Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution

近原子分辨率的螺旋蛋白和核蛋白聚合物的冷冻电镜

• 批准号: 10793162
• 负责人: EDWARD H. EGELMAN
• 金额: $ 2.3万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10793162
• 关键词: Acids; Actins; Bacteria; Biotechnology; Cryoelectron Microscopy; Filament; Food Poisoning; Funding; Health; Human; Ice; Meningitis; Muscle; Negative Staining; Nucleoproteins; Organism; Polymers; Preparation; Proteins; Resolution; Sampling; Structure; Virus; cost effective; hydrophilicity; repaired; tool

Abstract Supplementary funds are requested to purchase a glow discharge apparatus to be used nearly daily for the projects funded by this R35. Glow discharge of EM grids is needed for both negative stain and cryo-EM applications to clean the grids and make them more hydrophilic. This is a very important step in sample preparation, and is crucial to getting good ice for cryo-EM. The existing apparatus was purchased in my lab ~ 13 years ago, is failing to function properly, and cannot be repaired in any cost-effective way.

抽象的 需要补充资金购买几乎每天都会使用的辉光放电装置 由该 R35 资助的项目。负染色和冷冻电镜都需要电镜栅格的辉光放电 应用程序来清洁网格并使它们更加亲水。这是示例中非常重要的一步 准备工作，对于获得冷冻电镜所需的良好冰块至关重要。现有仪器是我实验室购买的~ 13 年前，该装置无法正常运行，并且无法以任何具有成本效益的方式进行修复。

项目成果

Cryo-EM structure of an extracellular Geobacter OmcE cytochrome filament reveals tetrahaem packing.

细胞外地杆菌 OmcE 细胞色素丝的冷冻电镜结构揭示了四血红素堆积。

• 发表时间: 2022-08
• 影响因子: 28.3
• 作者: Wang, Fengbin;Mustafa, Khawla;Suciu, Victor;Joshi, Komal;Chan, Chi H;Choi, Sol;Su, Zhangli;Si, Dong;Hochbaum, Allon I;Egelman, Edward H;Bond, Daniel R
• 通讯作者: Bond, Daniel R

The structures of two archaeal type IV pili illuminate evolutionary relationships.

两种古菌 IV 型菌毛的结构阐明了进化关系。

• 发表时间: 2020
• 影响因子: 16.6
• 作者: Wang, Fengbin;Baquero, Diana P;Su, Zhangli;Beltran, Leticia C;Prangishvili, David;Krupovic, Mart;Egelman, Edward H
• 通讯作者: Egelman, Edward H

Distinct axial and lateral interactions within homologous filaments dictate the signaling specificity and order of the AIM2-ASC inflammasome.

同源丝内不同的轴向和横向相互作用决定了 AIM2-ASC 炎症小体的信号传导特异性和顺序。

• 发表时间: 2021-05-12
• 影响因子: 16.6
• 作者: Matyszewski, Mariusz;Zheng, Weili;Lueck, Jacob;Mazanek, Zachary;Mohideen, Naveen;Lau, Albert Y;Egelman, Edward H;Sohn, Jungsan
• 通讯作者: Sohn, Jungsan

Spindle-shaped archaeal viruses evolved from rod-shaped ancestors to package a larger genome.

纺锤形古细菌病毒从杆状祖先进化而来，包装了更大的基因组。

• 发表时间: 2022-04-14
• 影响因子: 64.5
• 作者: Wang, Fengbin;Cvirkaite;Vos, Matthijn;Beltran, Leticia C;Kreutzberger, Mark A B;Winter, Jean;Su, Zhangli;Liu, Jun;Schouten, Stefan;Krupovic, Mart;Egelman, Edward H
• 通讯作者: Egelman, Edward H

Mutate or die: Atomic structures explain bacterial SOS induction.

突变或死亡：原子结构解释细菌 SOS 诱导。

• 发表时间: 2023-01-31
• 影响因子: 11.1
• 作者: Egelman; Edward H
• 通讯作者: Edward H