Synergistic effects of conjugated linoleic acid and calcium on bone mass

共轭亚油酸和钙对骨量的协同作用

基本信息

批准号：
7811660
负责人：
Yeonhwa Park
金额：
$ 15.21万
依托单位：
UNIVERSITY OF MASSACHUSETTS AMHERST
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-30 至 2011-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7811660
关键词：
Adipocytes Adverse effects Affect Animals Applications Grants Body fat Bone Marrow Bone Marrow Stem Cell Calcium Clinical Trials Conjugated Linoleic Acids Development Diet Dietary Calcium Dietary Component Disease Elderly Environment Equilibrium Equipment Food Food Additives Foundations Future Goals Health Health Benefit Human Human Resources Incidence Industry Intervention Isomerism Knowledge Lead Massachusetts Mediator of activation protein Mesenchymal Stem Cells Mission Modeling Molecular Mus Nature Obesity Osteoblasts Osteogenesis Osteoporosis Osteoporosis prevention Ovariectomy Postdoctoral Fellow Prevention Prevention strategy Quality of life Reporting Research Research Infrastructure Research Personnel Research Project Grants Role Staging Students Supplementation System Techniques Testing Texas Time United States Universities Wood material Work animal tissue base bioactive food component bone bone health bone loss bone mass bone metabolism calcium intake design dietary supplements effective intervention experience food science high risk human disease improved innovation insight knock-down lipid biosynthesis mortality nutrition prevent public health relevance research study stem cell differentiation success tissue culture tool

项目摘要

DESCRIPTION (provided by applicant): One of the most important interventions for osteoporosis is prevention of bone loss. The main emphasis of prevention strategies has been on dietary calcium intake, however calcium by itself has limited efficacy. Thus, any component that can improve calcium's effect on bone mass can improve bone health significantly. Conjugated linoleic acid (CLA) may be a good candidate for controlling osteoporosis based on the observation that CLA reduces body fat while increasing lean mass along with total ash. However, the reported effects of CLA on bone mass have not been consistent. We hypothesized that the inconsistency of CLA with regard to bone mass is due to differences in dietary calcium content. My long term goal in the original application is to contribute to the development of strategies designed to reduce bone loss through dietary and supplementary based interventions. The objective in the original R21 grant application is to evaluate the extent to which dietary CLA influence bone metabolism with dietary calcium in an experimental osteoporosis model. Based on the original application, we would like to expand our scope of study by adding a new hypothesis that inhibition of adipogenesis by CLA is a significant but not critical contributing factor for CLA's beneficial effects on osteogenesis. The rationale for this revised application is that investigating the role of CLA specifically on balancing adipogenesis and osteogenesis due to the pluripotential nature of mesenchymal stem cells will help understand the critical aspects of CLA in regard to application for eventual use in human health. This revised study will determine if controlling of adipogenesis by CLA is critical for CLA's impact on bone health. Thus, the research in the requested revision is relevant to that part of NIH's mission that pertains to developing knowledge that will potentially help to reduce human diseases. The new hypotheses will be tested by pursuing a new Specific Aim: (3) Determine the role of CLA in the balance between osteogenesis and adipogenesis in bone marrow mesenchymal stem cells. By using mesenchymal stem cells knocked-down for target mediators of osteogenesis and adipogenesis, we will focus on identifying the role of CLA on adipogenesis that can directly influence osteogenesis or vice versa. The scope of the overall project is to investigate the underlying role of CLA on bone mass improvement, which may lead to the application ofCLA as a dietary supplement or food additive for prevention of bone loss. The anticipated contribution of the requested revision is that the revised application will contribute to comprehensively integrate and expand new insight for understanding the interrelationship between osteoblasts and adipocytes from mesenchymal stem cells as well as the potential use of CLA to prevent osteoporosis. The Public Health relevance of this project is that identifying any dietary components that can improve bone health will have a significant impact on reduction and/or prevention of osteoporosis, which can lead to an improved quality of life, particularly in the elderly. PUBLIC HEALTH RELEVANCE: Osteoporosis is a disease characterized by low bone mass, with the elderly particularly susceptible, and it affects the quality of life as well as increasing mortality, due to complications. A unique prevention strategy by controlling balance of bone formation and fat cell formation from bone marrow stem cell by conjugated linoleic acid can potentially be a very useful tool for preventing the development of osteoporosis, leading to improved quality of life particularly in the elderly.

描述（由申请人提供）：骨质疏松症最重要的干预措施之一是预防骨质流失。预防策略的主要重点是饮食钙摄入量，但是钙本身具有有限的功效。因此，任何可以改善钙对骨骼质量影响的成分都可以显着改善骨骼健康。结合的亚油酸（CLA）可能是控制骨质疏松症的良好候选者，因为观察到CLA会减少体内脂肪，同时与总灰分一起增加瘦质量。但是，CLA对骨骼质量的效果尚不一致。我们假设CLA在骨骼质量方面的不一致是由于饮食中钙含量的差异所致。我在原始应用中的长期目标是为制定旨在通过饮食和补充干预措施减少骨质流失的策略做出贡献。原始R21赠款应用的目的是评估饮食中饮食中饮食中饮食代谢的程度，在实验性骨质疏松模型中。基于原始应用，我们希望通过添加一个新的假设来扩大研究范围，即CLA抑制脂肪形成是对CLA对成骨的有益影响的重要但不是关键的因素。这项修订的应用的理由是，由于间充质干细胞的多元电位性质，研究CLA在平衡脂肪形成和成骨方面的作用将有助于理解CLA在最终在人类健康中的最终使用方面的关键方面。这项修订的研究将确定CLA对脂肪形成的控制是否对于CLA对骨骼健康的影响至关重要。因此，所请求的修订中的研究与NIH使命的那部分有关，该任务与发展有助于减少人类疾病的知识有关。新假设将通过追求新的特定目的来检验：（3）确定CLA在骨骨髓间充质干细胞中成骨和脂肪形成之间平衡的作用。通过使用对成骨和脂肪形成的目标介体敲除击倒的间充质干细胞，我们将集中精力确定CLA对脂肪形成的作用，该作用可以直接影响骨生成，反之亦然。整个项目的范围是研究CLA在骨骼质量改善中的潜在作用，这可能导致CLA作为饮食补充剂或食物添加剂的应用，以预防骨质流失。所请求的修订的预期贡献是，修订后的应用程序将有助于全面整合和扩展新的见解，以理解间质干细胞的成骨细胞和脂肪细胞之间的相互关系，以及CLA的潜在用途，以防止骨质造成骨质造成骨质的骨质病。该项目的公共卫生相关性是，确定任何可以改善骨骼健康的饮食成分都将对减少和/或预防骨质疏松症产生重大影响，这可以改善生活质量，尤其是在老年人中。公共卫生相关性：骨质疏松症是一种以低骨骼为特征的疾病，老年人特别容易受到影响，并且由于并发症而影响生活质量以及死亡率的提高。通过控制骨髓形成和通过共轭亚油酸从骨髓干细胞形成骨骼形成和脂肪细胞形成的独特预防策略可能是防止骨质疏松症发展的非常有用的工具，从而改善了生活质量，尤其是在老年人中。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Conjugated linoleic acid and calcium co-supplementation improves bone health in ovariectomised mice.

DOI：
10.1016/j.foodchem.2012.12.067
发表时间：
2013-09-01
期刊：
FOOD CHEMISTRY
影响因子：
8.8
作者：
Park, Yooheon;Kim, Jonggun;Scrimgeour, Angus G.;Condlin, Michelle L.;Kim, Daeyoung;Park, Yeonhwa
通讯作者：
Park, Yeonhwa

trans-10,cis-12 CLA promotes osteoblastogenesis via SMAD mediated mechanism in bone marrow mesenchymal stem cells.