CORE B: High-throughput Genomic and RNA Analysis

核心 B：高通量基因组和 RNA 分析

基本信息

批准号：
7759244
负责人：
Lisa D White
金额：
$ 27.92万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7759244
关键词：
Achievement Advertisements Agar Gel Electrophoresis Alternative Splicing Apoptosis Archives Arts Base Sequence Bioconductor Bioinformatics Biological Biological Assay Biological Sciences Budgets Cells Chad Chromosomes Clinical Collaborations Color Communities Complementary DNA Complex Computer software Computers Consultations Core Facility Coupled Custom DNA DNA Fingerprinting DNA-Protein Interaction Data Data Analyses Databases Development Discipline Disease Educational workshop Environment Epigenetic Process Exons Experimental Designs Expressed Sequence Tags Faculty Fellowship Funding Gene Expression Gene Expression Profile Gene Expression Profiling Genes Genetic Predisposition to Disease Genome Genomics Genotype Glass Hand Hour Housing Human Human Cloning Human Genetics Human Genome Human Resources Hybridization Array Image Individual Instruction Intellectual functioning disability Label Laboratories Link Machine Learning Malignant Neoplasms Maps Mathematics Measurement Measures Medical Genetics Medical Students Medicine Mental Retardation and Developmental Disabilities Research Centers Methodology Methods Methylation MicroRNAs Microarray Analysis Modeling Molecular Molecular Profiling Mus Names National Cancer Institute National Eye Institute Neurologic Nucleic Acids Oligonucleotides Online Systems Pancreas Pathway Analysis Pathway interactions Pediatric Hospitals Physics Positioning Attribute Postdoctoral Fellow Price Protein Analysis Proteins Provider Publications Quality Control RNA Sequences RNA analysis Rattus Reaction Research Research Activity Research Institute Research Personnel Resources Retina Ribosomes Rice SNP genotyping Sampling Sequence Analysis Services Signal Transduction Silicon Spottings Students System Systems Analysis Technology Testing Texas Time Training Transcript Universities Work base chromatin remodeling college comparative genomic hybridization computer science cytokine data management design digital genome sequencing graduate student grasp instrument interest knowledge base mRNA Expression meetings member micro-total analysis system next generation novel promoter quality assurance repository research study single molecule tool transcriptomics web site

项目摘要

The High-throughput Genomic and RNA Analysis (HGRA) Core for the Baylor College of Medicine (BCM) Intellectual and Developmental Disabilities Research Center (IDDRC) utilizes the resources and knowledge base of the Microarray Core Facility (MCF) at BCM. This newly named core represents a combination of two previously funded IDDRC cores (Core B2: Genomic Array Core and Core C4: Expression Array Core). As a new activity, the core will also offer next generation sequencing technology to users. Originally the Genomic Array Core was set up to offer array comparative genomic hybridization (aCGH) to users of the BCM-IDDRC. Since that time the BCM Medical Genetics Laboratories (a CLIA certified lab environment) developed a targeted aCGH platform which is called Chromosomal Microarray Analysis (CMA). In 2006, Dr. Lisa D. White became Technical Director of the CLIA CMA lab and many of the BCM-IDDRC researchers began using that laboratory for their aCGH. The switch to the CLIA lab has been stunningly successful and of immense benefit to the IDD community as illustrated by the large numbers of publications listed below. A very large number of new genetic etiologies for IDD have resulted from these efforts. The HGRA will continue to offer aCGH as a research activity for members of the BCM-IDDRC as an adjunct to the clinical efforts. The HGRA core will combine cutting edge technologies to provide state-of-the-art quality microarray-based and next generation sequencing-based services and analyses for both transcriptional and genomic profiling. The MCF was established in 1999 with funds from BCM, the National Cancer Institute and the National Eye Institute to develop microarray resources for BCM researchers. With the restructuring of the HGRA core, our purpose expands to providing assistance to BCM-IDDRC researchers in utilizing microarray technology, next generation sequencing technology, good experimental design, and data management and data analysis resources. We will begin offering next generation sequencing technology (Illumina Genome Analyzer II) to BCM-IDDRC members in March 2009.

贝勒医学院的高通量基因组和 RNA 分析 (HGRA) 核心 (BCM) 智力和发育障碍研究中心 (IDDRC) 利用资源 BCM 的微阵列核心设施 (MCF) 和知识库。这个新命名的核心代表两个先前资助的 IDDRC 核心的组合（核心 B2：基因组阵列核心和核心 C4：表达式阵列核心）。作为一项新活动，该核心还将提供下一代为用户提供测序技术。基因组阵列核心最初的目的是提供阵列为 BCM-IDDRC 用户提供比较基因组杂交 (aCGH)。从那时起 BCM 医学遗传学实验室（CLIA 认证的实验室环境）开发了针对性的 aCGH 该平台称为染色体微阵列分析（CMA）。 2006 年，丽莎·D·怀特 (Lisa D. White) 博士成为 CLIA CMA 实验室的技术总监，许多 BCM-IDDRC 研究人员开始使用该实验室进行 aCGH。转向 CLIA 实验室取得了惊人的成功正如列出的大量出版物所示，它对 IDD 社区有巨大的好处以下。这些努力产生了大量新的 IDD 遗传病因。这 HGRA 将继续为 BCM-IDDDRC 成员提供 aCGH 作为一项研究活动，作为辅助临床工作。 HGRA 核心将结合尖端技术，提供最先进的品质基于微阵列和下一代测序的服务和分析转录和基因组分析。 MCF 成立于 1999 年，资金来自 BCM（美国国家癌症研究所和国家眼科研究所将为 BCM 开发微阵列资源研究人员。随着 HGRA 核心的重组，我们的目标扩展到提供协助 BCM-IDDDRC 研究人员利用下一代微阵列技术测序技术、良好的实验设计、数据管理和数据分析资源。我们将开始提供下一代测序技术（Illumina Genome 分析器 II) 于 2009 年 3 月向 BCM-IDDRC 成员提供。