Targeted and traceable delivery of siRNA with nanoparticle-amphipol

使用纳米颗粒-amphipol 进行 siRNA 的靶向和可追踪递送

基本信息

批准号：
7696064
负责人：
Xiaohu Gao
金额：
$ 32.37万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-08-12 至 2011-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7696064
关键词：
Acids Address Amines Animals Area Biology Biomedical Engineering Cardiovascular Diseases Cell Culture Techniques Cell membrane Cells Charge Chemicals Clinical Research Complex Culture Media Cytoplasm Detection Development Dyes Electron Microscope Electrons Encapsulated Endosomes Engineering Ensure Environment Fluorescence Fostering Gene Silencing Generations Genetic Goals Gold Image Kinetics Label Life Lipid Bilayers Lipofectamine Liposomes Malignant Neoplasms Medicine Messenger RNA Molecular Nanostructures Nanotechnology Particle Size Peptides Photobleaching Plague Polyethyleneimine Preparation Property Proteins Quantum Dots RNA RNA Interference RNA Stability RNA Transport Resolution Serum Signal Transduction Silicon Dioxide Site Small Interfering RNA Specificity Structure Surface Technology Therapeutic Time TimeLine Toxic effect Viral Work base biodegradable polymer biological research cancer therapy cell fixing college cytotoxicity design fluorophore gene function human disease in vivo innovation interest medical schools nanocarrier nanoparticle nanoscale nervous system disorder particle prevent self assembly tool transmission process

项目摘要

Silencing RNA (siRNA) is of considerable current interest in biological research because it can elicit potent, target specific knockdown of any mRNA, creating a useful and proven genetic surrogate tool. However, a key problem for effective application of this technology is specific delivery to targeted cells. The goal of this project is to develop a new generation of nanocarriers for efficient delivery of siRNA based on our recent discovery on nanoparticle-amphipol complexes. In the two year project period, we will systematically examine the chemical and structural properties of nanoparticles and their effects on siRNA delivery in cells. We will focus on fluorescent quantum dots as a discovery tool. If successful, the proposed work will aid in development of siRNA carriers for specific treatment of cancer.

沉默 RNA (siRNA) 目前在生物学研究中引起了相当大的兴趣，因为它可以引发对任何 mRNA 的有效、靶向特异性敲除，从而创建一种有用且经过验证的遗传替代工具。然而，有效应用该技术的一个关键问题是将其特异性递送至目标细胞。该项目的目标是基于我们最近对纳米颗粒-amphipol 复合物的发现，开发新一代纳米载体，用于有效递送 siRNA。在为期两年的项目期间，我们将系统地研究纳米颗粒的化学和结构特性及其对细胞内siRNA递送的影响。我们将重点关注荧光量子点作为发现工具。如果成功，拟议的工作将有助于开发用于癌症特异性治疗的 siRNA 载体。