Request for administrative supplement to purchase a confocal microscope for project GM139971

申请行政补充，为项目 GM139971 购买共焦显微镜

基本信息

批准号：
10792740
负责人：
TIMOTHY L MEGRAW
金额：
$ 20万
依托单位：
FLORIDA STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-03-01 至 2025-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10792740
关键词：
Adipocytes Administrative Supplement Animals Architecture Basement membrane Binding Biochemistry Biological Assay Cell physiology Cells Cellular biology Centrosome Collagen Complex Confocal Microscopy Cytoskeleton Data Development Disease Drosophila genus Dynein ATPase Endosomes Equipment Fat Body Funding Future Generations Goals Grant Homeostasis Interphase Cell Knowledge Location Maps Measures Membrane Proteins Microscope Microtubule-Organizing Center Microtubules Modeling Molecular Molecular Structure Motor Nature Nuclear Organ Organism Orthologous Gene Physiology Polymerase Positioning Attribute Printing Process Protein Isoforms Publications Publishing Radial Regulation Research Role Structure Surface Testing Tubulin Vesicle Work aged cell type experimental study frontier human disease infancy instrument member ninein novel programs recruit synergism trafficking

项目摘要

Our current project (R01 GM139971) is focused on elucidating the mechanisms by which non-centrosomal microtubule-organizing centers (ncMTOCs) function. This is important because many, if not most, non-dividing cells in the organism rely on ncMTOCs rather than the centrosome, the better understood animal MTOC. Considered a new frontier in cytoskeleton research, ncMTOCs are diverse in their structures, subcellular locations, and mechanisms of microtubule assembly. My lab has discovered three distinct ncMTOCs in the past few years, and in this project we focus on discerning the mechanisms by which a perinuclear MTOC in Drosophila adipocytes is assembled and by which it controls microtubule assembly. To achieve the aims of this grant and future projects, confocal microscopy is needed to execute and evaluate experiments and is the most critical core piece of equipment for my research program. The general and ongoing need for intensive confocal microscopy use is reflected in all of our publications in the past 13 years, where we have had ready access to a Nikon A1 confocal microscope housed adjacent to our lab. This instrument is used daily by members of my lab and is now aged, slow and in disrepair. A supplement to fund a new confocal microscope is necessary to assure progress with this project and allow us to retire the current instrument.

我们当前的项目（R01 GM139971）专注于阐明机制非中心体微管组织中心（ncMTOC）通过其发挥作用。这很重要，因为生物体内有许多（如果不是大多数）非分裂细胞依赖 ncMTOC 而不是中心体，这是更好理解的动物 MTOC。 ncMTOC 被认为是细胞骨架研究的新领域其结构、亚细胞位置和微管机制各异集会。我的实验室在过去几年中发现了三种不同的 ncMTOC，在这个项目中，我们专注于辨别机制果蝇脂肪细胞中的核周 MTOC 是通过组装形成的控制微管组装。为了实现本次赠款和未来的目标项目中，需要共焦显微镜来执行和评估实验是我的研究项目中最关键的核心设备。这对密集共焦显微镜使用的普遍和持续的需求反映在所有方面过去 13 年我们发表的出版物中，我们可以随时访问 Nikon A1 共焦显微镜位于我们实验室附近。该仪器是我的实验室成员每天都在使用它，但现在已经老化、缓慢且年久失修。一个为了确保进展，有必要补充资助新的共焦显微镜与这个项目并允许我们退役当前的仪器。

项目成果

期刊论文数量（2）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.

微管和微管组织中心协调寨卡病毒感染和病毒质组织。

DOI：
发表时间：
2021-11-27
期刊：
影响因子：
6
作者：
Buchwalter, Rebecca A;Ogden, Sarah C;York, Sara B;Sun, Li;Zheng, Chunfeng;Hammack, Christy;Cheng, Yichen;Chen, Jieyan V;Cone, Allaura S;Meckes Jr, David G;Tang, Hengli;Megraw, Timothy L
通讯作者：
Megraw, Timothy L

Evidence for intraflagellar transport in butterfly spermatocyte cilia.

蝴蝶精母细胞纤毛鞭毛内运输的证据。