MACROMOLECULAR STRUCTURE

大分子结构

基本信息

批准号：
7944757
负责人：
ANDREW P HINCK
金额：
$ 3.19万
依托单位：
UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-08-03 至 2012-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7944757
关键词：
Arts Basic Science Binding Biological Cancer Center Complex Computer software Consultations Crystallization Data Data Collection Diffusion Doctor of Philosophy Educational workshop Gene Mutation Growth Image Isotope Labeling Label Laboratories Ligands Macromolecular Complexes Malignant Neoplasms Maps Measurement Methodology Methods Modeling Molecular Molecular Structure Mutation NMR Spectroscopy NOESY Nuclear Magnetic Resonance Nucleic Acids Online Systems Postdoctoral Fellow Preparation Prevention Process Proteins Research Personnel Residual state Resource Sharing Robot Roentgen Rays Sampling Services Solutions Structure Students Surface System Techniques Technology Therapeutic Therapeutic Agents Therapeutic Studies Training Training and Education X ray diffraction analysis X-Ray Crystallography X-Ray Diffraction cancer therapy computerized data processing instrumentation iterative design macromolecule member research study small molecule three dimensional structure vapor

项目摘要

The Macromolecular Structure Shared Resource (MSSR) is comprised of the X-ray crystallography (X-ray) and the Nuclear Magnetic Resonance (NMR) laboratories. X-ray crystallography and NMR are highly complementary methods for elucidating three-dimensional structures and for studying macromolecular interactions. Together, they provide Cancer Center members with comprehensive methodologies to understand how cancer-related biological macromolecules function in normal and diseased states at the molecular level. Cancer Center investigators will be advised and assisted in utilizing these sophisticated technologies to determine structures of cancer-related biological macromolecules and to investigate their interacfions with other macromolecules and with potential therapeutic agents by the Directors of the X-ray and NMR laboratories, P. John Hart, PhD and Andrew P. Hinck, PhD, respectively. The X-ray component of the MSSR includes an Art Robbins crystallization robot and two state-of-the-art Rigaku-MSSR X-ray data collecfion systems. The instrumentation provides full capabilities for conducting all modern X-ray diffraction experiments and is suitable for obtaining high quality three-dimensional structures of proteins, nucleic acids and their complexes. The NMR component of the MSSR includes state-of-the-art Bruker spectrometers equipped with high sensitivity cryoprobes operating at 500, 600, and 700 MHz. The instrumentafion provides full capabilities for conducting modern NMR experiments with N, C, and H labeled macromolecules and is suitable for obtaining three-dimensional solution structures and investigating interactions with other macromolecules and potential therapeutic agents. The MSSR is made accessible to the broader Cancer Center Membership by PhD-trained technical managers, Alex Taylor, PhD, and Udayar llangovan, PhD, for the X-ray and NMR laboratories, respectively, who provide guidance at each step in the process, from sample preparation to interpretation and presentation of results. The MSSR provides a comprehensive array of methodologies with which to visualize and functionally characterize cancer-related biological macromolecules.

大分子结构共享资源（MSSR）由X射线晶体学（X射线）组成以及核磁共振（NMR）实验室。 X射线晶体学和NMR高度阐明三维结构和研究大分子的互补方法互动。他们一起为癌症中心成员提供了全面的方法论了解与癌症相关的生物学大分子在正常状态和患病状态的起作用分子水平。癌症中心调查人员将得到建议并协助利用这些先进的确定与癌症相关生物学大分子的结构并研究它们的技术 X射线董事与其他大分子和潜在治疗剂的间隔 NMR实验室，P。JohnHart博士和Andrew P. Hinck，博士。 X射线组件 MSSR包括Art Robbins结晶机器人和两个最先进的Rigaku-MSSR X射线数据集合系统。该仪器提供了进行所有现代X射线衍射的全部功能实验，适合获得蛋白质，核酸的高质量三维结构及其复合物。 MSSR的NMR组件包括最先进的布鲁克光谱仪配备了高灵敏度的冷冻探针，在500、600和700 MHz下运行。仪器提供了使用N，C和H标记的H进行现代NMR实验的全部功能大分子，适合获得三维溶液结构并研究与其他大分子和潜在治疗剂的相互作用。 MSSR可访问由博士培训的技术经理，Alex Taylor，PhD和Udayar的更广泛的癌症中心会员资格 Llangovan博士分别为X射线和NMR实验室，他们在每个步骤中提供指导过程，从样本制备到结果的解释和呈现。 MSSR提供了综合的方法论可以可视化和功能表征与癌症相关的方法生物大分子。