Protein Microarray

蛋白质微阵列

基本信息

批准号：
7675504
负责人：
PHILIP Louis FELGNER
金额：
$ 14.22万
依托单位：
UNIVERSITY OF CALIFORNIA-IRVINE
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-05-01 至 2014-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7675504
关键词：
Animal Model Animals Antibodies Antigens Arboviruses Arizona Bartonella henselae Bioinformatics Biological Assay Cells Cloning Coccidioides Computer Analysis Computer Simulation Containment Core Protein Coupled Custom Dengue Development Devices Diagnostic Diagnostic tests Drug Formulations Early Diagnosis Emerging Communicable Diseases Enzyme-Linked Immunosorbent Assay Fluorescence Genome Genomics Genotype Goals Health Human Immunity Immunization Immunoassay Immunoglobulin G Immunological Diagnosis Infection Infectious Agent Infectious Diseases Research Institutes Laboratories Lateral Longevity Prevention Printing Protein Microchips Proteins Proteome Reading Research Personnel Sampling Scanning Serum Services Subunit Vaccines Surrogate Endpoint T-Lymphocyte Technology Testing Translating Universities Vaccines animal resource base biodefense cost data acquisition design fast protein liquid chromatography immunogenicity improved pre-clinical protein expression protein purification vaccine evaluation

项目摘要

The goal of the Protein Micro-Array Core is to provide investigators with a high throughput genome cloning platform from which protein microarrays can be made for discovery of vaccine and serodiagnostic antigens. The technology to make whole proteome microarrays is well established, and to date the Core has fabricated arrays containing 16,000 proteins from 20 different infectious agents. Antigens identified by array can be are purified and translated to other accessible serodiagnostic platforms, such as dot arrays in the bottom of 96 well plates (multiplex ELISA) or for the development of subunit vaccines. The also core offers probing and scanning, protein purification, fabrication of immunostrips /multiplex ELISAs (macroarrays in 96 well plates), and a full statistical analysis through the UCI Institute for Genomice and Bioinformatics. The core aims to expand on these capabilities by developing low cost DNA-baased genotyping and high throughput T cell antigen discovery. To support activities in the PSWRCE, the core will achieve the following aims Aim i: Provide microarray services and deliverables for new projects: a. Coccidioides posadasii (with John Galgiani, University of Arizona; Project 6.2) b. Bartonella henselae (with Jane Koehler, UCSF) Aim 2: Expand on existing platforms. a. Develop high throughput T cell antigen discovery (Coccidioides posadasii; Project 6.2). b. Develop low-cost DNA-based genotyping test (Dengue). Aim 3: Develop multiplex diagnostic tests to Arboviruses (with Carl Hanson, Immunoassay Development Services Core, and Paul Luciw Animal Resources and Laboratory Services Core). a. Immunostrips/Lateral flow devices b. Multiplex ELISA (Macro-arrays in 96-well plates)

蛋白质微阵列核心的目标是为研究人员提供高通量基因组克隆可以制作蛋白质微阵列以发现疫苗和血清诊断抗原的平台。制作全蛋白质组微阵列的技术已经很成熟，迄今为止，Core 已经制造出包含来自 20 种不同传染源的 16,000 个蛋白质的阵列。通过阵列鉴定的抗原可以是纯化并转化为其他可访问的血清诊断平台，例如 96 底部的点阵孔板（多重 ELISA）或用于亚单位疫苗的开发。该核心还提供探测和扫描、蛋白质纯化、免疫试纸条/多重 ELISA 的制作（96 孔宏阵列）板），以及通过 UCI 基因组和生物信息学研究所进行的完整统计分析。核心旨在通过开发低成本的基于 DNA 的基因分型和高通量 T 来扩展这些能力细胞抗原的发现。为了支持 PSWRCE 的活动，核心将实现以下目标目标一：为新项目提供微阵列服务和可交付成果：一个。 Coccidioides posadasii（与亚利桑那大学的 John Galgiani 合作；项目 6.2） b.汉赛巴尔通体（与 Jane Koehler，加州大学旧金山分校）目标 2：在现有平台上进行扩展。一个。开发高通量 T 细胞抗原发现（Coccidioides posadasii；项目 6.2）。 b.开发低成本的基于 DNA 的基因分型测试（登革热）。目标 3：开发针对虫媒病毒的多重诊断测试（与 Carl Hanson，免疫测定开发）服务核心、Paul Luciw 动物资源和实验室服务核心）。一个。免疫试纸/侧流装置 b.多重 ELISA（96 孔板中的宏阵列）