GENOMICS SHARED RESOURCE

基因组学共享资源

基本信息

批准号：
7944742
负责人：
SUSAN L NAYLOR
金额：
$ 5.23万
依托单位：
UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-08-03 至 2012-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7944742
关键词：
Acids Aliquot Anticoagulants Arabidopsis Arts Aspirate substance Biological Assay Biological Markers Blood Blood specimen Bone Marrow Caenorhabditis elegans Cancer Center Canis familiaris Cattle Cell Line Cells Chickens Citrates Consult Culture Media Cultured Cells Custom Cytogenetics Shared Resource DNA DNA copy number Data Data Quality Databases Diagnosis Diagnostic Neoplasm Staging Discrimination Drosophila genus Experimental Designs Fluorochrome Freezing Funding Gene Deletion Gene Dosage Gene Expression Gene Expression Microarray Analysis Genes Genetic Genetic Polymorphism Genome Genomics Genomics Shared Resource Genotype Glucose Human Human Genome Human Herpesvirus 4 Industry Label Length Lymphocyte Macaca mulatta Maize Manuals Messenger RNA Methodology Methods Microarray Analysis Microarray Shared Resource Molecular Molecular Genetics Molecular Profiling Mononuclear Mononuclear Leukocytes Mus Nucleic Acids Oligonucleotides Peripheral Blood Mononuclear Cell Primer Extension Process Promega Protocols documentation RNA Rana Rattus Reader Relative (related person)Reproducibility Research Personnel Resource Sharing Resources Rice Running Sampling Services Single Nucleotide Polymorphism Solid Neoplasm Source Specimen Speed Technical Expertise Technology Testing Therapeutic Studies Time Tube Tumor Cell Line Tumor Tissue Venous Vial device Viral Whole Blood Yeasts Zebrafish anticancer research base cancer cell cancer therapy comparative genomic hybridization congenic cost cyanine dye 5 density experience genetic technology immortalized cell instrument interest lymphoblastoid cell line member neoplastic cell peripheral blood research study sex tissue culture tissue/cell culture trizol tumor

项目摘要

The Genomics Shared Resource provides state-of-the-art services to the members of the Cancer Center by providing access to two complementary platforms, the lllumina platform, used for primarily for highthroughput genotyping of single nucleotide polymorphisms, and the Agilent Technologies platform, used primarily for array comparative genomic hybridization and expression microarray analysis. In addition to these genomic services, the shared resource offers Cancer Center members assistance in automated nucleic acid isolation, real-time quantitative PCR, and sample banking. The banking of samples range from isolating DNA, processing blood specimens, establishing lymphoblastoid cell lines and long term storage of samples. The shared resource has been reorganized with a single overall coordinator and two co-directors, one per platform. All three have extensive experience in genomics and provide Cancer Center members with consulting services to assist in experimental design. This shared resource combines two previous funded cancer center shared resources, the Cytogenetic and Genetics Shared Resource and the Microarray Shared Resource.

基因组学共享资源通过提供对两个互补平台的访问权限，即Lllumina平台，主要用于高发射单核苷酸多态性的基因分型和使用的Agilent Technologies平台使用主要用于阵列比较基因组杂交和表达微阵列分析。此外这些基因组服务，共享资源为自动化提供了癌症中心成员的帮助核酸隔离，实时定量PCR和样品库。样品的押金范围从隔离DNA，加工血样，建立淋巴母细胞系和长期存储样品。共享资源已通过一个整体协调员和两个共同导演进行了重组，每个平台一个。这三者在基因组学方面都有丰富的经验，并为癌症中心成员提供提供咨询服务以协助实验设计。这个共享资源结合了两个以前的资助的癌症中心共享资源，细胞遗传学和遗传学共享资源和微阵列共享资源。