Oral Commensal Bacterial Modulation of the Periodontal Innate Host Response

口腔共生细菌对牙周先天宿主反应的调节

• 批准号: 7871479
• 负责人: Richard Peters Darveau
• 金额: $ 30.2万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-09 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7871479
• 关键词: Age; Animals; Bacteria; Binding; Cell Wall; Epithelial; Epithelial Cells; Fusobacterium nucleatum; Gene Expression Regulation; Genes; Genome; Germ-Free; Gingiva; Growth; Health; Hemin; Histologic; Homeostasis; Host Defense; Immune; Immune response; Immune system; Incubated; Integration Host Factors; Intestines; Life; Lipid A; Mediator of activation protein; Microarray Analysis; Molecular; Mus; Oral; Pattern; Periodontium; Play; Porphyromonas gingivalis; Proteins; Research Personnel; Role; Streptococcus; Streptococcus gordonii; System; TLR2 gene; TLR4 gene; Testing; Time; Tissues; base; commensal microbes; disorder prevention; microbial; microbial community; mutant; novel; oral bacteria; oral commensal; pathogen; periopathogen; programs; response

DESCRIPTION (provided by applicant): This application is based upon the premise that the innate host response to oral commensal colonization 5 beneficial to the host. In other words, the protective response elicited by the host to oral commensal bacterial colonization in fact contributes to normal periodontal tissue functions with respect to prevention of disease. It is known that clinically healthy periodontal tissue contains a highly orchestrated expression pattern of select innate host defense components that are believed to function in protecting this tissue and the host from pathogens. Studies in germ-free mice have revealed that commensal bacterial colonization of the intestine stimulates host responses that contribute to the ontogeny of the intestine with respect to immune and tissue function. Therefore, we wish to test the hypothesis that: "Commensal oral bacteria contribute to the ontogeny of the innate host response found in clinically healthy periodontal tissue". We will examine this hypothesis in three Specific Aims. Specific Aim 1 will compare the periodontal innate post defense status in germ-free and conventionally reared mice to identify those host components regulated by commensal oral bacteria. This Aim will determine the contribution of commensal bacteria to the ontogeny of the innate host response in the periodontium. Specific Aim 2 will determine if P. gingivalls, a well-known periopathogen can alter the "beneficial homeostasis" established between the host and its commensal flora. n Specific Aim 3 changes in gingival epithelial cell TLR2 and TLR4 expression levels that may alter commensal homeostasis with the host will be determined. Since both microbial composition and innate host response expression patterns are dynamic and likely to change over time these last two Aims are potentially relevant to alterations in oral bacterial I host interactions that may occur with increasing age.

描述（由申请人提供）：本申请基于这样的前提：宿主对口腔共生定植5的先天反应对宿主有益。换句话说，宿主对口腔共生细菌定植引起的保护性反应实际上有助于预防疾病的正常牙周组织功能。众所周知，临床健康的牙周组织含有精选的先天宿主防御成分的高度协调的表达模式，这些成分被认为具有保护该组织和宿主免受病原体侵害的作用。对无菌小鼠的研究表明，肠道内的共生细菌定植会刺激宿主反应，从而促进肠道在免疫和组织功能方面的个体发育。因此，我们希望检验以下假设：“共生口腔细菌有助于临床健康牙周组织中发现的先天宿主反应的个体发育”。我们将在三个具体目标中检验这一假设。具体目标 1 将比较无菌小鼠和传统饲养小鼠的牙周先天后防御状态，以确定受共生口腔细菌调节的宿主成分。该目标将确定共生细菌对牙周组织中先天宿主反应的个体发育的贡献。具体目标 2 将确定牙龈卟啉单胞菌（一种众所周知的牙周病原体）是否可以改变宿主与其共生菌群之间建立的“有益稳态”。 n 具体目标 3 将确定牙龈上皮细胞 TLR2 和 TLR4 表达水平的变化，这些变化可能会改变与宿主的共生稳态。由于微生物组成和先天宿主反应表达模式都是动态的，并且可能随着时间的推移而变化，这最后两个目标可能与随着年龄的增长而可能发生的口腔细菌 I 宿主相互作用的改变有关。

项目成果

Human enamel thickness and ENAM polymorphism.

• DOI: 10.1038/ijos.2016.1
• 发表时间: 2016-06-30
• 期刊: International journal of oral science
• 影响因子: 14.9
• 作者: Daubert DM;Kelley JL;Udod YG;Habor C;Kleist CG;Furman IK;Tikonov IN;Swanson WJ;Roberts FA
• 通讯作者: Roberts FA

The relationship of the oral microbiotia to periodontal health and disease.

• DOI: 10.1016/j.chom.2011.09.008
• 发表时间: 2011-10-20
• 期刊: Cell host & microbe
• 影响因子: 30.3
• 作者: Curtis MA;Zenobia C;Darveau RP
• 通讯作者: Darveau RP

Microbial shift and periodontitis.

• DOI: 10.1111/j.1600-0757.2010.00350.x
• 发表时间: 2011-02
• 期刊: Periodontology 2000
• 影响因子: 18.6
• 作者: Berezow AB;Darveau RP
• 通讯作者: Darveau RP

Impact of Oral Commensal Bacteria on Degradation of Periodontal Connective Tissue in Mice.

口腔共生细菌对小鼠牙周结缔组织降解的影响。

• DOI: 10.1902/jop.2015.150006
• 发表时间: 2015
• 期刊: Journal of periodontology
• 影响因子: 4.3
• 作者: Irie,Koichiro;Tomofuji,Takaaki;Ekuni,Daisuke;Morita,Manabu;Shimazaki,Yoshihiro;Darveau,RichardP
• 通讯作者: Darveau,RichardP