Characterization of non-canonical regulatory pathways in the Caulobacter NtrYX signaling system

柄杆菌 NtrYX 信号系统中非典型调控途径的表征

• 批准号: 10794646
• 负责人: Benjamin J Stein
• 金额: $ 5.09万
• 依托单位: UNIVERSITY OF TENNESSEE CHATTANOOGA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-20 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10794646
• 关键词: Administrative Supplement; Agriculture; Bacteria; Biochemical; Caulobacter; Caulobacter crescentus; Complex; Culture Media; Development; Educational process of instructing; Environment; Funding; Future; Genetic; Genetic Transcription; Goals; Health; Homeostasis; Human; Infection; Laboratories; Methods; Microbe; Modeling; Organism; Preparation; Proliferating; Proteins; Regulation; Regulatory Pathway; Research; Signal Pathway; Signal Transduction; Sterility; Sterilization; System; Work; antimicrobial; environmental change; experience; meetings; microbial; parent grant; parent project; success

Project Summary/Abstract All bacteria must sense and respond to internal and external signals to thrive in diverse environments. This sensing is often achieved using protein-based two-component signaling systems. Our funded parent project is developing the Caulobacter crescentus NtrY-NtrX (NtrYX) two-component signaling system as a model for understanding complex bacterial signaling pathways. Our aims seek to understand this atypical two-component signaling system at multiple levels, from regulation of signal flow to changes in transcription. Although our aims employ diverse biochemical and genetic methods, at their core they all require bacterial culture. Thus, a functional and reliable autoclave for the preparation of growth media, sterile glassware, and sterile consumables is key for the success of our project goals. This proposal seeks an administrative supplement to replace our broken autoclave with a functional, high quality, and high-capacity autoclave appropriate for our laboratory space. This new autoclave will be capable of meeting all sterilization demands for our parent grant and will also help support additional microbial-related research and teaching in our department.

项目概要/摘要 所有细菌都必须感知内部和外部信号并对其做出反应，才能在不同的环境中繁衍生息。 这种传感通常是使用基于蛋白质的双组分信号系统来实现的。我们资助的母项目 正在开发 Caulobacter crescentus NtrY-NtrX (NtrYX) 双组分信号系统作为模型 了解复杂的细菌信号传导途径。我们的目标是了解这种非典型的两部分 多个层面的信号系统，从信号流的调节到转录的变化。虽然我们的目标 采用不同的生化和遗传学方法，其核心都需要细菌培养。因此，一个 功能可靠的高压灭菌器，用于制备生长培养基、无菌玻璃器皿和无菌耗材 是我们项目目标成功的关键。该提案寻求行政补充以取代我们的 破碎的高压灭菌器，具有适合我们实验室的功能齐全、高质量、大容量的高压灭菌器 空间。这个新的高压灭菌器将能够满足我们家长补助金的所有灭菌要求，并且还将 帮助支持我们系的其他微生物相关研究和教学。