DOI：
10.1016/j.mod.2009.08.002
发表时间：
2009-10
期刊：
MECHANISMS OF DEVELOPMENT
影响因子：
2.6
作者：
Qiu, Rong;Liu, Kaili;Liu, Ying;Mo, Weichuan;Flynt, Alex S.;Patton, James G.;Kar, Amar;Wu, Jane Y.;He, Rongqiao
通讯作者：
He, Rongqiao

miR-203 regulates progenitor cell proliferation during adult zebrafish retina regeneration.