Purchase of a Cryo-Probe to Upgrade a 500 MHz NMR System for BioNMR Applications

购买冷冻探针来升级 500 MHz NMR 系统以用于 BioNMR 应用

• 批准号: 7591326
• 负责人: Om Prakash
• 金额: $ 31.61万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7591326
• 关键词: Alzheimer' s Disease; Amyloid beta-Protein; Arts; Ataxia; Binding; Biomedical Research; Biomolecular Nuclear Magnetic Resonance; Carbon; Chitin; Collaborations; Cystic Fibrosis; Data; Dementia; Detection; Disease; Disease Vectors; Distant; Funding; Glucans; Health; Hour; Human; Immunity; Inflammatory; Insect Proteins; Insecta; Institution; Ischemia; Kansas; Lysine; N.I.H. Research Support; Noise; Nucleocapsid Proteins; Peptides; Performance; Proteins; Protons; Research Personnel; Research Project Grants; SARS coronavirus; Sampling; Signal Transduction; Sodium Chloride; Solubility; Solutions; Structure; System; Tertiary Protein Structure; Time; United States National Institutes of Health; Universities; cryogenics; human disease; improved; instrument; instrumentation; programs; public health relevance; research study

DESCRIPTION (provided by applicant): The Kansas State University (KSU) Biomolecular NMR Facility, which has served more than 23 separate NIH-funded research projects, request funding to purchase a 5 mm triple resonance cryogenic probe with enhanced carbon sensitivity and salt tolerant proton coil for an existing Varian 500 NMR System(R) . This acquisition of a cryogenic probe will afford a 2-3 fold enhancement in sensitivity compared with conventional probes and will be of great use to eight major users and many other NIH supported investigators at this campus studying mass-limited biomolecules having less stability and/or low solubility and biomacromolecules that show concentration-dependent aggregation. Currently our users are constrained with the present instrumentation due to the limited sensitivity of the conventional probe. Many of our local researchers supported by NIH funding are forced to go to distant facilities for 2D and 3D NMR studies on their diluted samples, as our 500 MHz NMR spectrometer equipped with a conventional probe is limited in its ability to perform these experiments in a timely manner and in some cases not at all. The cryogenic probe is essential for our 500 MHz console to perform long hours 2D and 3D NMR experiments for studying solution structure and dynamics of large biomolecules and other compounds available in limited amount. The leading performance benefits of the upgraded 500 MHz NMR spectrometer with the requested cryogenic probe will be: a) improved signal-to-noise ratio in the NMR spectra, b) reduced experimental time for multi-dimensional NMR experiments, c) lowered detection limit, and d) enhanced capability to acquire NMR data on diluted samples and on biomacromolecules with low solubility and or less stability. In brief this upgrade will facilitate KSU Biomolecular NMR facility in performing multi-dimensional NMR experiments on proteins/peptides and other biomacromolecules available in low concentration with best possible sensitivity on the existing instrument. The funding of this project will provide a shared instrumentation necessary to complete on going NIH supported research programs at KSU and to elevate inter-institutional biomedical research collaborations between KSU NMR facility and nearby institutions. PUBLIC HEALTH RELEVANCE: Most of the research projects in this application aim at understanding the structure and function of a variety of protein domains relevant to human health. These include chitin binding domain and 2-1, 3 glucan binding domain of insect proteins, lysine-rich domain of the SARS- CoV nucleocapsid protein, channel forming peptides, amyloid beta peptides and biophysical mechanism of protein-protein or protein-peptide interactions, particularly in immunity and inflammatory diseases and in insects as disease vectors. The acquisition of a state-of-the-art cryogenic probe for our existing 500 MHz NMR console will significantly enhance or create new feasibility of such studies of broad importance in a number of human diseases, such as Alzheimer's dementia, ataxia, ischemia, and cystic fibrosis. .

描述（由申请人提供）：堪萨斯州立大学（KSU）生物分子NMR设施，该设施已提供23多个独立的NIH资助研究项目，要求资金购买具有增强的碳敏感性的5 mm三重共振低温探针，并具有增强的碳耐药性和盐耐受剂的Proton Coil，用于现有的Varian 500 NMR系统（R）。与传统探针相比，对低温探针的这种获取将具有2-3倍的敏感性增强，并且对八个主要用户和许多其他NIH支持的研究人员非常有用，该校园研究质量受限的生物分子具有较小的稳定性和/或低的阳光性和生物乳酸菌的浓度浓度依赖性浓度依赖性依赖性。目前，由于常规探针的敏感性有限，我们的用户受到本仪器的限制。我们的许多由NIH资助支持的本地研究人员被迫去遥远的设施进行稀释样品研究，因为我们的500 MHz NMR光谱仪配备了常规探测器的500 MHz NMR光谱仪的能力有限，其能力及时执行这些实验的能力，在某些情况下完全不是。低温探针对于我们的500 MHz控制台进行长时间的2D和3D NMR实验至关重要，以研究溶液结构和大型生物分子的动力学和其他化合物的动力学。 The leading performance benefits of the upgraded 500 MHz NMR spectrometer with the requested cryogenic probe will be: a) improved signal-to-noise ratio in the NMR spectra, b) reduced experimental time for multi-dimensional NMR experiments, c) lowered detection limit, and d) enhanced capability to acquire NMR data on diluted samples and on biomacromolecules with low solubility and or less 稳定。简而言之，此升级将促进KSU生物分子NMR功能，以对蛋白质/肽和其他生物乳清分子进行多维NMR实验，以低浓度可用，并对现有仪器进行最佳敏感性。该项目的资金将为KSU的NIH支持研究计划提供必要的共同工具，并提升KSU NMR设施与附近机构之间的机构间生物医学研究合作。公共卫生相关性：本应用程序中的大多数研究项目旨在了解与人类健康相关的各种蛋白质领域的结构和功能。其中包括昆虫蛋白质的几丁质结合结构域和2-1个，3个葡萄糖结合结构域，SARS-COV核素蛋白富含赖氨酸的结构域，通道形成的肽，淀粉样β肽以及蛋白质蛋白质或蛋白质肽相互作用的生物型机制，尤其是在免疫和炎症中疾病和昆虫疾病中的抗病患者和昆虫症。我们现有的500 MHz NMR控制台的最先进的低温探测器将显着增强或创造这种广泛重要性的新可行性，这些研究在许多人类疾病中，例如阿尔茨海默氏症的痴呆症，双在血性，缺血，缺血，缺血和静脉纤维化。 。