Adminstrative Core

行政核心

• 批准号: 7662917
• 负责人: LINDA H SHAPIRO
• 金额: $ 11.46万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-26 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7662917
• 关键词: Arterial Fatty Streak; Atherosclerosis; Bone Marrow; Cell Separation; Cells; Clinic; Core Facility; Data; Development; Endothelial Cells; Ensure; Event; Financial Support; Future; Goals; Grant; Histology; Image Analysis; Immunohistochemistry; Individual; Inflammatory; Instruction; Lesion; Manuscripts; Modeling; Mus; Population; Preparation; Process; Protocols documentation; Quality Control; Reagent; Research; Research Personnel; Resources; Services; Signal Transduction; Techniques; Testing; Training; Vascular Diseases; experience; improved; macrophage; member; programs; research study; tissue processing; vascular inflammation

The Histology, Cell and Atheroma Core will provide five main services for investigators within the program project. 1) Tissue Processing and Immuno- and Histological Analysis, 2) Photo-documentation, image analysis and quantification and statistical testing of endpoint data for significance, 3) Mouse Lipidomic Analysis, 4) Primary endothelial cell isolation training and quality control for purity and 5) Isolation and culturing of bone marrow-derived macrophage training and reagents. The core has abundant experience and will coordinate with co-director, Dr. Jonathan Smith, Cleveland Clinic, on the detailed and quantitative analysis of atheroma lesions and murine lipidomics needed for these studies. Advanced histological processing and immunohistochemistry will be available to all projects. A key issue in the program goals has been the utilization of primary cell populations, primarily endothelial and bone marrow derived macrophage. Since different labs and individuals are currently isolating primary cells with variable levels of efficiency and application of quality control assessment of population purity, we have incorporated primary cell isolation and analysis into the core. The support from the core will allow investigators to develop projects and experiments without having technical difficulties with quantitative or cell isolation and analysis protocols is an integral component of the program project, provides expanded opportunities and goals for each project and will continue to be an indispensible resource for future efforts. RELEVANCE (See instructions): The Core Facility is necessary to support development of the individual projects. The application of specific techniques and models in a uniform manner will improve the interpretation of results, cross interpretation between projects and provide the most relevant information regarding the mechanisms of atherosclerosis and inflammatory events in vascular disease.

组织学，细胞和动脉瘤核心将为该计划中的调查人员提供五个主要服务 项目。 1）组织加工和免疫和组织学分析，2）图像，图像 端点数据的分析，定量和统计测试是否显着性，3）小鼠脂质组学 分析，4）主要的内皮细胞隔离训练和纯度的质量控制和5）隔离和 培养骨髓来源的巨噬细胞训练和试剂。核心具有丰富的经验， 将与克利夫兰诊所的乔纳森·史密斯（Jonathan Smith）博士协调 分析这些研究所需的动脉瘤病变和鼠脂肪组学。晚期组织学 所有项目都可以使用处理和免疫组织化学。计划目标中的一个关键问题 是原代细胞群体的利用，主要是内皮和骨髓衍生的巨噬细胞。 由于不同的实验室和个体目前正在隔离具有效率水平可变的原代细胞，并且 质量控制评估人口纯度的应用，我们已纳入了原发性细胞隔离和 分析核心。核心的支持将使调查人员能够开发项目和实验 不带有技术困难而没有定量或细胞隔离和分析方案是一个不可或缺的 计划项目的组成部分，为每个项目提供扩展的机会和目标，并将 继续成为未来努力的必不可少的资源。 相关性（请参阅说明）： 核心设施对于支持各个项目的开发是必要的。特定的应用 以均匀方式的技术和模型将改善结果的解释，交叉解释 在项目之间并提供有关动脉粥样硬化机制的最相关信息 和血管疾病中的炎症事件。