Mouse Genetics

小鼠遗传学

基本信息

批准号：
7944902
负责人：
DAVID ANDREW LARGAESPADA
金额：
$ 13.39万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-08 至 2014-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7944902
关键词：
Advisory Committees Animals Arts Attention B-Cell Development Bacterial Artificial Chromosomes Biological Preservation Breeding Cancer Biology Cancer Center Cancer Center Support Grant Cells Cellular biology Colorectal Cancer Consultations Cryopreservation Cytogenetics Shared Resource DNA DNA Sequence Development Digestion Doctor of Philosophy Economic Inflation Electroporation Embryo Embryo Transfer Experimental Designs Fees Female Fertilization in Vitro Fibroblasts Gene Targeting Gene Transfer Techniques Genes Genetic Genetic Screening Harvest House mice Hybrids Immune response Immunity Immunology Implant In Vitro Inbred Strain Infant Injection of therapeutic agent Knock-out Knockout Mice Laboratories Life Maintenance Malignant Neoplasms Medicine Microinjections Minnesota Multiple Myeloma Mus Myeloid Leukemia Names Neoplasm Metastasis Oncogenes Plasmid Cloning Vector Pregnancy Preparation Production Protocols documentation Reagent Recombinants Research Research Personnel Resource Sharing Rodent Role Science Screening for cancer Screening procedure Services Silicon Dioxide Southern Blotting Specific Pathogen Frees Staging Surveillance Methods System Techniques Technology Testing Time Touch sensation Transgenes Transgenic Mice Transgenic Organisms Tumor Immunity Universities University of Minnesota Cancer Center Variant Work abstracting animal breeding anticancer research blastocyst comparative cost design embryo culture embryonic stem cell genetic manipulation germ free condition homologous recombination in vivo killings leukemia member mouse model offspring prevent professor programs research study restriction enzyme sperm cell stem success tool trait transmission process tumor tumor initiation

Advisory Committees Animals Arts Attention B-Cell Development Bacterial Artificial Chromosomes Biological Preservation Breeding Cancer Biology Cancer Center Cancer Center Support Grant Cells Cellular biology Colorectal Cancer Consultations Cryopreservation Cytogenetics Shared Resource DNA DNA Sequence Development Digestion Doctor of Philosophy Economic Inflation Electroporation Embryo Embryo Transfer Experimental Designs Fees Female Fertilization in Vitro Fibroblasts Gene Targeting Gene Transfer Techniques Genes Genetic Genetic Screening Harvest House mice Hybrids Immune response Immunity Immunology Implant In Vitro Inbred Strain Infant Injection of therapeutic agent Knock-out Knockout Mice Laboratories Life Maintenance Malignant Neoplasms Medicine Microinjections Minnesota Multiple Myeloma Mus Myeloid Leukemia Names Neoplasm Metastasis Oncogenes Plasmid Cloning Vector Pregnancy Preparation Production Protocols documentation Reagent Recombinants Research Research Personnel Resource Sharing Rodent Role Science Screening for cancer Screening procedure Services Silicon Dioxide Southern Blotting Specific Pathogen Frees Staging Surveillance Methods System Techniques Technology Testing Time Touch sensation Transgenes Transgenic Mice Transgenic Organisms Tumor Immunity Universities University of Minnesota Cancer Center Variant Work abstracting animal breeding anticancer research blastocyst comparative cost design embryo culture embryonic stem cell genetic manipulation germ free condition homologous recombination in vivo killings leukemia member mouse model offspring prevent professor programs research study restriction enzyme sperm cell stem success tool trait transmission process tumor tumor initiation

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项目摘要

9.6.1 ABSTRACT: MOUSE GENETICS LABORATORY Director: David Largaespada, Ph.D. The Mouse Genetics Laboratory (MGL) shared resource provides cancer center members with access to stateof- the-art technologies required to create and efficiently study genetically modified mice. Genetically modified mice have been vital tools for cancer studies for many years, allowing one to determine the role of specific genes in cancer-relevant traits such as the immune responses, tumor initiation, and metastasis to name a few. Therefore, access to these technologies is critical to the ongoing work of the University of Minnesota Cancer Center. Cancer Center members have used this shared resource to create mouse models of myeloid leukemia, multiple myeloma, colorectal cancer and infant leukemia. Transgenic mice produced by this shared resource have been used to create a system for performing forward genetic screens for cancer genes in mice. Members have studied aspects of T and B cell development and immunity using transgehic mice created by this shared resource. These studies have relevance for understanding tumor surveillance and methods to break tolerance for anti-tumor immunity. We anticipate that this shared resource will continue to serve investigators that require in vivo genetic confirmation of important hypotheses touching many aspects of cancer biology. The MGL has served the Cancer Center for 10 years, indeed since the first Cancer Center Support Grant (CCSG) application. The MGL provides many interrelated services that require manipulation of mouse embryos or embryonic stem (ES) cells. We perform pronuclear microinjection for the creation of transgenic mice, blastocyst injection for projects that involve genetic manipulation of ES cells, in vitro fertilization, sperm/embryo cyropreservation, and any service that requires manipulation of early mouse embryos. We recently added another service, which is ES cell gene targeting and expansion. The MGL provides free ES cells, drugresistant mouse embryo fibroblasts (MEFs), and plasmid vectors. Finally, we provide free consultation on mouse husbandry and strain maintenance, the design of ES cell gene targeting experiments, and the use of mouse models for cancer-related research in general. The MGL shared resource leader is Dr. David Largaespada, an Associate Professor in the Department of Genetics, Cell Biology and Development. He has been this shared resource's leader or co-leader since the beginning of the MGL and sets priorities for scientific use of the MGL in consultation with Cancer Center member users and the Rodent Advisory Committee (RAC) of the University of Minnesota. The MGL currently consists of three full time staff members in addition to Dr. Largaespada.

9.6.1摘要：鼠标遗传学实验室导演：David Largaespada博士小鼠遗传学实验室（MGL）共享资源为癌症中心成员提供了访问状态创建和有效研究基因修饰的小鼠所需的艺术技术。转基因多年来，小鼠一直是癌症研究的重要工具，从而确定特定的作用与癌症相关特征的基因，例如免疫反应，肿瘤起始和转移的基因。因此，获取这些技术对于明尼苏达大学持续的工作至关重要中心。癌症中心成员使用此共享资源来创建髓样白血病的鼠标模型，多发性骨髓瘤，结直肠癌和婴儿白血病。该共享资源产生的转基因小鼠已被用来创建一个用于在小鼠中为癌症基因进行正向遗传筛查的系统。成员已经研究了T和B细胞开发和免疫力的方面资源。这些研究与理解肿瘤监测和破坏公差的方法相关用于抗肿瘤免疫。我们预计该共享资源将继续为需要的调查人员服务重要假设的体内遗传确认，触及癌症生物学的许多方面。 MGL有自第一个癌症中心支持补助金（CCSG）以来，为癌症中心服务了10年应用。 MGL提供了许多相互关联的服务，需要操纵鼠标胚胎或胚胎（ES）细胞。我们对转基因小鼠的创建执行核微注射，胚泡注射针对涉及ES细胞基因操纵的项目，体外受精，精子/胚胎细胞保护和任何需要操纵早期小鼠胚胎的服务。我们最近添加另一种服务是ES细胞基因靶向和扩展。 MGL提供自由ES细胞，耐药性小鼠胚胎成纤维细胞（MEF）和质粒向量。最后，我们提供有关小鼠饲养和应变维护，ES细胞基因靶向实验的设计以及使用通常用于癌症相关研究的小鼠模型。 MGL共享资源负责人是David博士 Largaespada，遗传学，细胞生物学与发展系副教授。他有自从MGL开始以来，这种共享资源的领导者或共同领导者是科学的优先事项使用MGL与癌症中心成员用户和啮齿动物咨询委员会（RAC）协商明尼苏达大学。 MGL目前由三名全职工作人员组成。 Largaespada。