Shared Resource in Cellular Imaging

细胞成像共享资源

• 批准号: 10721473
• 负责人: Anju Vasudevan
• 金额: $ 28.33万
• 依托单位: HUNTINGTON MEDICAL RESEARCH INSTITUTES
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10721473
• 关键词: Acceleration; Biomedical Research; Cell physiology; Cellular biology; Core Facility; Education; Equilibrium; Floor; Fluorescence; Funding; Future; Goals; Grant; Heart Diseases; Huntington Disease; Image; Immunofluorescence Immunologic; Medical Research; Mental disorders; Microscope; Microscopic; Outsourcing; Postdoctoral Fellow; Psychiatric therapeutic procedure; Research; Research Institute; Research Personnel; Research Support; Resource Sharing; Speed; Subcellular Anatomy; Technology; United States National Institutes of Health; Vision; cellular imaging; cost; fluorescence microscope; forging; imaging system; instrument; meetings; multidisciplinary; nervous system disorder; programs; undergraduate student

Abstract The vision of the Cellular Imaging Core of the Huntington Medical Research Institutes (HMRI) is to provide investigators access to cutting-edge technology, and expert technical support for microscopic observation and analysis of cellular anatomy and physiology that is essential for diverse aspects of our multidisciplinary biomedical research. A major gap in our current imaging systems is acquiring a balance between image quality, throughput, and accessibility. Due to these limitations, researchers at HMRI need to outsource their brightfield and immunofluorescence needs to costly and busy core facilities. Here we propose to overcome these workflow and high-cost limitations by proposing an all-in-one fluorescence microscope, BZ-X800 that has multiple features and will strengthen the cell biology projects in various labs at HMRI. The instrument will be housed in the Cellular Imaging Core facility designated to the second floor of the newly built research building and will accelerate research supported by 5 NIH R01 grants, on robust and quantitative fluorescence and brightfield imaging. The Core will be shared by multiple investigators and will strengthen the undergraduate, STEM and postdoctoral educational programs at HMRI. It will be critical for meeting the goals of ongoing NIH-funded projects, as well as planned future projects. This all-in-one microscope will facilitate our research at unprecedented speeds, and forge new avenues for understanding and treatment of psychiatric, neurological, and heart disease.

抽象的 亨廷顿医学研究所 (HMRI) 细胞成像核心的愿景是 为调查人员提供尖端技术和专家技术支持 细胞解剖学和生理学的显微观察和分析对于 我们多学科生物医学研究的各个方面。我们当前成像的一个主要差距 系统正在图像质量、吞吐量和可访问性之间取得平衡。由于 这些限制，HMRI 的研究人员需要外包他们的明场和 免疫荧光需要昂贵且繁忙的核心设施。在这里我们建议克服 通过提出一体式荧光显微镜来克服这些工作流程和高成本限制， BZ-X800 具有多种功能，将加强各个实验室的细胞生物学项目 磁共振成像。该仪器将安装在指定用于 新建研究大楼二楼，将加速由 5 个项目支持的研究 NIH R01 资助稳健、定量的荧光和明场成像。核心将 由多个研究人员共享，并将加强本科生、STEM 和 HMRI 的博士后教育项目。这对于实现持续的目标至关重要 NIH 资助的项目以及计划的未来项目。这种一体式显微镜将有助于 我们以前所未有的速度进行研究，并为理解和探索开辟新途径 治疗精神科、神经科和心脏病。