The Role of Pre-miRNA Loop in Target Regulation by microRNA Genes

Pre-miRNA 环在 microRNA 基因靶标调控中的作用

• 批准号: 7845907
• 负责人: CHANG-ZHENG CHEN
• 金额: $ 80万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7845907
• 关键词: Address; Affect; Animals; Award; Biological; Budgets; Caenorhabditis elegans; Code; Gene Deletion; Gene Expression; Gene Expression Regulation; Genes; Genetic Programming; Grant; Interview; Mediating; MicroRNAs; Nucleotides; Outcome; Play; Published Comment; RNA; Recommendation; Regulation; Regulatory Element; Review Committee; Role; Small RNA; United States National Institutes of Health; Vertebrates; abstracting; pre-miRNA; public health relevance; Address; Affect; Animals; Award; Biological; Budgets; Caenorhabditis elegans; Code; Gene Deletion; Gene Expression; Gene Expression Regulation; Genes; Genetic Programming; Grant; Interview; Mediating; MicroRNAs; Nucleotides; Outcome; Play; Published Comment; RNA; Recommendation; Regulation; Regulatory Element; Review Committee; Role; Small RNA; United States National Institutes of Health; Vertebrates; abstracting; pre-miRNA; public health relevance

DESCRIPTION Abstract MicroRNA (miRNA) genes encode an abundant class of ~22-nucleotide small RNAs that are thought to control gene expression at the post-transcriptional levels. Although the importance of miRNA-mediated gene regulation is now evident, as miRNA genes were shown to play diverse functional roles in animals, their mechanisms of action remain elusive. We have recently found that miRNA genes encoding identical mature miRNAs could have distinct biological activities that are determined by their cognate pre-miRNA loops, revealing unexpected regulatory complexity encoded in the pre-miRNA loops. These findings prompted us to reexamine some of the fundamental assumptions in the miRNA field. It was noted in the original discovery of the C. elegans lin-4 gene that mutations and deletions in lin-4 genes invariably affect including the primary Public Health Relevance Recent discoveries have revealed a fundamental layer of genetic programs controlled by an abundant class of RNA coding genes -- the miRNA genes. Here we will address the mechanisms by which the activity of miRNA genes may be controlled by the unexpected regulatory elements that we have discovered. THE FOLLOWING RESUME SECTIONS WERE PREPARED BY THE SCIENTIFIC REVIEW OFFICER TO SUMMARIZE THE OUTCOME OF DISCUSSIONS OF THE REVIEW COMMITTEE ON THE FOLLOWING ISSUES. VERTEBRATE ANIMAL (Resume): ACCEPTABLE COMMITTEE BUDGET RECOMMENDATIONS: The budget was recommended as requested. SCIENTIFIC REVIEW OFFICERS NOTES: Since the NIH Director's Pioneer Award applications are reviewed differently from other NIH grant mechanisms, criterion scores and percentiles are not assigned. The comments above represent a summary of the discussion by the interview panel. Please ignore the Administrative Budget Note on page one and the Notice below regarding resubmissions (formerly termed amended) applications as they do not apply to Pioneer Awards.

描述 抽象的 microRNA（miRNA）基因编码了一类〜22-核苷酸小的RNA，这些RNA被认为可以在转录后水平控制基因表达。尽管现在很明显miRNA介导的基因调节的重要性，但由于miRNA基因被证明在动物中起多种功能作用，但它们的作用机理仍然难以捉摸。我们最近发现，编码相同成熟的miRNA的miRNA基因可能具有不同的生物学活性，这些生物活性由它们的同源前miRNA环确定，揭示了在miRNA循环中编码的意外调节复杂性。这些发现促使我们重新检查了mirna领域中的一些基本假设。在原始发现秀丽隐杆线虫lin-4基因的原始发现中，Lin-4基因中的突变和缺失总是影响包括主要的 公共卫生相关性 最近的发现揭示了由大量的RNA编码基因（miRNA基因）控制的遗传程序的基本层。在这里，我们将解决miRNA基因活性可以由我们发现的意外调节元素控制的机制。 科学审查官员准备了以下简历部分，以总结以下问题审查委员会讨论的结果。 脊椎动物（简历）：可接受 委员会预算建议： 根据要求建议预算。 科学评论官员指出： 由于NIH董事的先驱奖励申请与其他NIH赠款机制的审查不同，因此未分配标准分数和百分位数。 上面的评论代表了访谈小组讨论的摘要。 请忽略第一页的行政预算说明以及以下关于重新提交（以前称为修订的）申请的通知，因为它们不适用于先锋奖。