Purchase of a Cryo-Probe to Upgrade a 500 MHz NMR System for BioNMR Applications

购买冷冻探针来升级 500 MHz NMR 系统以用于 BioNMR 应用

• 批准号: 7591326
• 负责人: Om Prakash
• 金额: $ 31.61万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7591326
• 关键词: Alzheimer' s Disease; Amyloid beta-Protein; Arts; Ataxia; Binding; Biomedical Research; Biomolecular Nuclear Magnetic Resonance; Carbon; Chitin; Collaborations; Cystic Fibrosis; Data; Dementia; Detection; Disease; Disease Vectors; Distant; Funding; Glucans; Health; Hour; Human; Immunity; Inflammatory; Insect Proteins; Insecta; Institution; Ischemia; Kansas; Lysine; N.I.H. Research Support; Noise; Nucleocapsid Proteins; Peptides; Performance; Proteins; Protons; Research Personnel; Research Project Grants; SARS coronavirus; Sampling; Signal Transduction; Sodium Chloride; Solubility; Solutions; Structure; System; Tertiary Protein Structure; Time; United States National Institutes of Health; Universities; cryogenics; human disease; improved; instrument; instrumentation; programs; public health relevance; research study

DESCRIPTION (provided by applicant): The Kansas State University (KSU) Biomolecular NMR Facility, which has served more than 23 separate NIH-funded research projects, request funding to purchase a 5 mm triple resonance cryogenic probe with enhanced carbon sensitivity and salt tolerant proton coil for an existing Varian 500 NMR System(R) . This acquisition of a cryogenic probe will afford a 2-3 fold enhancement in sensitivity compared with conventional probes and will be of great use to eight major users and many other NIH supported investigators at this campus studying mass-limited biomolecules having less stability and/or low solubility and biomacromolecules that show concentration-dependent aggregation. Currently our users are constrained with the present instrumentation due to the limited sensitivity of the conventional probe. Many of our local researchers supported by NIH funding are forced to go to distant facilities for 2D and 3D NMR studies on their diluted samples, as our 500 MHz NMR spectrometer equipped with a conventional probe is limited in its ability to perform these experiments in a timely manner and in some cases not at all. The cryogenic probe is essential for our 500 MHz console to perform long hours 2D and 3D NMR experiments for studying solution structure and dynamics of large biomolecules and other compounds available in limited amount. The leading performance benefits of the upgraded 500 MHz NMR spectrometer with the requested cryogenic probe will be: a) improved signal-to-noise ratio in the NMR spectra, b) reduced experimental time for multi-dimensional NMR experiments, c) lowered detection limit, and d) enhanced capability to acquire NMR data on diluted samples and on biomacromolecules with low solubility and or less stability. In brief this upgrade will facilitate KSU Biomolecular NMR facility in performing multi-dimensional NMR experiments on proteins/peptides and other biomacromolecules available in low concentration with best possible sensitivity on the existing instrument. The funding of this project will provide a shared instrumentation necessary to complete on going NIH supported research programs at KSU and to elevate inter-institutional biomedical research collaborations between KSU NMR facility and nearby institutions. PUBLIC HEALTH RELEVANCE: Most of the research projects in this application aim at understanding the structure and function of a variety of protein domains relevant to human health. These include chitin binding domain and 2-1, 3 glucan binding domain of insect proteins, lysine-rich domain of the SARS- CoV nucleocapsid protein, channel forming peptides, amyloid beta peptides and biophysical mechanism of protein-protein or protein-peptide interactions, particularly in immunity and inflammatory diseases and in insects as disease vectors. The acquisition of a state-of-the-art cryogenic probe for our existing 500 MHz NMR console will significantly enhance or create new feasibility of such studies of broad importance in a number of human diseases, such as Alzheimer's dementia, ataxia, ischemia, and cystic fibrosis. .

描述（由申请人提供）：堪萨斯州立大学（KSU）生物分子NMR设施，该设施已提供23多个独立的NIH资助的研究项目，请求资金购买5 mm三重谐振的低温探针，具有增强的碳敏感性和耐盐性蛋白质现有Varian 500 NMR系统（R）的线圈。与常规探针相比，对低温探针的这一获得的敏感性将增强2-3倍的敏感性，并且在该校园里对八个主要用户和许多其他NIH支持的研究人员非常有用，研究了质量限制的生物分子，其稳定性和/或低溶解度和显示浓度依赖性聚集的生物ac骨分子。目前，由于常规探针的敏感性有限，我们的用户受到本仪器的限制。我们的许多由NIH资金支持的本地研究人员被迫去遥远的设施进行稀释样品进行2D和3D NMR研究，因为我们的500 MHz NMR光谱仪配备了常规探测器的能力有限方式，在某些情况下根本没有。低温探针对于我们的500 MHz控制台进行长时间的2D和3D NMR实验至关重要，以研究溶液结构和大型生物分子的动力学和其他化合物的动力学。使用要求的低温探针升级的500 MHz NMR光谱仪的领先性能优势将是：a）NMR光谱中提高信噪比的提高，b）降低了多维NMR实验的实验时间，c）降低检测限量，d）增强了在稀释的样品和具有低溶解性和稳定性较低的生物百形分子上获取NMR数据的能力。简而言之，此升级将促进KSU生物分子NMR功能，以对蛋白质/肽和其他生物乳清分子进行多维NMR实验，以低浓度可用，并对现有仪器进行最佳敏感性。该项目的资金将为KSU的NIH支持研究计划提供必要的共同工具，并提升KSU NMR设施与附近机构之间的机构间生物医学研究合作。公共卫生相关性：本应用程序中的大多数研究项目旨在了解与人类健康相关的各种蛋白质领域的结构和功能。这些包括几丁质结合结构域和2-1，昆虫蛋白的3个葡萄糖结合结构域，Sars-CoV核​​素蛋白的赖氨酸富域，通道形成肽，淀粉样蛋白β肽以及蛋白质蛋白质或蛋白质肽相互作用，蛋白质蛋白质相互作用，淀粉样蛋白β肽的机制，特别是在免疫和炎症性疾病和作为疾病媒介的昆虫中。对我们现有的500 MHz NMR控制台的最先进的低温探测器将显着增强或创造这种广泛重要性的新可行性，这些研究在许多人类疾病中，例如阿尔茨海默氏症，共济失调，缺血，缺血和诸如阿尔茨海默氏症囊性纤维化。 。