Transcriptome Characterization of Medicinal Plants Relevant to Human Health

与人类健康相关的药用植物的转录组表征

基本信息

批准号：
7943054
负责人：
RODNEY B CROTEAU
金额：
$ 129.06万
依托单位：
WASHINGTON STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-30 至 2012-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7943054
关键词：
Anabolism Animal Model Area Biochemical Biochemical Pathway Biochemistry Bioinformatics Biological Factors Biological Process Carbon Cell Culture Techniques Cells Chicago Classification Clinical Trials Code Communities Complex Computer software DNA Sequence Data Databases Deposition Development Engineering Enzymatic Biochemistry Enzyme Gene Enzymes Evaluation Foundations Gene Expression Profile Genes Genetic Genetic Transcription Genomics Goals Harvest Health Homologous Gene Housing Human Illinois Imagery Investigation Knowledge Lead Life Medicinal Plants Metabolic Metadata Modification Molecular Biology Molecular Genetics Molecular Profiling Museums Online Systems Orphan Outcome Pathway interactions Pharmaceutical Preparations Pharmacologic Substance Phytochemical Plant Sources Plants Process Production Public Health RNA Sequences Reaction Regulation Regulator Genes Reporting Research Research Infrastructure Research Personnel Resources Seasons Source Specificity Specimen Staging Structure Study models Technology Time Tissues Transcript Universities Validation Variant Work abstracting base cell type chemical synthesis comparative cost directed evolution drug development enzyme pathway gene discovery genetic variant genome wide association study improved novel public health relevance success synthetic biology tool transcription factor user-friendly voucher web-accessible

项目摘要

DESCRIPTION (provided by applicant): Plants are the source of many important medicinal compounds and the diversity of plant species and biochemistry suggests that many more are potentially available. The current understanding of the formation of plant-derived medicinal compounds at the enzyme, gene and regulatory levels is very incomplete-not a single complex plant medicinal pathway has yet to be completely elucidated at both the enzyme and the regulatory level. Historically, most studies of plant-derived medicinal compounds have been very narrowly focused, typically devoted to very specific steps in a particular biosynthetic pathway. These investigations were often pioneering-the diversity of plant biochemistry contains many novel reactions-but they were also very labor- intensive. More recently, genome-wide studies of model plant species have resulted in an explosive increase in our knowledge of, and capacity to understand, basic biological processes. Working from the genetics to the biochemistry now provides the most efficient way to build a long awaited and urgently needed foundation for more effectively probing and exploiting plant medicinal compound biosynthetic pathways. Having a comprehensive medicinal plant transcriptome database would propel medicinal plant species from an orphan- like status into the limelight of plant biochemistry and molecular genetics. The long-term goal of this research is for the scientific community to understand the complete formation, storage and regulation of plant-derived medicinal compounds at the enzyme and gene level. Improved understanding will enable the development of alternate sources of known pharmaceuticals and of novel drugs. The objective of this proposal is to provide the research community with urgently needed infrastructure and resources to enable comprehensive studies of the most compelling and medicinally significant plant biosynthetic pathways. Its Specific Aims are 1) To validate carefully selected medicinal plants based on taxonomic classification, medicinal compound accumulation and target transcript analysis, 2) To conduct transcriptome profiling of these medicinal plants and 3) To disseminate the accumulated data to the scientific community in the form of a user-friendly database. The resultant database and tools for comparative analysis will lead to gene discovery and enable technologies in the broader medicinal plant field. It is expected that the entire field of plant-derived pharmaceuticals will be advanced to a higher, more comprehensive level of analysis as a result of the proposed research. This is significant because the results will provide researchers in the field of plant-derived pharmaceuticals with a publicly available database and search tools for comparative analyses of pathway enzyme-coding genes and regulatory factors. These tools will enable gene discovery, metabolic engineering, synthetic biology and directed evolution for the improved production of drugs and for the development of novel drugs. Taken together, we envisage that this will ultimately result in tangible long-term and meaningful benefits for public health. PUBLIC HEALTH RELEVANCE: This research is directed toward understanding the various regulatory and biochemical factors that control medicinal compound formation, transport and storage in medicinal plants. Success in this area offers the promise of providing less expensive and/or novel medicinals that could positively impact the lives of hundreds of millions of people.

描述（由申请人提供）：植物是许多重要的药物化合物的来源，植物物种和生物化学的多样性表明，还有更多的可能可用。目前对酶，基因和调节水平在植物衍生的药物形成的理解是非常不完整的，没有单个复杂的植物药用途径在酶和调节水平上尚未完全阐明。从历史上看，大多数对植物衍生的药物化合物的研究都非常狭窄，通常专门针对特定生物合成途径的非常具体的步骤。这些调查通常是开创性的 - 植物生物化学的多样性包含许多新的反应，但它们也非常强大。最近，对模型植物物种的全基因组研究导致我们对基本生物学过程的了解和能力的爆炸性增加。现在，从遗传学到生物化学的工作现在提供了建立长期以来且迫切需要的基础的最有效方法，以更有效地探测和利用植物药物化合物复合生物合成途径。拥有全面的药用植物转录组数据库将使药物植物物种从孤儿状态促进植物生物化学和分子遗传学的焦点。这项研究的长期目标是让科学界了解酶和基因水平上植物衍生的药物化合物的完整形成，储存和调节。提高的理解将使已知药物和新药物的替代来源的发展发展。该提案的目的是为研究界提供迫切需要的基础设施和资源，以便对最引人注目，最重要的植物生物合成途径进行全面研究。它的具体目的是1）基于分类学分类，药用化合物积累和目标转录分析验证精心选择的药物植物，2）以用户友好的数据库的形式将这些药物的转录组分析和3）进行转录。最终的数据库和比较分析的工具将导致基因发现并在更广泛的药用植物领域中启用技术。预计，由于拟议的研究，整个植物衍生的药物的整个领域都将提高到更高，更全面的分析水平。这很重要，因为结果将为植物来源的药物领域的研究人员提供公开可用的数据库和搜索工具，以对途径酶编码基因和调节因素进行比较分析。这些工具将使基因发现，代谢工程，合成生物学和定向进化，以改善药物的产生和新型药物的开发。综上所述，我们设想，这最终将为公共卫生带来切实的长期和有意义的好处。公共卫生相关性：这项研究旨在理解控制药用植物中药用化合物形成，运输和储存的各种监管和生化因素。在这一领域的成功提供了提供较便宜和/或新颖的药用的希望，这些药物可能会对数亿人的生活产生积极影响。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Evaluation of reference genes for qRT-PCR studies in the colchicine producing Gloriosa superba L.

DOI：
10.1007/s11816-023-00840-x
发表时间：
2023-05-18
期刊：
Plant biotechnology reports
影响因子：
2.4
作者：
通讯作者：

Accurate mass-time tag library for LC/MS-based metabolite profiling of medicinal plants.

DOI：
10.1016/j.phytochem.2013.02.018
发表时间：
2013-07
期刊：
Phytochemistry
影响因子：
3.8
作者：
Cuthbertson DJ;Johnson SR;Piljac-Žegarac J;Kappel J;Schäfer S;Wüst M;Ketchum RE;Croteau RB;Marques JV;Davin LB;Lewis NG;Rolf M;Kutchan TM;Soejarto DD;Lange BM
通讯作者：
Lange BM

Cloning and characterization of a norbelladine 4'-O-methyltransferase involved in the biosynthesis of the Alzheimer's drug galanthamine in Narcissus sp. aff. pseudonarcissus.