Using Nanomaterials to Inhibit Mast Cell/Basophil-Associated Disease

使用纳米材料抑制肥大细胞/嗜碱性粒细胞相关疾病

• 批准号: 7892398
• 负责人: Chris L KEPLEY
• 金额: $ 29.5万
• 依托单位: LUNA INNOVATIONS, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-21 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7892398
• 关键词: Acute; Affect; Allergic; Allergic Disease; Allergic inflammation; American; Anaphylaxis; Antigens; Arthritis; Arts; Asthma; Autoantibodies; Autoimmune Diseases; Basophils; Biological; Biological Sciences; Carbon; Cells; Development; Diagnosis; Disease; Effector Cell; Evaluation; Experimental Autoimmune Encephalomyelitis; Experimental Models; Foundations; Fullerenes; Goals; Growth; Human; Hypersensitivity; IgE; In Vitro; Inflammation; Inflammatory; Interdisciplinary Study; Joints; K/BxN model; Kinetics; Link; Mediating; Methodology; Modeling; Molecular; Monitor; Multiple Sclerosis; Mus; Nanotechnology; Natural Immunity; Pathology; Pathway interactions; Peptides; Principal Investigator; Process; Property; Protein Microchips; Public Health; Research; Science; Shapes; Signal Pathway; Soccer; Stem Cell Factor; Stimulus; Structure; Testing; allergic response; cell type; chemical property; human disease; improved; in vitro Model; in vivo; mast cell; multidisciplinary; nanomaterials; novel; oligodendrocyte-myelin glycoprotein; peripheral blood; programs; research study; response

DESCRIPTION (provided by applicant): Nanotechnology, the use of nanomaterials at the molecular level, is a multidisciplinary scientific field undergoing exponential growth and has broad applications among all divisions of science. One form of nanomaterials, fullerenes, is soccer-ball-shaped carbon cages that can be functionalized and derivatized with a wide array of molecules. Given their unique structure and properties, fullerenes are being investigated as a new and/or improved way to diagnose, monitor, and treat certain conditions. However, no studies have examined the effects these compounds have on the allergic response. Mast cells (MC) and peripheral blood basophils (PBB) are important effector cells that have traditionally been associated with initiating and propagating the allergic response. Recent studies suggest that these cells may also be important regulators of inflammation and innate immunity. We have discovered an unexpected and new mechanism through which the allergic process is inhibited using fullerenes. We hypothesize that fullerene nanomaterials may be a heretofore unrecognized, and potentially useful, way to inhibit diseases that are critically affected by MC and PBB responses. Further, we hypothesize these molecules may be manipulated in such a way that allows them to specifically target MC and PBB. To test these hypotheses we will use a combination of primary human MC and PBB in addition to murine models of human disease including allergy, arthritis, and multiple sclerosis. Our goal is to determine if fullerenes can inhibit the induction and progression of those diseases that have previously been shown to have a large MC/PBB influence. This completely novel research program may lay the foundation for new strategies not only for allergy treatment but for those diseases that are mediated by MC and PBB responses such as arthritis and autoimmune disease. Project Narrative: Allergic diseases, arthritis, multiple sclerosis, and other autoimmune diseases are huge public health problems that affect millions of Americans. These diseases are induced and propagated largely by mast cells and basophils. We have discovered that certain nanomaterials block their noxious effects and thus may be a new way to control those diseases that are largely mediated by these cells types.

描述（由申请人提供）：纳米技术，分子层的纳米材料的使用，是一个经历指数增长的多学科科学领域，并且在所有科学的所有部门中都有广泛的应用。纳米材料的一种形式是富勒烯，是足球形的碳笼，可以用各种分子进行功能化和衍生化。鉴于它们的独特结构和特性，正在研究富勒烯作为一种新的和/或改进的诊断，监测和治疗某些疾病的方法。但是，尚无研究检查这些化合物对过敏反应的影响。肥大细胞（MC）和外周血嗜碱性粒细胞（PBB）是重要的效应细胞，传统上与​​启动和传播过敏反应有关。最近的研究表明，这些细胞也可能是炎症和先天免疫的重要调节剂。我们发现了一种出乎意料的新机制，通过该机制使用富勒烯抑制过敏过程。我们假设富勒烯纳米材料可能是迄今未识别且可能有用的方法来抑制受到MC和PBB反应严重影响的疾病。此外，我们假设这些分子可以通过使它们专门针对MC和PBB的方式进行操纵。为了检验这些假设，除了鼠类疾病的鼠模型，包括过敏，关节炎和多发性硬化症，我们还将使用原代人MC和PBB的组合。我们的目标是确定富勒烯是否可以抑制以前证明具有较大MC/PBB影响的疾病的诱导和进展。 这个完全新颖的研究计划不仅可以为过敏治疗，而且是由MC和PBB反应（例如关节炎和自身免疫性疾病）介导的那些疾病的新策略的基础。 项目叙述：过敏性疾病，关节炎，多发性硬化症和其他自身免疫性疾病是影响数百万美国人的巨大公共卫生问题。这些疾病主要由肥大细胞和嗜碱性疾病诱导和传播。我们发现某些纳米材料阻止了其有害作用，因此可能是控制这些细胞类型主要介导的疾病的一种新方法。