ANALYTIC CYTOLOGY CORE FACILITY

分析细胞学核心设施

• 批准号: 8166187
• 负责人: JOANNE T ELLZEY
• 金额: $ 54.02万
• 依托单位: UNIVERSITY OF TEXAS EL PASO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8166187
• 关键词: Arsenic; Biological Sciences; Biology; Chemistry; Computer Retrieval of Information on Scientific Projects Database; Computer software; Contract Services; Core Facility; Cytology; Educational workshop; Electron Microscope; Electron Microscopy; Engineering; Equipment; Faculty; Fees; Funding; Goals; Grant; Health; Health Sciences; Heavy Metals; Image Analysis; Immunofluorescence Immunologic; Institution; Label; Mechanics; Microbiology; Microscope; Parasites; Printing; Productivity; Proteins; Protocols documentation; Research; Research Personnel; Resolution; Resources; Scanning; Scientist; Signal Transduction; Signaling Molecule; Site; Source; Techniques; Toxic effect; United States National Institutes of Health; Viral Proteins; Virulence Factors; design; digital; digital imaging; image processing; programs; transmission process; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The NIH-RCMI outside site reviewers rated the Analytical Cytology Core Facility Outstanding and recommended the hiring of an additional technician for electron microscopy and the establishment of users fees. The outside consultant, E. Ann Ellis recommended the hiring of a certified electron microscope technician, user fees and the purchase of a high resolution digital camera for the Zeiss EM-10 in order to expedite the provision of superb quality digital images. Immunolocalization of unique proteins by the use of immunofluorescence in the Zeiss Axioskop epifluorescence microscope or the Zeiss Pascal confocal scanning microscope and immunogold labeling utilizing the Zeiss EM-10 transmission electron microscope are essential techniques of high resolution for translational biology, the identification of parasites and their unique secretory products, viral proteins, bacterial and fungal virulence factors, mechanisms of heavy metal and arsenic toxicity, tumor identification, cell signaling molecules and many other techniques of the BBRC users who represent Biological Sciences, Health Sciences, Chemistry, Materials and Metallurgical Engineering and Mechanical and Industrial Engineering. This proposal provides for assistance in the designing of protocols and the use of sophisticated, well maintained and supervised equipment in the Analytical Cytology Core facility. Each microscope is equipped with software for image analysis, image processing and printing of digital images. Service contracts are maintained on each of the microscopes. Computers and software are regularly upgraded. Confocal workshops have been held regularly to familiarize users with new techniques and new protocols. Currently, there are thirty-two different projects utilizing this facility. In the last few years there has been a dramatic increase in the use of this facility. Greater use is anticipated with the hiring of new faculty who need cellular microbiology equipment. The facility provides essential equipment in support of the pathobiology emphasis of our doctoral program in Biological Sciences with the ultimate goal of research productivity in the study of border health by an interdisciplinary cooperation between scientists and engineers.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 NIH-RCMI外部现场审阅者对未销量的分析细胞学核心设施进行了评价，并建议聘用一名用于电子显微镜的技术人员和用户费用的建立。外部顾问E. Ann Ellis建议雇用认证的电子显微镜技术人员，用户费用和为Zeiss EM-10购买高分辨率数码相机，以加快提供优质数字图像的提供。通过在Zeiss axioskop的表面荧光显微镜中使用免疫荧光来对独特蛋白进行免疫定位，或利用Zeiss Zeiss em-10透射电子显微镜是高分辨率的基本技术，均具有翻译生物学的基本技术，并鉴定其鉴定，并独特的分泌产物，病毒蛋白，细菌和真菌毒力因子，重金属和砷毒性的机制，肿瘤识别，细胞信号分子以及代表生物学科学，化学，化学，材料和金属工程以及金属工程和金属工程以及金属科学的BBRC使用者的许多其他技术机械和工业工程。该建议为设计方案设计以及在分析细胞学核心设施中使用精致，维护良好和监督的设备的帮助提供了帮助。每个显微镜都配备了用于图像分析，图像处理和数字图像打印的软件。每个显微镜都维持服务合同。定期升级计算机和软件。经常举行共聚焦研讨会，以使用户熟悉新技术和新协议。目前，利用此设施有32个不同的项目。在过去的几年中，该设施的使用急剧增加。需要雇用需要细胞微生物设备的新教师的雇用，预计会有更多的使用。该设施提供了基本的设备，以支持我们在生物科学博士学位课程的病理学重点，其最终目标是研究生产力在边境健康研究中，科学家与工程师之间的跨学科合作。